Team:HFLS H2Z Hangzhou/sub/parts

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Team Members

Jianan Li
Qingrui Sun
Yiming Rong
Jiayue Guo
Zhiyuan Lu
Meiqi Yuan
Zhengyao Lin
Caiyi Feng
Shuyun Zhang
Tenghao Huang
Yining Huang
Yanyue Zhu

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Parts

Basic Parts

BBa_K2346000 Profile

Nitric oxide reductase (NOR)

Coding sequence for enzyme that will catalyst the reduction of NO(nitrogen monoxide) to N2O (nitrous oxide).

BBa_K2346001 Profile

Nitrite reductase (NiR)

Coding sequence for enzyme that will catalyst the reduction of Nitrite ion (NO2-) to NO(nitrogen monoxide).

BBa_K2346002 Profile

Nitrous oxide reductase (nosZ)

Coding sequence for enzyme that will catalyst the reduction of N2O(nitrous oxide) to Nitrogen and Oxygen.

BBa_K2346009 Profile

GGGGS x2 Linker

Coding sequence for a flexible linker. It has advantages like improving protein solubility, providing enzyme flexibility for catalysis, domain separation.

BBa_K2346010 Profile

EAAAK x2 linker

Coding sequence for an α-helix forming linker, has advantages of improving protein solubility, providing enzyme flexibility for catalysis Domain separation.

BBa_K2346011 Profile

Nitrite Degradation Fusion Enzyme(NzFE)

Coding sequence for a fusion enzyme made from nosZ, NiR, NOR. The start codons of NiR and NOR are eliminated during PCR stage of inFusion Assembly. The end codons of NiR and nosZ are eliminated during PCR stage of inFusion Assembly.