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| | </style> | | </style> |
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| − | <meta charset="utf-8">
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| − | <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
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| − | <meta name="description" content="Rob Bell's software development blog, discussing object-oriented programming, design and best practices, amongst other things.">
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| − | <meta name="viewport" content="width=device-width, initial-scale=1.0">
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| − | <title>
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| − | A beginner's guide to Big O notation
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| − | - Rob Bell</title>
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| − | <link rel="apple-touch-icon" href="apple-touch-icon.png">
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| | | | |
| | </head> | | </head> |
| | + | |
| | <body> | | <body> |
| − | <div class="header-container clearfix">
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| − | <header>
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| − | <h2 class="title"><a href="/">Rob Bell</a></h2>
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| − | <nav>
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| − | <ul>
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| − | <li><a href="#1">FRET Donor</a></li>
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| − | <li><a href="#2">FRET Acceptor</a></li>
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| − | <li><a href="#3">Enzyme Part 00k1</a></li>
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| − | <li><a href="#4">pH resistance Ecoli</a></li>
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| − | <li><a href="#5">HIPIP Ecoli</a></li>
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| − | </ul>
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| − | </nav>
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| − | </header>
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| − | </div>
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| − | <div class=" main-container"> | + | <div class="clear"></div> |
| − | <div class="main clearfix"> | + | |
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| − | <article> | + | <div class="col-md-12 sectionfirst" style="background-color: #fbf9f8!important"> |
| − | <header>
| + | <h2 class="border" style="margin-top: 5vh; text-align: center">Parts</h2> |
| | + | <br/> |
| | + | </div> |
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| − | <h1>Parts</h1>
| + | <div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important"> |
| | + | <center> |
| | + | <h4><font color="#111">NowGFP-golB (FRET DONOR)</font></h4> |
| | + | <hr class="dark"> |
| | + | </center> |
| | | | |
| | + | <center> |
| | + | <img src= "https://static.igem.org/mediawiki/2017/4/4a/T--AshesiGhana--fretdonor.png"> |
| | + | <br> |
| | + | <br> |
| | + | <p><font color="#111"><center></h3><b>NowGFP-golB (FRET DONOR)</b> This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins. |
| | + | <br> |
| | + | <br> |
| | + | <br> |
| | + | <br> |
| | + | <img src="https://static.igem.org/mediawiki/2017/4/4f/T--AshesiGhana--fretacceptor.png"> |
| | + | <br> |
| | + | <br> |
| | + | <p><font color="#111"><center></h3><b>High Potential Iron Sulphur Protein (HiPiP)</b>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins. |
| | + | <br> |
| | + | <br> |
| | + | <br> |
| | + | <br> |
| | + | <img src="https://static.igem.org/mediawiki/2017/4/47/T--AshesiGhana--phresistecoli.png"> |
| | + | <br> |
| | + | <br> |
| | + | <p><font color="#111"><center></h3><b>pH resistance Ecoli</b>This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment |
| | + | <br><br> |
| | + | <br> |
| | + | <br> |
| | + | <img src="https://static.igem.org/mediawiki/2017/1/1a/T--AshesiGhana--hipipecoli.png"> |
| | + | <br> |
| | + | <br> |
| | + | <p><font color="#111"><center></h3><b>pH resistance Ecoli</b>This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal. |
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| | + | <br></div> |
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| | + | </div> |
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| − | </section>
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| − | <section id="1">
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| − | <h2>NowGFP-golB (FRET DONOR)</h2>
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| − | <img src="https://static.igem.org/mediawiki/2017/4/4a/T--AshesiGhana--fretdonor.png" alt="Mountain View" >
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| − |
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| − | <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
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| − | <pre></pre>
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| − | </section>
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| − | <section id="2">
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| − | <h2>mRuby2-golB (FRET ACCEPTOR)</h2>
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| − | <img src="https://static.igem.org/mediawiki/2017/4/4f/T--AshesiGhana--fretacceptor.png" alt="Mountain View" >
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| − |
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| − | <p>This part is a composite part composed of a green fluorescent protein (NowGFP) and a golB protein along with its own constitutive promoter, RBS and terminator with a spacer between the two genes. This composite part serves as the donor part of the FRET mechanism employed in this project. With the golB binding to gold atoms and the NowGFP giving off fluorescence to excite acceptor proteins.</p>
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| − | </section>
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| − | <section id="3">
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| − | <h2>Enzyme Part 00k1</h2>
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| − | <img src="https://static.igem.org/mediawiki/2017/5/55/T--AshesiGhana--enzymepart1.png" alt="Mountain View" >
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| − |
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| − | <p>To aid in the gold liberation process this part has a Tetrathionate Hydrolase gene which was mimicked from the Acidithiobacillus ferrooxidans into the E.coli strain, to function in sulphur and iron oxidizing, a process used by the Acidithiobacillus ferrooxidans in the process of gold liberation. With its own constitutive promoter, RBS and terminator this part functions as one important part in the gold liberation goal of this project.</p>
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| − | <pre></pre>
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| − | <h2> pH resistance Ecoli</h2>
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| − | <img src="https://static.igem.org/mediawiki/2017/4/47/T--AshesiGhana--phresistecoli.png" alt="Mountain View" >
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| − | <p>This part is a composite part composed of a pH_resistance gene. With its own constitutive promoter, RBS and a double terminator, the part is designed for the E.coli to provide pH resistance to enable it to survive in the bio-mining environment</p>
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| − | <pre></pre>
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| − | <h2>HIPIP Ecoli</h2>
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| − | <img src="https://static.igem.org/mediawiki/2017/1/1a/T--AshesiGhana--hipipecoli.png" alt="Mountain View" >
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| − | <p>This part is composed of a High potential iron-sulphur protein (HiPIP) with its own constitutive Promoter, RBS and double terminator. This part in addition to the Tetrathionate Hydrolase part enable the bacteria (E.coli) to liberate the gold from its ore similar to the way the Acidithiobacillus Ferrooxidans achieves this goal.</p>
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