Difference between revisions of "Team:Moscow RF/Demonstrate"

Line 1: Line 1:
−
{{Moscow_RF}}
+
{{Template:Moscow_RF}}
  
 
<html>
 
<html>
Line 7: Line 7:
 
<div class="column full_size">
 
<div class="column full_size">
 
<h1>Demonstrate</h1>
 
<h1>Demonstrate</h1>
−
<h3>Gold Medal Criterion #4</h3>
 
−
 
−
<p>
 
−
Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
 
−
</p>
 
−
 
−
<p>
 
−
Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
 
−
</p>
 
  
  
 +
<p><b>To test the hypothesis that our yeast ‘capsules’ and molecule modification can increase thermal stability of Obesumbacterium proteus phytase we will carry out the following experiment.</b></p>
 
</div>
 
</div>
  
 +
<div class="column triple_size" >
 +
<img src="https://static.igem.org/mediawiki/2017/c/cf/Demonstrate_RF.jpg"><br />
  
−
<div class="column half_size">
+
<p><b>1</b> – normal phytase, control (homogenate) </p>
 +
<p><b>2</b> – normal phytase, experiment (intact cells) </p>
 +
<p><b>3</b> – modified phytase, control (homogenate) </p>
 +
<p><b>4</b> – modified phytase, experiment (intact cells) </p>
  
−
<h4> What should we do for our demonstration?</h4>
+
<p>As shown in the picture, reaction has passed in both experiment wells and has not passed in the control wells. Besides, we can see that the radius of activity of the modified phytase exceeds the radius of activity of the normal phytase. This is due to the fact that when heated, the cell wall can reduce thermal impact destroying the enzyme but cannot fully protect it from such impact. Besides, normal phytase is partially destroyed because of proteolysis in yeasts and efficiency of the sample decreases, while the modified phytase retains its efficiency as in our project we have increased its stability by protecting its N-end with a compact protein</p>
  
−
<h5> Standard teams </h5>
 
−
 
−
<p>
 
−
If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
−
</p>
 
 
</div>
 
</div>
  
−
<div class="column half_size">
 
−
 
−
<br>
 
−
<h5> Special track teams </h5>
 
−
 
−
<p>
 
−
Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
 
−
</p>
 
−
 
−
 
−
</div>
 
  
  
  
 
</html>
 
</html>

Revision as of 10:19, 30 October 2017

HOME
TEAM
PROJECT DESIGN
Description
Notebook
Contribution
Model
Demonstrate
Attributions
PARTS
Parts
Basic Parts
Part Collection
SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
JUDGING FORM
THANKS

Demonstrate

To test the hypothesis that our yeast ‘capsules’ and molecule modification can increase thermal stability of Obesumbacterium proteus phytase we will carry out the following experiment.


1 – normal phytase, control (homogenate)

2 – normal phytase, experiment (intact cells)

3 – modified phytase, control (homogenate)

4 – modified phytase, experiment (intact cells)

As shown in the picture, reaction has passed in both experiment wells and has not passed in the control wells. Besides, we can see that the radius of activity of the modified phytase exceeds the radius of activity of the normal phytase. This is due to the fact that when heated, the cell wall can reduce thermal impact destroying the enzyme but cannot fully protect it from such impact. Besides, normal phytase is partially destroyed because of proteolysis in yeasts and efficiency of the sample decreases, while the modified phytase retains its efficiency as in our project we have increased its stability by protecting its N-end with a compact protein