|
|
Line 260: |
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| </table> | | </table> |
| <p> Order chemical materials for preparing competent cell of <i>Bacillus subtilis</i></p> | | <p> Order chemical materials for preparing competent cell of <i>Bacillus subtilis</i></p> |
| + | |
| + | <h3>Week 03 (2017/07/16~2017/07/22)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Gene design of lactate detector</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Gene design of CSP detector</p> |
| + | |
| + | <h3>Week 04 (2017/07/23~2017/07/29)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Send orders to IDT for DNA synthesis</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Gene design of CSP detector</p> |
| + | |
| + | <h3>Week 05 (2017/07/30~2017/08/05)</h3> |
| + | <ol><li>Participate the 5th iGEM Asia-Pacific Conference at NCTU</li></ol> |
| + | |
| + | <h3>Week 06 (2017/08/06~2017/08/12)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Prepare competent cell of <i>Bacillus subtilis</i><br/> |
| + | - Amplify two backbone plasmids of <i>Bacillus subtilis</i> |
| + | </p> |
| + | <ol><li>InterLab Study</li></ol> |
| + | <p> Transform eight kinds of plasmids into <i>E. coli DH5α</i></p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg .tg-yw4l{vertical-align:top} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">Positive Control</th> |
| + | <th class="tg-031e">BBa_I20270</th> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">Negative Control</td> |
| + | <td class="tg-031e">BBa_R0040</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 1</td> |
| + | <td class="tg-yw4l">BBa_J364000</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 2</td> |
| + | <td class="tg-yw4l">BBa_J364001</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 3</td> |
| + | <td class="tg-yw4l">BBa_J364002</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 4</td> |
| + | <td class="tg-yw4l">BBa_J364003</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 5</td> |
| + | <td class="tg-yw4l">BBa_J364004</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 6</td> |
| + | <td class="tg-yw4l">BBa_J364005</td> |
| + | </tr> |
| + | </table> |
| + | <p> Plate culture eight kinds of <i>E. coli DH5α</i></p> |
| + | |
| + | <h3>Week 07 (2017/08/13~2017/08/19)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Amplify the IDT plasmid of lactate detection system</p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">IDT plasmid # 1</th> |
| + | <th class="tg-031e">LldR gene on IDT vector</th> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">IDT plasmid # 2</td> |
| + | <td class="tg-031e">GFP gene on IDT vector</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">IDT plasmid # 3</td> |
| + | <td class="tg-031e">MazEF gene on IDT vector</td> |
| + | </tr> |
| + | </table> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Amplify the IDT plasmid of CSP detection system</p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">IDT plasmid # 1</th> |
| + | <th class="tg-031e">ComE gene on IDT vector</th> |
| + | </tr> |
| + | </table> |
| + | |
| + | <h3>Week 08 (2017/08/20~2017/08/26)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Restriction map analysis of IDT Plasmid</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Restriction map analysis of <i>B. subtilis</i> backbone plasmid</p> |
| + | |
| + | <h3>Week 09 (2017/08/27~2017/09/02)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Function test of kill switch (mazEF)</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Transformation test of pSBBs1C and pSBBs4S</p> |
| + | |
| + | <h3>Week 10 (2017/09/03~2017/09/09)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Amplify the backbone plasmid pSB1A2</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Transformation test of pSBBs1C and pSBBs4S<br/> |
| + | Amplify the IDT plasmid of CSP detection system |
| + | </p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">IDT plasmid # 2</th> |
| + | <th class="tg-031e">ComD gene on IDT plasmid</th> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">IDT plasmid # 3</td> |
| + | <td class="tg-031e">GFP gene on IDT plasmid</td> |
| + | </tr> |
| + | </table> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> |
| + | Small scale culture with test paper<br/> |
| + | Freeze dry the test paper |
| + | </p> |
| + | |
| + | <h3>Week 11 (2017/09/10~2017/09/16)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Restriction map analysis of pSB1A2</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Restriction map analysis of IDT plasmid</p> |
| + | <ol><li>InterLab study</li></ol> |
| + | <p> |
| + | Construct OD600 reference point<br/> |
| + | Construct fluorescence standard curve |
| + | </p> |
| + | |
| + | <h3>Week 12 (2017/09/17~2017/09/23)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | pSB1A2_GFP_LldR assembly<br/> |
| + | pSB1C3_mazEF assembly |
| + | </p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> pSBBS1C_comE assembly</p> |
| + | <ol><li>InterLab study</li></ol> |
| + | <p> OD600 and fluorescence measurement of cell</p> |
| + | |
| + | <h3>Week 13 (2017/09/24~2017/09/30)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | Restriction map analysis of recombination DNA</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> pSBBs1C_comD assembly</p> |
| + | <ol><li>InterLab study</li></ol> |
| + | <p> |
| + | E-mail InterLab data to iGEM<br/> |
| + | Fill out three online form of InterLab |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> |
| + | Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper<br/> |
| + | Freeze dry the test paper |
| + | </p> |
| + | |
| + | <h3>Week 14 (2017/10/01~2017/10/07)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | Function test of lactate detector<br/> |
| + | Function test of kill switch |
| + | </p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | pSBBs1C_comE_comD assembly<br/> |
| + | pSBBs4S_GFP assembly |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> |
| + | Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper<br/> |
| + | Freeze dry the liquid culture |
| + | </p> |
| + | |
| + | <h3>Week 15 (2017/10/08~2017/10/14)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | Double plasmid transformation test<br/> |
| + | Function test of lactate detector<br/> |
| + | Function test of kill switch |
| + | </p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Check the size of assembly plasmid<br/> |
| + | Transform the recombination plasmid to <i>B. subtilis</i> |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> Revive the freeze-dried bacteria</p> |
| + | |
| + | |
| + | <h3>Week 16 (2017/10/15~2017/10/21)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Bacteria which contain two plasmid</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Prepare competent cell of <i>B. subtilis</i><br/> |
| + | Transform the recombination plasmid to <i>B. subtilis</i> |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> Freeze dry the liquid culture, and then revive it</p> |
| | | |
| </section> | | </section> |