Team:Moscow RF/Demonstrate
Demonstrate
To test the hypothesis that our yeast ‘capsules’ and molecule modification can increase thermal stability of Obesumbacterium proteus phytase we will carry out the following experiment.
1 – normal phytase, control (homogenate)
2 – normal phytase, experiment (intact cells)
3 – modified phytase, control (homogenate)
4 – modified phytase, experiment (intact cells)
As shown in the picture, reaction has passed in both experiment wells and has not passed in the control wells. Besides, we can see that the radius of activity of the modified phytase exceeds the radius of activity of the normal phytase. This is due to the fact that when heated, the cell wall can reduce thermal impact destroying the enzyme but cannot fully protect it from such impact. Besides, normal phytase is partially destroyed because of proteolysis in yeasts and efficiency of the sample decreases, while the modified phytase retains its efficiency as in our project we have increased its stability by protecting its N-end with a compact protein
