Week 01 (2017/07/02~2017/07/08)
- Lactate detection system
Gene design of lactate detector
- CSP detection system
Gene design of CSP detector
Week 02 (2017/07/09~2017/07/15)
- Lactate detection system
Gene design of lactate detector
- CSP detection system
Gene design of CSP detector
Request 2 backbone plasmids of Bacillus subtilis from iGEM
| Part requesting #1 | BBa_K823022 | pSBBs4S |
|---|---|---|
| Part requesting #2 | BBa_K823023 | pSBBs1C |
Order chemical materials for preparing competent cell of Bacillus subtilis
Week 03 (2017/07/16~2017/07/22)
- Lactate detection system
Gene design of lactate detector
- CSP detection system
Gene design of CSP detector
Week 04 (2017/07/23~2017/07/29)
- Lactate detection system
Send orders to IDT for DNA synthesis
- CSP detection system
Gene design of CSP detector
Week 05 (2017/07/30~2017/08/05)
- Participate the 5th iGEM Asia-Pacific Conference at NCTU
Week 06 (2017/08/06~2017/08/12)
- Lactate detection system
- CSP detection system
Prepare competent cell of Bacillus subtilis
- Amplify two backbone plasmids of Bacillus subtilis
- InterLab Study
Transform eight kinds of plasmids into E. coli DH5α
| Positive Control | BBa_I20270 |
|---|---|
| Negative Control | BBa_R0040 |
| Test Device 1 | BBa_J364000 |
| Test Device 2 | BBa_J364001 |
| Test Device 3 | BBa_J364002 |
| Test Device 4 | BBa_J364003 |
| Test Device 5 | BBa_J364004 |
| Test Device 6 | BBa_J364005 |
Plate culture eight kinds of E. coli DH5α
Week 07 (2017/08/13~2017/08/19)
- Lactate detection system
Amplify the IDT plasmid of lactate detection system
| IDT plasmid # 1 | LldR gene on IDT vector |
|---|---|
| IDT plasmid # 2 | GFP gene on IDT vector |
| IDT plasmid # 3 | MazEF gene on IDT vector |
- CSP detection system
Amplify the IDT plasmid of CSP detection system
| IDT plasmid # 1 | ComE gene on IDT vector |
|---|
Week 08 (2017/08/20~2017/08/26)
- Lactate detection system
Restriction map analysis of IDT Plasmid
- CSP detection system
Restriction map analysis of B. subtilis backbone plasmid
Week 09 (2017/08/27~2017/09/02)
- Lactate detection system
Function test of kill switch (mazEF)
- CSP detection system
Transformation test of pSBBs1C and pSBBs4S
Week 10 (2017/09/03~2017/09/09)
- Lactate detection system
Amplify the backbone plasmid pSB1A2
- CSP detection system
Transformation test of pSBBs1C and pSBBs4S
Amplify the IDT plasmid of CSP detection system
| IDT plasmid # 2 | ComD gene on IDT plasmid |
|---|---|
| IDT plasmid # 3 | GFP gene on IDT plasmid |
- Test paper experiment
Small scale culture with test paper
Freeze dry the test paper
Week 11 (2017/09/10~2017/09/16)
- Lactate detection system
Restriction map analysis of pSB1A2
- CSP detection system
Restriction map analysis of IDT plasmid
- InterLab study
Construct OD600 reference point
Construct fluorescence standard curve
Week 12 (2017/09/17~2017/09/23)
- Lactate detection system
pSB1A2_GFP_LldR assembly
pSB1C3_mazEF assembly
- CSP detection system
pSBBS1C_comE assembly
- InterLab study
OD600 and fluorescence measurement of cell
Week 13 (2017/09/24~2017/09/30)
- Lactate detection system
Restriction map analysis of recombination DNA
- CSP detection system
pSBBs1C_comD assembly
- InterLab study
E-mail InterLab data to iGEM
Fill out three online form of InterLab
- Test paper experiment
Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper
Freeze dry the test paper
Week 14 (2017/10/01~2017/10/07)
- Lactate detection system
Function test of lactate detector
Function test of kill switch
- CSP detection system
pSBBs1C_comE_comD assembly
pSBBs4S_GFP assembly
- Test paper experiment
Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper
Freeze dry the liquid culture
Week 15 (2017/10/08~2017/10/14)
- Lactate detection system
Double plasmid transformation test
Function test of lactate detector
Function test of kill switch
- CSP detection system
Check the size of assembly plasmid
Transform the recombination plasmid to B. subtilis
- Test paper experiment
Revive the freeze-dried bacteria
Week 16 (2017/10/15~2017/10/21)
- Lactate detection system
Bacteria which contain two plasmid
- CSP detection system
Prepare competent cell of B. subtilis
Transform the recombination plasmid to B. subtilis
- Test paper experiment
Freeze dry the liquid culture, and then revive it
