Difference between revisions of "Team:AshesiGhana/Parts"

We would like to apologize for the issue of the parts being received on time but not being accepted, due to mislabeling of the package. At the time of shipment, the package was open, and the shipper has seen the contents and was told that contains DNA samples in a plate sample holder. However only the specification of the plate sample holder was included on the shipping documents. We take full responsibility for not being more diligent and checking all the shipping requirements. In an effort to correct, an additional sample has been labeled properly and has been shipped.

We humbly request that you would consider the miscommunication with the shipper and will still accept our submission for the medal criteria. Thank you for your consideration and time.

Parts

NowGFP-golB (FRET DONOR) This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.

High Potential Iron Sulphur Protein (HiPiP)This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.

pH resistance EcoliThis part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment

pH resistance EcoliThis part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.

@@ Line 1: / Line 1: @@
 {{Template:AshesiGhana/NavBar}}
+{{Template:AshesiGhana/NavBar2}}
-<html lang="en-GB">
+{{ManchesterMobile}}
+<html>
 <head>
-    <link href='//fonts.googleapis.com/css?family=Raleway%7CMerriweather%7CPT+Mono' rel='stylesheet' type='text/css'>
-    <style>
+<style>
-img {
- vertical-align:middle
-}
-fieldset {
- border:0;
- margin:0;
- padding:0
-}
-textarea {
- resize:vertical
-}
-::-moz-selection {
- background:#C8E6FD;
- text-shadow:none
-}
-::selection {
- background:#C8E6FD;
- text-shadow:none
-}
 body {
- background:#fff;
+    background-color:;
- font:1em/2 Merriweather,Georgia,Times,serif;
+    overflow-x: hidden
- color:#555
 }
-a {
- color:#494B4B;
+.sectionfirst {
- border-bottom:1px solid #02BCDC;
+  margin-top: 25px!important;
- -o-transition:.25s;
+  padding-top: 10vh;
- -ms-transition:.25s;
+  height: 30vh;
- -moz-transition:.25s;
+  background: transparent;
- -webkit-transition:.25s;
+  background-size: cover
- transition:.25s
 }
-a:hover {
- color:#02BCDC
+.border {
+  background-color: #c4c4c4;
+  color: #fff;
+  padding: 18px;
+  letter-spacing: 10px;
+  opacity: 1
 }
-h1,h2,h3,h4,h5,h6 {
- font-weight:400;
+.col-md-4 img {
- font-family:Raleway,Arial,sans-serif;
+  width: 20vw;
- line-height:1.5em;
+  display: block;
- color:#666
+  margin-left: auto;
+  margin-right: auto
 }
-.header-container {
- margin:1em 1em 0
+.displaybox {
+    padding-top: 10px;
+    display: block;
+    min-height: 40vh;
+    border: 3px solid transparent;
+    border-radius:;
+    width: 100vw!important;
+    overflow-x: hidden;
+    opacity: 1.0;
+    -webkit-transition: 0.3s ease-in-out;
+    -moz-transition: 0.3s ease-in-out;
+    -ms-transition: 0.3s ease-in-out;
+    -o-transition: 0.3s ease-in-out;
+    transition: 0.3s ease-in-out
 }
-.title a {
- width:181px;
+h4 {
- height:51px;
+  font-size: 35px!important;
- border:0;
+  font-weight: 400;
- text-indent:-9999px;
+  font-family: 'Calibri'!important;
- display:block;
+  padding: 2%!important;
- background-image:url(../img/site-name.png);
+  text-align: center;
- background-image:-webkit-image-set(url(../img/site-name.png) 1x,url(../img/site-name-2x.png) 2x);
- background-position:left center;
- background-repeat:no-repeat
 }
-.main-container {
+h2 {
- margin:0 1em 2em
+  font-size: 40px!important;
+  font-weight: 400;
+  font-family: 'Calibri'!important;
+  padding: 2%!important;
+  text-align: center;
 }
-code,pre {
- font-family:"PT Mono","Courier New",Courier,monospace;
+h3 {
- color:#D50085
+  font-size: 20px!important;
+  padding: 2%!important;
+  background: #89d4fa!important;
+  color: #111;
+  border: 1px solid #44bfff;
+  border-radius: .25em;
+  text-align: justify;
+  text-justify: inter-word;
 }
-pre {
- line-height:1.5em;
- margin:1.1em 0
-}
-article:not(:last-child) {
- margin-bottom:2em
-}
-.meta {
- background:#EDF0F3;
- padding:.5em 1em
-}
-.meta h4,.meta p {
- display:table-cell;
- line-height:2em;
- margin:0
-}
-.meta h4 {
- float:left;
- margin-right:.5em
-}
-.comments div {
- background:#E9F5F8;
- padding:.5em 1em;
- margin:1em 0;
- word-wrap:break-word
-}
-.comments div:nth-child(odd) {
- background:url(../img/comment-left-bg.png)bottom left no-repeat #E2F2FE;
- padding-left:2em
-}
-.comments div:nth-child(even) {
- background:url(../img/comment-right-bg.png)bottom right no-repeat #E2F2FE;
- padding-right:2em
-}
-.comments h4,.comments p {
- margin:.5em 0
-}
-.comments p {
- line-height:1.5em
-}
-.comments h4 {
- line-height:1em;
- margin-bottom:1em
-}
-.footer-container {
- margin:1em
-}
-.footer-container footer {
- padding-top:1em;
- border-top:1px dashed #70828F
-}
-.footer-container ul {
- list-style-type:none;
- padding-left:0
-}
-.footer-container li {
- display:inline
-}
-.footer-container a {
- display:inline-block;
- background:#366485;
- color:#eee;
- border:0;
- padding:.3em .5em;
- margin-bottom:.3em;
- line-height:1.2em
-}
-.footer-container a:hover {
- background:#4089BF
-}
-@media only screen and (min-width:620px) {
- .header-container {
-  margin:2em 2em 0
- }
- .header-container .title {
-  float:left
- }
-</style>
+p {
-<style type="text/css">
+  text-align: justify;
-    /*! normalize.css v3.0.2 | MIT License | git.io/normalize */html {
+  text-justify: inter-word;
- font-family:sans-serif;
+  font-family: 'Calibri', sans-serif;
- -ms-text-size-adjust:100%;
- -webkit-text-size-adjust:100%
-}
-body {
- margin:0
-}
-article,aside,details,figcaption,figure,footer,header,hgroup,main,menu,section,summary {
- display:block
-}
-audio,canvas,progress,video {
- display:inline-block;
- vertical-align:baseline
-}
-audio:not([controls]) {
- display:none;
- height:0
-}
-[hidden],template {
- display:none
-}
-a {
- background-color:transparent
-}
-a:active,a:hover {
- outline:0
-}
-abbr[title] {
- border-bottom:1px dotted
-}
-b,strong {
- font-weight:700
-}
-dfn {
- font-style:italic
-}
-h1 {
- font-size:2em;
- margin:.67em 0
-}
-mark {
- background:#ff0;
- color:#000
-}
-small {
- font-size:80%
-}
-sub,sup {
- font-size:75%;
- line-height:0;
- position:relative;
- vertical-align:baseline
-}
-sup {
- top:-.5em
-}
-sub {
- bottom:-.25em
-}
-img {
- border:0
-}
-svg:not(:root) {
- overflow:hidden
-}
-figure {
- margin:1em 40px
-}
-hr {
- -moz-box-sizing:content-box;
- box-sizing:content-box;
- height:0
-}
-pre {
- overflow:auto
-}
-code,kbd,pre,samp {
- font-family:monospace,monospace;
- font-size:1em
-}
-button,input,optgroup,select,textarea {
- color:inherit;
- font:inherit;
- margin:0
-}
-button {
- overflow:visible
-}
-button,select {
- text-transform:none
-}
-button,html input[type=button],input[type=reset],input[type=submit] {
- -webkit-appearance:button;
- cursor:pointer
-}
-button[disabled],html input[disabled] {
- cursor:default
-}
-button::-moz-focus-inner,input::-moz-focus-inner {
- border:0;
- padding:0
-}
-input {
- line-height:normal
-}
-input[type=checkbox],input[type=radio] {
- box-sizing:border-box;
- padding:0
-}
-input[type=number]::-webkit-inner-spin-button,input[type=number]::-webkit-outer-spin-button {
- height:auto
-}
-input[type=search] {
- -webkit-appearance:textfield;
- -moz-box-sizing:content-box;
- -webkit-box-sizing:content-box;
- box-sizing:content-box
-}
-input[type=search]::-webkit-search-cancel-button,input[type=search]::-webkit-search-decoration {
- -webkit-appearance:none
-}
-fieldset {
- border:1px solid silver;
- margin:0 2px;
- padding:.35em .625em .75em
-}
-legend {
- border:0;
- padding:0
-}
-textarea {
- overflow:auto
-}
-optgroup {
- font-weight:700
-}
-table {
- border-collapse:collapse;
- border-spacing:0
-}
-td,th {
- padding:0
 }
-</style>
-    <meta charset="utf-8">
-    <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
-    <meta name="description" content="Rob Bell's software development blog, discussing object-oriented programming, design and best practices, amongst other things.">
-    <meta name="viewport" content="width=device-width, initial-scale=1.0">
-        <title>
-    A beginner&#39;s guide to Big O notation
- - Rob Bell</title>
-    <link rel="apple-touch-icon" href="apple-touch-icon.png">
-</head>
+.box {
-<body>
+  width: 80%;
-    <div class="header-container clearfix">
+  border: 2px solid #000;
-        <header>
+  margin: 150px auto 15px;
-            <h2 class="title"><a href="/">Rob Bell</a></h2>
+  text-align: center;
-            <nav>
+  padding: 20px;
-                <ul>
+  font-weight: bold;
-                    <li><a href="#1">FRET Donor</a></li>
+  border-radius: 10px;
-                    <li><a href="#2">FRET Acceptor</a></li>
-                    <li><a href="#3">Enzyme Part 00k1</a></li>
-                    <li><a href="#4">pH resistance Ecoli</a></li>
-                    <li><a href="#5">HIPIP Ecoli</a></li>
-                </ul>
-            </nav>
-        </header>
-    </div>
-    <div class="main-container">
+}
-        <div class="main clearfix">
-<article>
+.info {
-    <header>
+  background-color: #ddd;
+  border-color: #aaa;
+}
+.warning {
+  background-color: #FFF484;
+  border-color: #DCC600;
+}
-                    <h1>Parts</h1>
+.success {
+  background-color: #B9FFAB;
+  border-color: #116400;
+  color: #116400;
+}
+.error {
+  background-color: #EBB1B1;
+  border-color: #973939;
+  color: #973939;
+}
+</style>
+</head>
-                    <p></p>
+<body>
+<div class="info box">
+ <p> We would like to apologize for the issue of the parts being received on time but not being accepted, due to mislabeling of the package. At the time of shipment, the package was open, and the shipper has seen the contents and was told that contains DNA samples in a plate sample holder. However only the specification of the plate sample holder was included on the shipping documents. We take full responsibility for not being more diligent and checking all the shipping requirements. In an effort to correct, an additional sample has been labeled properly and has been shipped. </p>
+<p>We humbly request that you would consider the miscommunication with the shipper and will still accept our submission for the medal criteria. Thank you for your consideration and time.</p>
-                </header>
+</div>
+<div class="clear"></div>
+<div class="col-md-12 sectionfirst" style="background-color: #fbf9f8!important">
+<h2 class="border" style="margin-top: 5vh; text-align: center">Parts</h2>
+<br/>
+</div>
-                <section>
+<div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important">
-                    <p></p>
+<center>
+<img src= "https://static.igem.org/mediawiki/2017/4/4a/T--AshesiGhana--fretdonor.png">
+<br>
+<br>
+<p><font color="#111"><center></h3><b>NowGFP-golB (FRET DONOR)</b> This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.
+<br>
+<br>
+<br>
+<br>
+<img src="https://static.igem.org/mediawiki/2017/4/4f/T--AshesiGhana--fretacceptor.png">
+<br>
+<br>
+<p><font color="#111"><center></h3><b>High Potential Iron Sulphur Protein (HiPiP)</b>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.
+<br>
+<br>
+<br>
+<br>
+<img src="https://static.igem.org/mediawiki/2017/4/47/T--AshesiGhana--phresistecoli.png">
+<br>
+<br>
+<p><font color="#111"><center></h3><b>pH resistance Ecoli</b>This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment
+<br><br>
+<br>
+<br>
+<img src="https://static.igem.org/mediawiki/2017/1/1a/T--AshesiGhana--hipipecoli.png">
+<br>
+<br>
+<p><font color="#111"><center></h3><b>pH resistance Ecoli</b>This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.
+<br>
+<br></div>
+</div>
-                    <p></p>
-                </section>
-                <section id="1">
-                    <h2>NowGFP-golB (FRET DONOR)</h2>
-                    <img src="https://static.igem.org/mediawiki/2017/4/4a/T--AshesiGhana--fretdonor.png" alt="Mountain View" >
-                    <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
-                    <pre></pre>
-                </section>
-                <section id="2">
-                   <h2>mRuby2-golB (FRET ACCEPTOR)</h2>
-                    <img src="https://static.igem.org/mediawiki/2017/4/4f/T--AshesiGhana--fretacceptor.png" alt="Mountain View" >
-                    <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
-                    <pre></pre>
-                </section>
-                <section id="3">
-                    <h2>Enzyme Part 00k1</h2>
-                    <img src="https://static.igem.org/mediawiki/2017/5/55/T--AshesiGhana--enzymepart1.png" alt="Mountain View" >
-                    <p>To aid in the gold liberation process this part has a Tetrathionate Hydrolase gene which was mimicked from the Acidithiobacillus ferrooxidans into the E.coli strain, to function in sulphur and iron oxidizing, a process used by the Acidithiobacillus ferrooxidans in the process of gold liberation. With its own constitutive promoter, RBS and terminator this part functions as one important part in the gold liberation goal of this project.</p>
-                    <pre></pre>
-                <section id="4">
-                      <h2> pH resistance Ecoli</h2>
-                    <img src="https://static.igem.org/mediawiki/2017/4/47/T--AshesiGhana--phresistecoli.png" alt="Mountain View" >
-                    <p>This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment</p>
-                    <pre></pre>
-                </section>
-                <section id="5">
-                      <h2>HIPIP Ecoli</h2>
-                    <img src="https://static.igem.org/mediawiki/2017/1/1a/T--AshesiGhana--hipipecoli.png" alt="Mountain View" >
-                    <p>This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.</p>
-                    <pre></pre>
-                </section>
-</article>
-        </div>
-    </div>
 </body>
 </html>
 {{Template:AshesiGhana/Footer}}