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| | {{Template:Moscow_RF}} | | {{Template:Moscow_RF}} |
| − | <html lang="en" dir="ltr" class="client-nojs"> | + | <html> |
| − | <head>
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| − | </head>
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| − | <body class="mediawiki ltr sitedir-ltr ns-0 ns-subject page-Team_Moscow_RF skin-igem action-view">
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| − | <script type='text/javascript' src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
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| − | <script type='text/javascript' src ='/wiki/skins/Igem/resources/2017_skin.js'></script>
| + | <div class="column full_size" > |
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| − | <div id='globalWrapper'>
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| − | <div id='top_menu_under' class='noprint'></div>
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| − | <div id='top_menu_14' class='noprint'>Loading menubar.....</div> <!-- Will be replaced with the jQuery.load -->
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| − | <script>jQuery('#top_menu_14').load('https://2017.igem.org/cgi/top_menu_14/menubar_reply.cgi',
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| + | |
| − | </script>
| + | |
| − | | + | |
| − | <!-- Content div contains HQ_page for HQ styles, Logo and title div, and USER CONTENT -->
| + | |
| − | <div id="content" class="mw-body" role="main">
| + | |
| − | <a id="top"></a>
| + | |
| − | | + | |
| − | <div id="top_title">
| + | |
| − | <div class="logo_2017">
| + | |
| − | <a href="https://2017.igem.org">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/8/8b/HQ_page_logo.jpg" width="100px">
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| − | </a>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <h1 id="firstHeading" class="firstHeading">
| + | |
| − | <span dir="auto">Team:Moscow RF</span>
| + | |
| − | </h1>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div id="HQ_page">
| + | |
| − | <div id="bodyContent">
| + | |
| − | <div id="mw-content-text" lang="en" dir="ltr" class="mw-content-ltr"><p>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <script>
| + | |
| − | | + | |
| − | // This is the jquery part of your template.
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| − | // Try not modify any of this code too much since it makes your menu work.
| + | |
| − | | + | |
| − | $(document).ready(function() {
| + | |
| − | | + | |
| − | $("#HQ_page").attr('id','');
| + | |
| − | | + | |
| − | // call the functions that control the menu
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| − | menu_functionality();
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| − | hide_show_menu();
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | //this function controls the expand and collapse buttons of the menu and changes the +/- symbols
| + | |
| − | function menu_functionality() {
| + | |
| − | | + | |
| − | //when clicking on a "menu_button", it will change the "+/-" accordingly and it will show/hide the corresponding submenu
| + | |
| − | $(".menu_button").click(function(){
| + | |
| − | | + | |
| − | // add or remove the class "open" , this class holds the "-"
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| − | $(this).children().toggleClass("open");
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| − | // show or hide the submenu
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| − | $(this).next('.submenu_wrapper').fadeToggle(400);
| + | |
| − | });
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| − | | + | |
| − | // when the screen size is smaller than 800px, the display_menu_control button appears and will show/hide the whole menu
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| − | $("#display_menu_control").click(function(){
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| − | $('#menu_content').fadeToggle(400);
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| − | });
| + | |
| − | | + | |
| − | // call the current page highlight function
| + | |
| − | highlight_current_page();
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | // call the highlight current page function to show it on the menu with a different background color
| + | |
| − | function highlight_current_page() {
| + | |
| − | | + | |
| − | // select a page from the menu based on the id assigned to it and the current page name and add the class "current page" to make it change background color
| + | |
| − | $("#"+ wgPageName.substring(wgPageName.lastIndexOf("/")+1, wgPageName.length ) + "_page").addClass("current_page");
| + | |
| − | | + | |
| − | // now that the current_page class has been added to a menu item, make the submenu fade in
| + | |
| − | $(".current_page").parents(".submenu_wrapper").fadeIn(400);
| + | |
| − | // change the +/- symbol of the corresponding menu button
| + | |
| − | $(".current_page").parents(".submenu_wrapper").prev().children().toggleClass("open");
| + | |
| − |
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | // allow button on the black menu bar to show/hide the side menu
| + | |
| − | function hide_show_menu() {
| + | |
| − |
| + | |
| − | // in case you preview mode is selected, the menu is hidden for better visibility
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| − | if (window.location.href.indexOf("submit") >= 0) {
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| − | $(".igem_2017_menu_wrapper").hide();
| + | |
| − | }
| + | |
| − | | + | |
| − | // if the black menu bar has been loaded
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| − | if (document.getElementById('bars_item')) {
| + | |
| − | | + | |
| − | // when the "bars_item" has been clicked
| + | |
| − | $("#bars_item").click(function() {
| + | |
| − | $("#sideMenu").hide();
| + | |
| − | | + | |
| − | // show/hide the menu wrapper
| + | |
| − | $(".igem_2017_menu_wrapper").fadeToggle("100");
| + | |
| − | });
| + | |
| − | }
| + | |
| − | | + | |
| − | // because the black menu bars loads at a different time than the rest of the page, this function is set on a time out so it can run again in case it has not been loaded yet
| + | |
| − | else {
| + | |
| − | setTimeout(hide_show_menu, 15);
| + | |
| − | }
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | });
| + | |
| − | | + | |
| − | | + | |
| − | </script>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <style>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /***************************************************** DEFAULT WIKI SETTINGS ****************************************************/
| + | |
| − |
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| − | /* Clear the default wiki settings */
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| − |
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| − | #home_logo, #sideMenu { display:none; }
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| − | #sideMenu, #top_title, .patrollink {display:none;}
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| − | #content { width:100%; padding:0px; margin-top:-7px; margin-left:0px;}
| + | |
| − | body {background-color:white; }
| + | |
| − | #bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
| + | |
| − | | + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | /**************************************************************** MENU ***************************************************************/
| + | |
| − | /* Wrapper for the menu */
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| − | .igem_2017_menu_wrapper {
| + | |
| − | width: 15%;
| + | |
| − | height:100vh;
| + | |
| − | position:fixed;
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| − | right:0%;
| + | |
| − | padding:0px;
| + | |
| − | float:right;
| + | |
| − | border-left: 1px solid #d3d3d3;
| + | |
| − | background-color:#ffffff;
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| − | text-align:left;
| + | |
| − | font-family:Tahoma, Geneva, sans-serif;
| + | |
| − | overflow-y: auto;
| + | |
| − | overflow-x: hidden;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* this hides the scrollbar to keep view consistency */
| + | |
| − | .igem_2017_menu_wrapper:-webkit-scrollbar {
| + | |
| − | display: none;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | /* styling for links in the menu, removes the line under text */
| + | |
| − | .igem_2017_menu_wrapper a {
| + | |
| − | text-decoration: none;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* styling for the images in the menu */
| + | |
| − | .igem_2017_menu_wrapper img {
| + | |
| − | width: 100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* styling for the menu buttons */
| + | |
| − | .igem_2017_menu_wrapper .menu_button {
| + | |
| − | width: 100%;
| + | |
| − | padding: 10px 0px 10px 15px;
| + | |
| − | float:left;
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| − | border-bottom: 1px solid #d3d3d3;
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| − | font-size: 13px;
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| − | font-weight: bold;
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| − | color: #000000;
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| − | cursor: pointer;
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| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2017_menu_wrapper .menu_bottom_padding {
| + | |
| − | width: 100%;
| + | |
| − | height: 30px;
| + | |
| − | float:left;
| + | |
| − | }
| + | |
| − | | + | |
| − | .menu_button.direct_to_page {
| + | |
| − | padding-left: 36px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | .igem_2017_menu_wrapper .menu_button .expand_collapse_icon {
| + | |
| − | width:10%;
| + | |
| − | float:left;
| + | |
| − | }
| + | |
| − | | + | |
| − | .igem_2017_menu_wrapper .menu_button .expand_collapse_icon::before {
| + | |
| − | content: "+";
| + | |
| − | }
| + | |
| − |
| + | |
| − | .open::before {
| + | |
| − | content: "-" !important;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − |
| + | |
| − | /* styling for the menu buttons on hover */
| + | |
| − | .igem_2017_menu_wrapper .menu_button:hover, .igem_2017_menu_wrapper .submenu_button:hover , .submenu_button.current_page:hover {
| + | |
| − | background-color: #6fd700;
| + | |
| − | text-decoration: none;
| + | |
| − | color:#000000;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* styling for the menu button when it is the current page */
| + | |
| − | .current_page {
| + | |
| − | background-color:#7fc1f7 !important;
| + | |
| − | color:#5e5f5f !important;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* styling for the submenu buttons */
| + | |
| − | .igem_2017_menu_wrapper .submenu_button {
| + | |
| − | width: 100%;
| + | |
| − | padding: 10px 0px 10px 34px;
| + | |
| − | float:left;
| + | |
| − | background-color:#ffffff;
| + | |
| − | border-bottom: 1px solid #d3d3d3;
| + | |
| − | font-size: 12px;
| + | |
| − | color: #5e5f5f;
| + | |
| − | cursor: pointer;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* wrapper for the submenu items, they are hidden by default*/
| + | |
| − | .igem_2017_menu_wrapper .submenu_wrapper {
| + | |
| − | display:none;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* when the page size is bigger than 800px, this show/hide control is hidden by default */
| + | |
| − | .igem_2017_menu_wrapper #display_menu_control {
| + | |
| − | display:none;
| + | |
| − | text-align:center;
| + | |
| − | }
| + | |
| − |
| + | |
| − | | + | |
| − | /***************************************************** CONTENT OF THE PAGE ****************************************************/
| + | |
| − | | + | |
| − | /* Wrapper for the content */
| + | |
| − | .igem_2017_content_wrapper {
| + | |
| − | width: 81%;
| + | |
| − | margin: 2%;
| + | |
| − | display:block;
| + | |
| − | float:left;
| + | |
| − | background-color:white;
| + | |
| − | font-family:Tahoma, Geneva, sans-serif;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /********************************* HTML STYLING *********************************/
| + | |
| − | | + | |
| − | /* styling for the titles h1 h2 */
| + | |
| − | .igem_2017_content_wrapper h1, .igem_2017_content_wrapper h2 {
| + | |
| − | padding:5px 15px;
| + | |
| − | border-bottom: 0px;
| + | |
| − | color: #6fd700;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* styling for the titles h3 h4 h5 h6*/
| + | |
| − | .igem_2017_content_wrapper h3, .igem_2017_content_wrapper h4, .igem_2017_content_wrapper h5, .igem_2017_content_wrapper h6 {
| + | |
| − | padding:5px 15px;
| + | |
| − | border-bottom:0px;
| + | |
| − | color: #000000;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* font and text */
| + | |
| − | .igem_2017_content_wrapper p {
| + | |
| − | padding: 0px 15px;
| + | |
| − | font-size: 13px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* Links */
| + | |
| − | .igem_2017_content_wrapper a {
| + | |
| − | font-weight: bold;
| + | |
| − | text-decoration: underline;
| + | |
| − | text-decoration-color: #6fd700;
| + | |
| − | color: #6fd700;
| + | |
| − | -webkit-transition: all 0.4s ease;
| + | |
| − | -moz-transition: all 0.4s ease;
| + | |
| − | -ms-transition: all 0.4s ease;
| + | |
| − | -o-transition: all 0.4s ease;
| + | |
| − | transition: all 0.4s ease;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* hover for the links */
| + | |
| − | .igem_2017_content_wrapper a:hover {
| + | |
| − | text-decoration:none;
| + | |
| − | color:#000000;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* non numbered lists */
| + | |
| − | .igem_2017_content_wrapper ul {
| + | |
| − | padding:0px 20px;
| + | |
| − | font-size: 13px;
| + | |
| − | font-family:Tahoma, Geneva, sans-serif;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* numbered lists */
| + | |
| − | .igem_2017_content_wrapper ol {
| + | |
| − | padding:0px;
| + | |
| − | font-size: 13px;
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| − | font-family:Tahoma, Geneva, sans-serif;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* Table */
| + | |
| − | .igem_2017_content_wrapper table {
| + | |
| − | width: 97%;
| + | |
| − | margin:15px 10px;
| + | |
| − | border: 1px solid #d3d3d3;
| + | |
| − | border-collapse: collapse;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* table cells */
| + | |
| − | .igem_2017_content_wrapper td {
| + | |
| − | padding: 10px;
| + | |
| − | vertical-align: text-top;
| + | |
| − | border: 1px solid #d3d3d3;
| + | |
| − | border-collapse: collapse;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* table headers */
| + | |
| − | .igem_2017_content_wrapper th {
| + | |
| − | padding: 10px;
| + | |
| − | vertical-align: text-top;
| + | |
| − | border: 1px solid #d3d3d3;
| + | |
| − | border-collapse: collapse;
| + | |
| − | background-color:#f2f2f2;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /**********************************LAYOUT CLASSES **********************************/
| + | |
| − | | + | |
| − | /* general class for column divs */
| + | |
| − | .igem_2017_content_wrapper .column {
| + | |
| − | padding: 10px 0px;
| + | |
| − | float:left;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* class for a full width column */
| + | |
| − | .column .full_size {
| + | |
| − | width:100%;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* styling for images in a full width column*/
| + | |
| − | .column.full_size img {
| + | |
| − | width:97%;
| + | |
| − | padding: 10px 15px;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* class for a half width column */
| + | |
| − | .column.half_size {
| + | |
| − | width: 50%;
| + | |
| − | }
| + | |
| − | /* styling for images in a half width column*/
| + | |
| − | .column.half_size img {
| + | |
| − | width: 94.5%;
| + | |
| − | padding: 10px 15px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /********************************* SUPPORT CLASSES ********************************/
| + | |
| − | | + | |
| − | /* class that clears content below*/
| + | |
| − | .igem_2017_content_wrapper .clear {
| + | |
| − | clear:both;
| + | |
| − | }
| + | |
| − |
| + | |
| − |
| + | |
| − | /* adds extra spacing when clearing content */
| + | |
| − | .igem_2017_content_wrapper .clear.extra_space {
| + | |
| − | height: 30px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* highlight class, makes content slightly smaller */
| + | |
| − | .igem_2017_content_wrapper .highlight {
| + | |
| − | margin: 0px 15px;
| + | |
| − | padding: 15px 0px;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* highlight class, adds a gray background */
| + | |
| − | .igem_2017_content_wrapper .highlight.gray {
| + | |
| − | background-color: #f2f2f2;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* highlight with decoration blue line on top */
| + | |
| − | .igem_2017_content_wrapper .highlight.blue_top {
| + | |
| − | border-top: 4px solid #3399ff;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* highlight with a full blue border decoration */
| + | |
| − | .igem_2017_content_wrapper .highlight.blue_border {
| + | |
| − | border: 4px solid #3399ff;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | /* button class */
| + | |
| − | .igem_2017_content_wrapper .button{
| + | |
| − | max-width: 35%;
| + | |
| − | margin: 30px auto;
| + | |
| − | padding: 12px 10px;
| + | |
| − | background-color: #3399ff;
| + | |
| − | text-align: center;
| + | |
| − | color: #ffffff;
| + | |
| − | -webkit-transition: all 0.4s ease;
| + | |
| − | -moz-transition: all 0.4s ease;
| + | |
| − | -ms-transition: all 0.4s ease;
| + | |
| − | -o-transition: all 0.4s ease; transition: all 0.4s ease;
| + | |
| − | }
| + | |
| − | | + | |
| − | /* styling for button on hover */
| + | |
| − | .igem_2017_content_wrapper .button:hover{
| + | |
| − | background-color: #3399ff;
| + | |
| − | color: #000000;
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | /***************************************************** RESPONSIVE STYLING ****************************************************/
| + | |
| − | | + | |
| − | /* IF THE SCREEN IS LESS THAN 1200PX */
| + | |
| − | @media only screen and (max-width: 1200px) {
| + | |
| − | | + | |
| − | #content {width:100%; }
| + | |
| − | .igem_2017_menu_wrapper {width:15%; right:0;}
| + | |
| − | .highlight {padding:10px 0px;}
| + | |
| − | .igem_2017_menu_wrapper #display_menu_control { display:none; }
| + | |
| − | #menu_content { display:block;}
| + | |
| − | .menu_button.direct_to_page {padding-left: 17px;}
| + | |
| − |
| + | |
| − | }
| + | |
| − | | + | |
| − | /* IF THE SCREEN IS LESS THAN 800PX */
| + | |
| − | @media only screen and (max-width: 800px) {
| + | |
| − | | + | |
| − | .igem_2017_menu_wrapper { width:100%; height: 15%; position:relative; left:0%;}
| + | |
| − | .igem_2017_content_wrapper {width:100%; margin-left:0px;}
| + | |
| − | .column.half_size {width:100%; }
| + | |
| − | .column.full_size img, .column.half_size img { width: 100%; padding: 10px 0px;}
| + | |
| − | .highlight {padding:15px 5px;}
| + | |
| − | .igem_2017_menu_wrapper #display_menu_control { display:block; }
| + | |
| − | #menu_content { display:none;}
| + | |
| − | .igem_2017_menu_wrapper .menu_button .expand_collapse_icon { width: 5%; }
| + | |
| − | .menu_bottom_padding {display:none;}
| + | |
| − | .menu_button.direct_to_page { padding-left: 36px; }
| + | |
| − | }
| + | |
| − | | + | |
| − | | + | |
| − |
| + | |
| − |
| + | |
| − | /* special class that the system uses to make sure the team wants a page to be evaluated */
| + | |
| − | .judges-will-not-evaluate {
| + | |
| − | width: 96.6%;
| + | |
| − | margin: 5px 15px;
| + | |
| − | display: block;
| + | |
| − | border: 4px solid #3399ff;
| + | |
| − | font-weight: bold;
| + | |
| − | }
| + | |
| − |
| + | |
| − | </style>
| + | |
| − | | + | |
| − | | + | |
| − | <!--- THIS IS WHERE THE HTML BEGINS --->
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <head>
| + | |
| − | | + | |
| − | <!-- This tells the browser that your page is responsive -->
| + | |
| − | <meta name="viewport" content="width=device-width, initial-scale=1">
| + | |
| − | | + | |
| − | </head>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <!-- start of content -->
| + | |
| − | <div class="igem_2017_content_wrapper">
| + | |
| − | | + | |
| − | <div class="column full_size"> | + | |
| | <img src="https://static.igem.org/mediawiki/2017/4/4e/Matreshki.jpg"> | | <img src="https://static.igem.org/mediawiki/2017/4/4e/Matreshki.jpg"> |
| | </div> | | </div> |
| Line 541: |
Line 10: |
| | | | |
| | <div class="column full_size" > | | <div class="column full_size" > |
| − | <h1> Welcome to Moscow_RF iGEM 2017 team!</h1> | + | <h1> Welcome to Moscow_RF iGEM 2017 team! </h1> |
| − | <p>Wiki is under construction, please stay tuned for updates and check out us on social media (links below)!</p> | + | <p>Wiki is under construction, please stay tuned for updates and check out us on social media (links below)! </p> |
| | </div> | | </div> |
| | | | |
| | <div class="column full_size" > | | <div class="column full_size" > |
| | <h1> Project description </h1> | | <h1> Project description </h1> |
| − | <p>Phytase is an enzyme that can release phosphoric groups from the organic compound known as phytic acid. Phytic acid is of natural origin; it is found in great amounts in grains, beans and some other cultivated plants including forage crops. This compound accounts for most phosphorus in seeds which is vitally important for organisms feeding on grains. However, phytase is not produced in animals’ cells, therefore phosphates contained in feed are not digested effectively and animals have to find other sources of this mineral. This problem is of particular relevance for agricultural industry: manufacturers add phytase to feeds to increase concentration of free phosphates in them for better digestion of this mineral in animals. In the process of granulated compound feed production primary products are subjected to high temperatures destroying most phytase molecules. Our project is aimed at solving these technological problems through using and modifying phytase of Citrobacter braakii. It maintains thermal stability up to 60°С and is able to stand animals’ GIT conditions. To overcome the problem of thermal stability it has been decided to use yeast cells as producers. Yeast cells will serve as capsules for the enzyme protectung it from thermal degradation during feed production. However, using this method results in another problem: phytase is destroyed by specific enzymes in producer cells. In order to avoid this problem our team has decided to modify the protein and attach another protein – phytochelatin – to one of the ends of the protein chain. It has a very stable and compact structure resistant to yeast enzymes. Thus, this protein will become a barrier between phytase and destructive enzymes of yeast cells. Using Yarrowia lipolytica as producers that are able to eliminate toxins will make it possible to recover primary products contaminated by fungi which will reduce production costs. In general, the project is aimed at creating a platform for producing thermally stable enzymes used in agricultural industry.</p> | + | <p>Phytase is an enzyme that can release phosphoric groups from the organic compound known as phytic acid. Phytic acid is of natural origin; it is found in great amounts in grains, beans and some other cultivated plants including forage crops. This compound accounts for most phosphorus in seeds which is vitally important for organisms feeding on grains. However, phytase is not produced in animals’ cells, therefore phosphates contained in feed are not digested effectively and animals have to find other sources of this mineral. This problem is of particular relevance for agricultural industry: manufacturers add phytase to feeds to increase concentration of free phosphates in them for better digestion of this mineral in animals. In the process of granulated compound feed production primary products are subjected to high temperatures destroying most phytase molecules. Our project is aimed at solving these technological problems through using and modifying phytase of Citrobacter braakii. It maintains thermal stability up to 60°С and is able to stand animals’ GIT conditions. To overcome the problem of thermal stability it has been decided to use yeast cells as producers. Yeast cells will serve as capsules for the enzyme protectung it from thermal degradation during feed production. However, using this method results in another problem: phytase is destroyed by specific enzymes in producer cells. In order to avoid this problem our team has decided to modify the protein and attach another protein – phytochelatin – to one of the ends of the protein chain. It has a very stable and compact structure resistant to yeast enzymes. Thus, this protein will become a barrier between phytase and destructive enzymes of yeast cells. Using Yarrowia lipolytica as producers that are able to eliminate toxins will make it possible to recover primary products contaminated by fungi which will reduce production costs. In general, the project is aimed at creating a platform for producing thermally stable enzymes used in agricultural industry.</p> |
| − | </div> | + | </div> |
| | + | |
| | + | <div class="clear"></div> |
| | | | |
| − | <div class="column half_size" > | + | <div class="column full_size" > |
| − | <h5> Moscow_RF on social media </h5> | + | <h5>Moscow_RF on social media: </h5> |
| − | <p> </p>
| + | |
| | <ul> | | <ul> |
| − | <li><a href="https://www.facebook.com/MocowRF.IGEM2017"> @moscow_rf on Facebook</a> </li> | + | <li> <a href="https://www.facebook.com/MocowRF.IGEM2017">@moscow_rf on Facebook</a> </li> |
| − | <li><a href="https://twitter.com/Moscow_RF_iGEM"> @Moscow_RF_iGEM on Twitter</a> </li> | + | <li> <a href="https://twitter.com/Moscow_RF_iGEM">@Moscow_RF_iGEM on Twitter</a></li> |
| − | <li><a href="https://www.instagram.com/moscow_rf_igem_2017/"> @moscow_rf_igem_2017 on Instagram</a> </li> | + | <li> <a href="https://www.instagram.com/moscow_rf_igem_2017/">@moscow_rf_igem_2017 on Instagram</a></li> |
| | </ul> | | </ul> |
| | </div> | | </div> |
| | | | |
| − | <div class="column half_size" >
| |
| − | <h5> About us</h5>
| |
| − | <p></p>
| |
| − | <p></p>
| |
| − | </div>
| |
| − |
| |
| − | <div class="column full_size" >
| |
| − | <h1> About our project </h1>
| |
| − | <p>Wiki is under construction, please stay tuned for updates and check out us on social media (links below)!</p>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − | </p>
| |
| − | <!--
| |
| − | NewPP limit report
| |
| − | CPU time usage: 0.005 seconds
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| − | Real time usage: 0.006 seconds
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| − | Preprocessor visited node count: 9/1000000
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| − | Preprocessor generated node count: 42/1000000
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| − | Post?expand include size: 41/2097152 bytes
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| − | Template argument size: 0/2097152 bytes
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| − | Highest expansion depth: 2/40
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| − | Expensive parser function count: 0/100
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| − | -->
| |
| − |
| |
| − | <!-- Saved in parser cache with key 2017_igem_org:pcache:idhash:9949-0!*!*!*!*!*!* and timestamp 20170828200117 and revision id 81361
| |
| − | -->
| |
| − | </div> <div class="visualClear"></div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − |
| |
| − | <!-- Side Menubar -->
| |
| − | <div id="sideMenu">
| |
| − | <a href="https://2017.igem.org">
| |
| − | <div id="home_logo" >
| |
| − | <img src="https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg">
| |
| − | </div>
| |
| − | </a>
| |
| − |
| |
| − | <div style="clear:both; height:5px;"></div>
| |
| − |
| |
| − | <div id="menuDisplay"></div> <!- Menu will be loaded here ->
| |
| − | </div>
| |
| − |
| |
| − | <!-- Pop_Why Div is definded here -->
| |
| − | <div class="pop_why_cover"></div>
| |
| − |
| |
| − | <div class="pop_why_box" >
| |
| − | <div class="pop_close">? </div>
| |
| − | <div class="pop_why_content"><h3> Loading ... </h3></div>
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| | </html> | | </html> |
