Difference between revisions of "Team:CCU Taiwan/Device detection"

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<div id="Devices">
 
<div id="Devices">
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<h2>Device detection</h2>
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<h2>Detection</h2>
  
 
<p>
 
<p>
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For our detecting, we use Arduino UNO with a light to frequency converter to detect the light intensity of GFP, and a color detector to distinguish the color of the test paper.
+
We used Arduino UNO with a light frequency converter to detect the light intensity of GFP, and a color detector to distinguish the color of the test paper.
 
</p>
 
</p>
 
<br/>
 
<br/>
 
</div>
 
</div>
  
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<div id="light">
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<h3>Fluorescence detection</h3>
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<div class="aaa"></div>
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<p>
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<h3>Detecting of the light intensity</h3>
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First, we set the light frequency converter(TSL235R) to measure the light intensity of GFP emitted from our CSP and Lactic acid detectors.<br>
 +
Secondly, we created a standard curve by using a measuring instrument in our laboratory to detect the light intensity emitted from known concentrations of CSP and Lactic acid precisely.<br>
 +
Finally, we compared the light intensity detected by our device with the standard curve to determine the concentration of CSP or Lactic acid in the sample.<br>
  
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    <p>
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</p>
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      First, We select the light to frequency converter to receive the light intensity of GFP which is emitted from our CSP and Lactic acid,
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and we collect the frequency from the output of converter for every detection.
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Second, we use the measuring instrument in the laboratory to detect accurately to know the light intensity emitted from our CSP and Lactic acid.<br/>
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Finally, we model the curve for the light intensity and the frequency, so we can know the light intensity relatively when we collect the frequency from the converter.<br/>
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The following curve is the light intensity of GFP corresponded to the frequency of the converter.<br/><br/>
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<img src="https://static.igem.org/mediawiki/2017/d/d4/F_to_d.png" style="display:block; margin:auto;"><br/>
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    </p>
 
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</div>
 
  
−
 
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<h3>Optical system</h3>
 +
<p>
 +
For the detection of the fluorescence emitted by the bacteria after it combines with the targets, a monochromatic light(485nm) was set to excite the fluorescent protein. An optical sensor(TSL235R), two lens and a longpass filter(510nm) were placed to filter the unwanted light and focus the rest onto the optical sensor to detect its fluorescence intensity.
 +
</p>
  
 +
<img src="https://static.igem.org/mediawiki/2017/6/69/DR1.png" style="display:block; margin:auto;"><br/>
 +
 +
 +
<h3>The monochromatic light</h3>
 +
<p>
 +
The GFP we were using was GFPmut3. Picture A shows the excitation maxima of GFPmut3 is 501nm, and picture B shows the emission maxima is 511nm.
 +
</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2017/e/e7/DR2.png" style="display:block; margin:auto;"><br/>
  
  
  
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<div id="pH">
 
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<div class="aaa"></div>
 
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<h3>Detecting of the pH value</h3>
 
  
−
    <p>
 
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We choose the test paper changing its color from pH5.0 to pH8.0, for example, when the color of the test paper changing from red to green, the pH valut changing from 5 to 8,
 
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and then use the color detector to detect the test paper and collect the value of RGB, so we can know the pH value after we draw the curve between the value of RGB and the pH value.<br/>
 
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<br/>
 
−
<img src="https://static.igem.org/mediawiki/2017/c/ca/Ph_v.png" style="display:block; margin:auto;"><br/>
 
  
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Final, these five values (two for the light intensity, and three for the values of Red, Green, and Blue ) we receved will be transmitted from Arduino by our Bluetooth, HC-06, to the APP.
 
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    </p>
 
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<br/>
 
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</div>
 
  
  
  
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<div id="Materials">
 
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<div class="aaa"></div>
 
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<h3>Materials</h3>
 
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<p>
 
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Arduino Uno
 
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Test paper<br/>
 
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TSL 235R<br/>
 
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510nm Filter<br/>
 
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485nm LED<br/>
 
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TCS3200<br/>
 
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HC-06<br/>
 
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Convex lens<br/>
 
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Small Breadbroad<br/>
 
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Male to Female DuPont Breadboard Jumper Wires
 
  
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</p>
 
  
−
</div>
 
 
</section>
 
</section>
  

Revision as of 07:09, 1 November 2017

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Detection

We used Arduino UNO with a light frequency converter to detect the light intensity of GFP, and a color detector to distinguish the color of the test paper.


Fluorescence detection

First, we set the light frequency converter(TSL235R) to measure the light intensity of GFP emitted from our CSP and Lactic acid detectors.
Secondly, we created a standard curve by using a measuring instrument in our laboratory to detect the light intensity emitted from known concentrations of CSP and Lactic acid precisely.
Finally, we compared the light intensity detected by our device with the standard curve to determine the concentration of CSP or Lactic acid in the sample.

Optical system

For the detection of the fluorescence emitted by the bacteria after it combines with the targets, a monochromatic light(485nm) was set to excite the fluorescent protein. An optical sensor(TSL235R), two lens and a longpass filter(510nm) were placed to filter the unwanted light and focus the rest onto the optical sensor to detect its fluorescence intensity.


The monochromatic light

The GFP we were using was GFPmut3. Picture A shows the excitation maxima of GFPmut3 is 501nm, and picture B shows the emission maxima is 511nm.