Difference between revisions of "Team:Moscow RF"

 
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<h1> Welcome to Moscow_RF iGEM 2017 team! </h1>
 
<h1> Welcome to Moscow_RF iGEM 2017 team! </h1>
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<p>Wiki is under construction, please stay tuned for updates and check out us on social media (links below)!</p>
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<h1> Project description </h1>
 
<h1> Project description </h1>
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<p>It is well-known that communication and cooperation play important role in our life. Unfortunately, many children, teenagers and adults are deprived of them due to autism.  
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<p>Phytase is an enzyme that can release phosphoric groups from the organic compound known as phytic acid. Phytic acid is of natural origin; it is found in great amounts in grains, beans and some other cultivated plants including forage crops. This compound accounts for most phosphorus in seeds which is vitally important for organisms feeding on grains. However, phytase is not produced in animals’ cells, therefore phosphates contained in feed are not digested effectively and animals have to find other sources of this mineral. This problem is of particular relevance for agricultural industry: manufacturers add phytase to feeds to increase concentration of free phosphates in them for better digestion of this mineral in animals. In the process of granulated compound feed production primary products are subjected to high temperatures destroying most phytase molecules. Our project is aimed at solving these technological problems through using and modifying phytase of Obesumbacterium proteus. It maintains thermal stability up to 60°С and is able to stand animals’ GIT conditions. To overcome the problem of thermal stability it has been decided to use yeast cells as producers. Yeast cells will serve as capsules for the enzyme protectung it from thermal degradation during feed production. However, using this method results in another problem: phytase is destroyed by specific enzymes in producer cells. In order to avoid this problem our team has decided to modify the protein and attach another protein – Cellulose-binding domain – to one of the ends of the protein chain. It has a very stable and compact structure resistant to yeast enzymes. Thus, this protein will become a barrier between phytase and destructive enzymes of yeast cells. Using Yarrowia lipolytica as producers that are able to eliminate toxins will make it possible to recover primary products contaminated by fungi which will reduce production costs. In general, the project is aimed at creating a platform for producing thermally stable enzymes used in agricultural industry.
 
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<p>Autism spectrum disorder (ASD) manifests in brain developmental disturbance that leads to complications in communication, social interaction and stereotyped behavior. Autism has strong impact on individual’s life as well as a society’s life. According to WHO statistics 1 in 160 children had an ASD in 2016. Despite of such prevalence of ASD its pathology and etiology are still unknown and effectiveness of existing therapies is very limited. It has long been presumed that the ASD has a genetic, immune or inflammatory origins, but nowadays scientists notice that environmental factors such as nutrition can also play crucial role in the development of this pathological condition. It was recently reported by many researchers that gluten- and casein-containing food can make the symptoms of ASD more severe.  </p>
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<p>Although gluten/casein-free diets (or GFCF) are very widespread nowadays, just as any diets they have their cons and pros. One the one hand, they may help increasing patient’s quality of life and lighten ASDs symptoms. Such dieting means exception of many useful products that can increase intestinal symptoms of disease. On the other hand, GFCF diets are expensive and in many countries such diet means strong limitation in food diversity. A medicine that can break substances potentially harmful for people with ASD directly in their organisms may solve this problem. Such medicine will allow patients to stick to “normal” diet instead of GFCF diet.
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The aim of our project is to make a biological system that will break down gluten and casein in individual’s gastro-intestinal tract. We plan using E. coli bacterium as a chassis organism for our project. Our modified E. coli strain will bear human dipeptidyl peptidase IV gene, a gene encoding enzyme that breaks down gluten. One of the challenges of our project is making DDP IV express and work only in GIT and nowhere else. Another one is making such design that will allow expression of DDP IV only in presence of gluten/casein. All of this will help avoiding potential side effects and making use of such medicine safe. </p>
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<h5>What is phytase?</h5>
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<h5> Moscow_RF on social media </h5>
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<h5>Moscow_RF on social media: </h5>
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<li><a href="https://twitter.com/Moscow_RF_iGEM"> @Moscow_RF_iGEM on Twitter</a> </li>
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<li> <a href="https://www.facebook.com/MocowRF.IGEM2017">@moscow_rf on Facebook</a> </li>
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<li><a href="https://www.instagram.com/moscow_rf_igem_2017/"> @moscow_rf_igem_2017 on Instagram</a> </li>
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<li> <a href="https://twitter.com/Moscow_RF_iGEM">@Moscow_RF_iGEM on Twitter</a></li>
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<li> <a href="https://www.instagram.com/moscow_rf_igem_2017/">@moscow_rf_igem_2017 on Instagram</a></li>
 
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<h5> About us</h5>
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<h1> About our project </h1>
 
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<p>Wiki is under construction, please stay tuned for updates and check out us on social media (links below)!</p>
 
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<h1> Team members</h1>
 
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<p>Wiki is under construction, please stay tuned for updates and check out us on social media (links below)!</p>
 
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Latest revision as of 14:22, 30 October 2017

HOME
TEAM
PROJECT DESIGN
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Demonstrate
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PARTS
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Basic Parts
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SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
JUDGING FORM
THANKS

Welcome to Moscow_RF iGEM 2017 team!

Project description

Phytase is an enzyme that can release phosphoric groups from the organic compound known as phytic acid. Phytic acid is of natural origin; it is found in great amounts in grains, beans and some other cultivated plants including forage crops. This compound accounts for most phosphorus in seeds which is vitally important for organisms feeding on grains. However, phytase is not produced in animals’ cells, therefore phosphates contained in feed are not digested effectively and animals have to find other sources of this mineral. This problem is of particular relevance for agricultural industry: manufacturers add phytase to feeds to increase concentration of free phosphates in them for better digestion of this mineral in animals. In the process of granulated compound feed production primary products are subjected to high temperatures destroying most phytase molecules. Our project is aimed at solving these technological problems through using and modifying phytase of Obesumbacterium proteus. It maintains thermal stability up to 60°С and is able to stand animals’ GIT conditions. To overcome the problem of thermal stability it has been decided to use yeast cells as producers. Yeast cells will serve as capsules for the enzyme protectung it from thermal degradation during feed production. However, using this method results in another problem: phytase is destroyed by specific enzymes in producer cells. In order to avoid this problem our team has decided to modify the protein and attach another protein – Cellulose-binding domain – to one of the ends of the protein chain. It has a very stable and compact structure resistant to yeast enzymes. Thus, this protein will become a barrier between phytase and destructive enzymes of yeast cells. Using Yarrowia lipolytica as producers that are able to eliminate toxins will make it possible to recover primary products contaminated by fungi which will reduce production costs. In general, the project is aimed at creating a platform for producing thermally stable enzymes used in agricultural industry.

What is phytase?

Moscow_RF on social media: