Difference between revisions of "Team:AshesiGhana/Parts"

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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<p> We would like to apologize for the issue of the parts being received on time but not being accepted, due to mislabeling of the package. At the time of shipment, the package was open, and the shipper has seen the contents and was told that contains DNA samples in a plate sample holder. However only the specification of the plate sample holder was included on the shipping documents. We take full responsibility for not being more diligent and checking all the shipping requirements. In an effort to correct, an additional sample has been labeled properly and has been shipped. </p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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<p>We humbly request that you would consider the miscommunication with the shipper and will still accept our submission for the medal criteria. Thank you for your consideration and time.</p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h2 class="border" style="margin-top: 5vh; text-align: center">Parts</h2>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
 
  
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<p><font color="#111"><center></h3><b>NowGFP-golB (FRET DONOR)</b> This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.
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<p><font color="#111"><center></h3><b>High Potential Iron Sulphur Protein (HiPiP)</b>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.
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<p><font color="#111"><center></h3><b>pH resistance Ecoli</b>This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment
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<p><font color="#111"><center></h3><b>pH resistance Ecoli</b>This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.
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<groupparts>iGEM17 AshesiGhana</groupparts>
 
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{{Template:AshesiGhana/Footer}}

Latest revision as of 02:00, 2 November 2017

Ashesi University
College
HOME
TEAM
PROJECT
Overview
Description
Results
Demonstration
Medal Criteria
Achievement
NOTEBOOK
Protocols
Diary
Lab Book
InterLab
Measurement
PARTS
Parts
Basic Parts
Composite Parts
Improved Parts
Part Collection
HUMAN PRACTICES
Silver HP
Water Industry
Integrated and Gold
Intellectual Property
Public Engagement
Entrepreneurship
MODELLING
Overview
D. O. Experiment
Continuous Culture
PHO Operon
JUDGING FORM

We would like to apologize for the issue of the parts being received on time but not being accepted, due to mislabeling of the package. At the time of shipment, the package was open, and the shipper has seen the contents and was told that contains DNA samples in a plate sample holder. However only the specification of the plate sample holder was included on the shipping documents. We take full responsibility for not being more diligent and checking all the shipping requirements. In an effort to correct, an additional sample has been labeled properly and has been shipped.

We humbly request that you would consider the miscommunication with the shipper and will still accept our submission for the medal criteria. Thank you for your consideration and time.

Parts




NowGFP-golB (FRET DONOR) This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.





High Potential Iron Sulphur Protein (HiPiP)This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.





pH resistance EcoliThis part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment





pH resistance EcoliThis part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.