Difference between revisions of "Team:AshesiGhana/Results"

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<h2 class="border" style="margin-top: 5vh; text-align: center">Results</h2>
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<br/>
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<div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important">
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    <h4><font color="#111">Part Synthesis Results</font></h4>
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    <hr class="dark">
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<p><b>ACIDITHIOBACILLUS FEROXIDANS DESIGN PARTS</b></p>
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<table class="table table-bordered table-striped">
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<thead>
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        <tr>
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            <th>Part</th>
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            <th>Synthesis Status</th>
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        </tr>
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    </thead>
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    <tbody>
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        <tr>
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            <td>ORIGIN OF REPLICATION <img src="https://static.igem.org/mediawiki/2017/5/52/T--AshesiGhana--orep.png"></td>           
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            <td>Not Synthesized Successfully</td>
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        </tr>
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        <tr>
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            <td>FRET-DONOR <img src="https://static.igem.org/mediawiki/2017/6/6a/T--AshesiGhana--fretdon.png" ></td>
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            <td>Synthesized Successfully</td>
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        </tr>
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        <tr>
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            <td>FRET-ACCEPTOR<img src="https://static.igem.org/mediawiki/2017/9/9b/T--AshesiGhana--fretacc.png"></td>
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            <td>Synthesized Successfully</td>
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        </tr>
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    </tbody>
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</table>
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<p><b>ECOLI DESIGN PARTS</b></p>
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<table class="table table-bordered table-striped">
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<thead>
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        <tr>
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            <th>Part</th>
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            <th>Synthesis Status</th>
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        </tr>
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    </thead>
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    <tbody>
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        <tr>
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            <td>FRET-DONOR <img src="https://static.igem.org/mediawiki/2017/6/6a/T--AshesiGhana--fretdon.png" ></td>
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            <td>Synthesized Successfully</td>
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        </tr>
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        <tr>
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            <td>FRET-ACCEPTOR<img src="https://static.igem.org/mediawiki/2017/9/9b/T--AshesiGhana--fretacc.png"></td>
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            <td>Synthesized Successfully</td>
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        </tr>
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              <tr>
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            <td>HIGH POTENTIAL IRON SULFUR PROTEIN (HIPIP)<img src="https://static.igem.org/mediawiki/2017/7/75/T--AshesiGhana--hipip.png"></td>           
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            <td>Synthesized Successfully</td>
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        </tr>
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        <tr>
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            <td>PH RESISTANCE<img src="https://static.igem.org/mediawiki/2017/8/8e/T--AshesiGhana--hpresist.png"></td>
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            <td>Synthesized Successfully</td>
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        </tr>
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              <tr>
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            <td>TETRATHIONATE HYDROLASE<img src="https://static.igem.org/mediawiki/2017/3/36/T--AshesiGhana--tetrahydro.png"></td>           
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            <td>Synthesized Successfully</td>
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        </tr>
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            <tr>
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            <td>MRUBY 2<img src=""></td>           
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            <td>Not Synthesized Successfully</td>
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        </tr>
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            <tr>
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            <td>NowGFP<img src=""></td>           
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            <td>Not Synthesized Successfully</td>
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        </tr>
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    </tbody>
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</table>
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  </div>
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<div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important">
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  <center>
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    <h4><font color="#111">PARTS DIGESTED AND TRANSFORMED</font></h4>
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    <hr class="dark">
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  </center>
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<p><font color="#111"><br>
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<ol style="font-size:16px;">
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<li><b>Make up ligation reaction as below</b><br></li>
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<li><b>High Potential Iron Sulphur Protein (HiPiP)</b><br></li>
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<li><b>Tetrathionate  Hydrolase</b><br></li>
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<li><b>Fret-Donor </b><br></li>
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<li><b>Fret-Acceptor</b><br></li>
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</ol>
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<br>
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<br>
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<b>Complete Total Number of Transformations = 5</b><br>
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<b>Successful number of Transformations = 2</b><br>
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<b>Number of failed Transformations = 3</b><br>
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</font></p>
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  </div>
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<br>
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<br>
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<div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important">
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  <center>
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    <h4><font color="#111">Results pH Resistance Gene</font></h4>
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    <hr class="dark">
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  </center>
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<p><font color="#111"><b>Total number of Transformations = 3</b><br>
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<b>Successful number of Transformations = 2</b><br>
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<b>Number of failed transformations = 1</b><br>
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</font></p>
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<p><font color="#111"><b>Total number of gels runs = 3</b><br>
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<b>Successful number of gel runs = 1</b><br>
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<b>Number of failed gel runs = 2</b><br>
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</font></p>
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<br>
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<img src="https://static.igem.org/mediawiki/2017/f/f8/T--AshesiGhana--gelimage.jpg">
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<br>
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<p><font color="#111">From the gel image in figure 1 above it is evident that the pH resistance gene was inserted in the plasmid, however due to a small concentration of the DNA present, the band of the insert seemed to be blurred out.
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</font></p>
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<br>
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<img src="https://static.igem.org/mediawiki/2017/a/aa/T--AshesiGhana--effgraph.jpg">
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<br>
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<p><font color="#111">From the results obtained and displayed in the graph above, it is evident that the pH resistance gene is effective for pH levels ranging from 5.5 to 7.5, where 7.5 is the pH level of the original media for the bacterial growth. It is also observed that, the resistance gene was not effective with pH levels 4.5 and below, resulting in no bacterial growth over time.
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</font></p>
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<div class="clear" style="padding: 0px 12vw; border: 3px solid transparent; background-color: #fbf9f8!important">
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    <h4><font color="#111">Results FRET Donor</font></h4>
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    <hr class="dark">
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  </center>
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<p><font color="#111"><b>Total number of Transformations = 5</b><br>
 +
<b>Successful number of Transformations = 2</b><br>
 +
<b>Number of failed transformations = 3</b><br>
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</font></p>
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<p><font color="#111"><b>Total number of gels runs = 3</b><br>
 +
<b>Successful number of gel runs = 0</b><br>
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<b>Number of failed gel runs = 3</b><br>
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</font></p>
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<br>
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<img src="https://static.igem.org/mediawiki/2017/d/d9/T--AshesiGhana--fretdoneff.png">
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<br>
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<br>
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<br>
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<br>
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<img src="https://static.igem.org/mediawiki/2017/c/c2/T--AshesiGhana--abso.png">
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<br>
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<p><font color="#111">From figure 4 and 3 above, it is observed that the absorption and fluorescence in the sample increased over time as the bacteria growth increased, however the change was not significant and this concludes that the FRET-Donor is not as effective as expected. This could however be attributed to the fact that concentration of the Donor DNA isn’t enough to produce the expected high absorption and fluorescence values.
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{{Template:AshesiGhana/Footer}}

Latest revision as of 03:01, 2 November 2017

Ashesi University
College
HOME
TEAM
PROJECT
Overview
Description
Results
Demonstration
Medal Criteria
Achievement
NOTEBOOK
Protocols
Diary
Lab Book
InterLab
Measurement
PARTS
Parts
Basic Parts
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Improved Parts
Part Collection
HUMAN PRACTICES
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Water Industry
Integrated and Gold
Intellectual Property
Public Engagement
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MODELLING
Overview
D. O. Experiment
Continuous Culture
PHO Operon
JUDGING FORM

Results


Part Synthesis Results

ACIDITHIOBACILLUS FEROXIDANS DESIGN PARTS

Part Synthesis Status
ORIGIN OF REPLICATION Not Synthesized Successfully
FRET-DONOR Synthesized Successfully
FRET-ACCEPTOR Synthesized Successfully

ECOLI DESIGN PARTS

Part Synthesis Status
FRET-DONOR Synthesized Successfully
FRET-ACCEPTOR Synthesized Successfully
HIGH POTENTIAL IRON SULFUR PROTEIN (HIPIP) Synthesized Successfully
PH RESISTANCE Synthesized Successfully
TETRATHIONATE HYDROLASE Synthesized Successfully
MRUBY 2 Not Synthesized Successfully
NowGFP Not Synthesized Successfully

PARTS DIGESTED AND TRANSFORMED


  1. Make up ligation reaction as below
  2. High Potential Iron Sulphur Protein (HiPiP)
  3. Tetrathionate Hydrolase
  4. Fret-Donor
  5. Fret-Acceptor


Complete Total Number of Transformations = 5
Successful number of Transformations = 2
Number of failed Transformations = 3



Results pH Resistance Gene

Total number of Transformations = 3
Successful number of Transformations = 2
Number of failed transformations = 1

Total number of gels runs = 3
Successful number of gel runs = 1
Number of failed gel runs = 2



From the gel image in figure 1 above it is evident that the pH resistance gene was inserted in the plasmid, however due to a small concentration of the DNA present, the band of the insert seemed to be blurred out.



From the results obtained and displayed in the graph above, it is evident that the pH resistance gene is effective for pH levels ranging from 5.5 to 7.5, where 7.5 is the pH level of the original media for the bacterial growth. It is also observed that, the resistance gene was not effective with pH levels 4.5 and below, resulting in no bacterial growth over time.

Results FRET Donor

Total number of Transformations = 5
Successful number of Transformations = 2
Number of failed transformations = 3

Total number of gels runs = 3
Successful number of gel runs = 0
Number of failed gel runs = 3







From figure 4 and 3 above, it is observed that the absorption and fluorescence in the sample increased over time as the bacteria growth increased, however the change was not significant and this concludes that the FRET-Donor is not as effective as expected. This could however be attributed to the fact that concentration of the Donor DNA isn’t enough to produce the expected high absorption and fluorescence values.