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+ | {{Template:AshesiGhana/NavBar}} | ||
+ | {{Template:AshesiGhana/NavBar2}} | ||
+ | {{ManchesterMobile}} | ||
+ | <html> | ||
+ | <head> | ||
+ | <style> | ||
+ | |||
+ | body { | ||
+ | background-color:; | ||
+ | overflow-x: hidden | ||
+ | } | ||
+ | |||
+ | .sectionfirst { | ||
+ | margin-top: 25px!important; | ||
+ | padding-top: 10vh; | ||
+ | height: 30vh; | ||
+ | background: transparent; | ||
+ | background-size: cover | ||
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+ | |||
+ | .border { | ||
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+ | color: #fff; | ||
+ | padding: 18px; | ||
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+ | } | ||
+ | |||
+ | .col-md-4 img { | ||
+ | width: 20vw; | ||
+ | display: block; | ||
+ | margin-left: auto; | ||
+ | margin-right: auto | ||
+ | } | ||
+ | |||
+ | .displaybox { | ||
+ | padding-top: 10px; | ||
+ | display: block; | ||
+ | min-height: 40vh; | ||
+ | border: 3px solid transparent; | ||
+ | border-radius:; | ||
+ | width: 100vw!important; | ||
+ | overflow-x: hidden; | ||
+ | opacity: 1.0; | ||
+ | -webkit-transition: 0.3s ease-in-out; | ||
+ | -moz-transition: 0.3s ease-in-out; | ||
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+ | transition: 0.3s ease-in-out | ||
+ | } | ||
+ | |||
+ | h4 { | ||
+ | font-size: 35px!important; | ||
+ | font-weight: 400; | ||
+ | font-family: 'Calibri'!important; | ||
+ | padding: 2%!important; | ||
+ | text-align: center; | ||
+ | } | ||
+ | |||
+ | h2 { | ||
+ | font-size: 40px!important; | ||
+ | font-weight: 400; | ||
+ | font-family: 'Calibri'!important; | ||
+ | padding: 2%!important; | ||
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+ | } | ||
+ | |||
+ | h3 { | ||
+ | font-size: 20px!important; | ||
+ | padding: 2%!important; | ||
+ | background: #89d4fa!important; | ||
+ | color: #111; | ||
+ | border: 1px solid #44bfff; | ||
+ | border-radius: .25em; | ||
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+ | font-family: 'Calibri', sans-serif; | ||
+ | } | ||
+ | |||
+ | </style> | ||
+ | |||
+ | </head> | ||
+ | |||
+ | <body> | ||
+ | |||
+ | <div class="col-md-12 sectionfirst" style="background-color: #fbf9f8!important"> | ||
+ | <h2 class="border" style="margin-top: 5vh; text-align: center">Results</h2> | ||
+ | <br/> | ||
+ | </div> | ||
+ | |||
+ | <div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important"> | ||
+ | <center> | ||
+ | <h4><font color="#111">Part Synthesis Results</font></h4> | ||
+ | <hr class="dark"> | ||
+ | </center> | ||
+ | |||
+ | <p><b>ACIDITHIOBACILLUS FEROXIDANS DESIGN PARTS</b></p> | ||
+ | <table class="table table-bordered table-striped"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Part</th> | ||
+ | <th>Synthesis Status</th> | ||
+ | |||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>ORIGIN OF REPLICATION <img src="https://static.igem.org/mediawiki/2017/5/52/T--AshesiGhana--orep.png"></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>FRET-DONOR <img src="https://static.igem.org/mediawiki/2017/6/6a/T--AshesiGhana--fretdon.png" ></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>FRET-ACCEPTOR<img src="https://static.igem.org/mediawiki/2017/9/9b/T--AshesiGhana--fretacc.png"></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | |||
+ | <p><b>ECOLI DESIGN PARTS</b></p> | ||
+ | <table class="table table-bordered table-striped"> | ||
+ | <thead> | ||
+ | <tr> | ||
+ | <th>Part</th> | ||
+ | <th>Synthesis Status</th> | ||
+ | |||
+ | </tr> | ||
+ | </thead> | ||
+ | <tbody> | ||
+ | |||
+ | <tr> | ||
+ | <td>FRET-DONOR <img src="https://static.igem.org/mediawiki/2017/6/6a/T--AshesiGhana--fretdon.png" ></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>FRET-ACCEPTOR<img src="https://static.igem.org/mediawiki/2017/9/9b/T--AshesiGhana--fretacc.png"></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>HIGH POTENTIAL IRON SULFUR PROTEIN (HIPIP)<img src="https://static.igem.org/mediawiki/2017/7/75/T--AshesiGhana--hipip.png"></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>PH RESISTANCE<img src="https://static.igem.org/mediawiki/2017/8/8e/T--AshesiGhana--hpresist.png"></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>TETRATHIONATE HYDROLASE<img src="https://static.igem.org/mediawiki/2017/3/36/T--AshesiGhana--tetrahydro.png"></td> | ||
+ | <td>Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>MRUBY 2<img src="https://static.igem.org/mediawiki/2017/3/36/T--AshesiGhana--tetrahydro.png"></td> | ||
+ | <td>Not Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>NowGFP<img src="https://static.igem.org/mediawiki/2017/3/36/T--AshesiGhana--tetrahydro.png"></td> | ||
+ | <td>Not Synthesized Successfully</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important"> | ||
+ | <center> | ||
+ | <h4><font color="#111">PARTS DIGESTED AND TRANSFORMED</font></h4> | ||
+ | <hr class="dark"> | ||
+ | </center> | ||
+ | |||
+ | <p><font color="#111"><br> | ||
+ | |||
+ | <ol style="font-size:16px;"> | ||
+ | |||
+ | <li><b>Make up ligation reaction as below</b><br></li> | ||
+ | <li><b>High Potential Iron Sulphur Protein (HiPiP)</b><br></li> | ||
+ | <li><b>Tetrathionate Hydrolase</b><br></li> | ||
+ | <li><b>Fret-Donor </b><br></li> | ||
+ | <li><b>Fret-Acceptor</b><br></li> | ||
+ | </ol> | ||
+ | <br> | ||
+ | <br> | ||
+ | <b>Complete Total Number of Transformations = 5</b><br> | ||
+ | <b>Successful number of Transformations = 2</b><br> | ||
+ | <b>Number of failed Transformations = 3</b><br> | ||
+ | </font></p> | ||
+ | </div> | ||
+ | <br> | ||
+ | <br> | ||
+ | |||
+ | |||
+ | <div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important"> | ||
+ | <center> | ||
+ | <h4><font color="#111">Results pH Resistance Gene</font></h4> | ||
+ | <hr class="dark"> | ||
+ | </center> | ||
+ | |||
+ | <p><font color="#111"><b>Total number of Transformations = 3</b><br> | ||
+ | <b>Successful number of Transformations = 2</b><br> | ||
+ | <b>Number of failed transformations = 1</b><br> | ||
+ | </font></p> | ||
+ | |||
+ | <p><font color="#111"><b>Total number of gels runs = 3</b><br> | ||
+ | <b>Successful number of gel runs = 1</b><br> | ||
+ | <b>Number of failed gel runs = 2</b><br> | ||
+ | </font></p> | ||
+ | <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f8/T--AshesiGhana--gelimage.jpg"> | ||
+ | <br> | ||
+ | <p><font color="#111">From the gel image in figure 1 above it is evident that the pH resistance gene was inserted in the plasmid, however due to a small concentration of the DNA present, the band of the insert seemed to be blurred out. | ||
+ | </font></p> | ||
+ | |||
+ | <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/a/aa/T--AshesiGhana--effgraph.jpg"> | ||
+ | <br> | ||
+ | <p><font color="#111">From the results obtained and displayed in the graph above, it is evident that the pH resistance gene is effective for pH levels ranging from 5.5 to 7.5, where 7.5 is the pH level of the original media for the bacterial growth. It is also observed that, the resistance gene was not effective with pH levels 4.5 and below, resulting in no bacterial growth over time. | ||
+ | </font></p> | ||
+ | </div> | ||
+ | |||
+ | </body> | ||
+ | </html> | ||
+ | {{Template:AshesiGhana/Footer}} |
Revision as of 00:27, 1 November 2017
Results
Part Synthesis Results
ACIDITHIOBACILLUS FEROXIDANS DESIGN PARTS
Part | Synthesis Status |
---|---|
ORIGIN OF REPLICATION | Synthesized Successfully |
FRET-DONOR | Synthesized Successfully |
FRET-ACCEPTOR | Synthesized Successfully |
ECOLI DESIGN PARTS
Part | Synthesis Status |
---|---|
FRET-DONOR | Synthesized Successfully |
FRET-ACCEPTOR | Synthesized Successfully |
HIGH POTENTIAL IRON SULFUR PROTEIN (HIPIP) | Synthesized Successfully |
PH RESISTANCE | Synthesized Successfully |
TETRATHIONATE HYDROLASE | Synthesized Successfully |
MRUBY 2 | Not Synthesized Successfully |
NowGFP | Not Synthesized Successfully |
PARTS DIGESTED AND TRANSFORMED
Complete Total Number of Transformations = 5
Successful number of Transformations = 2
Number of failed Transformations = 3
Results pH Resistance Gene
Total number of Transformations = 3
Successful number of Transformations = 2
Number of failed transformations = 1
Total number of gels runs = 3
Successful number of gel runs = 1
Number of failed gel runs = 2
From the gel image in figure 1 above it is evident that the pH resistance gene was inserted in the plasmid, however due to a small concentration of the DNA present, the band of the insert seemed to be blurred out.
From the results obtained and displayed in the graph above, it is evident that the pH resistance gene is effective for pH levels ranging from 5.5 to 7.5, where 7.5 is the pH level of the original media for the bacterial growth. It is also observed that, the resistance gene was not effective with pH levels 4.5 and below, resulting in no bacterial growth over time.