Team:CCU Taiwan/Notebook

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Week 01 (2017/07/02~2017/07/08)

  1. Lactate detection system

          Gene design of lactate detector

  1. CSP detection system

          Gene design of CSP detector

Week 02 (2017/07/09~2017/07/15)

  1. Lactate detection system

          Gene design of lactate detector

  1. CSP detection system

          Gene design of CSP detector
          Request 2 backbone plasmids of Bacillus subtilis from iGEM

Part requesting #1 BBa_K823022 pSBBs4S
Part requesting #2 BBa_K823023 pSBBs1C

          Order chemical materials for preparing competent cell of Bacillus subtilis

Week 03 (2017/07/16~2017/07/22)

  1. Lactate detection system

          Gene design of lactate detector

  1. CSP detection system

          Gene design of CSP detector

Week 04 (2017/07/23~2017/07/29)

  1. Lactate detection system

          Send orders to IDT for DNA synthesis

  1. CSP detection system

          Gene design of CSP detector

Week 05 (2017/07/30~2017/08/05)

  1. Participate the 5th iGEM Asia-Pacific Conference at NCTU

Week 06 (2017/08/06~2017/08/12)

  1. Lactate detection system
  1. CSP detection system

          Prepare competent cell of Bacillus subtilis
          - Amplify two backbone plasmids of Bacillus subtilis

  1. InterLab Study

          Transform eight kinds of plasmids into E. coli DH5α

Positive Control BBa_I20270
Negative Control BBa_R0040
Test Device 1 BBa_J364000
Test Device 2 BBa_J364001
Test Device 3 BBa_J364002
Test Device 4 BBa_J364003
Test Device 5 BBa_J364004
Test Device 6 BBa_J364005

          Plate culture eight kinds of E. coli DH5α

Week 07 (2017/08/13~2017/08/19)

  1. Lactate detection system

          Amplify the IDT plasmid of lactate detection system

IDT plasmid # 1 LldR gene on IDT vector
IDT plasmid # 2 GFP gene on IDT vector
IDT plasmid # 3 MazEF gene on IDT vector
  1. CSP detection system

          Amplify the IDT plasmid of CSP detection system

IDT plasmid # 1 ComE gene on IDT vector

Week 08 (2017/08/20~2017/08/26)

  1. Lactate detection system

          Restriction map analysis of IDT Plasmid

  1. CSP detection system

          Restriction map analysis of B. subtilis backbone plasmid

Week 09 (2017/08/27~2017/09/02)

  1. Lactate detection system

          Function test of kill switch (mazEF)

  1. CSP detection system

          Transformation test of pSBBs1C and pSBBs4S

Week 10 (2017/09/03~2017/09/09)

  1. Lactate detection system

          Amplify the backbone plasmid pSB1A2

  1. CSP detection system

          Transformation test of pSBBs1C and pSBBs4S
          Amplify the IDT plasmid of CSP detection system

IDT plasmid # 2 ComD gene on IDT plasmid
IDT plasmid # 3 GFP gene on IDT plasmid
  1. Test paper experiment

          Small scale culture with test paper
          Freeze dry the test paper

Week 11 (2017/09/10~2017/09/16)

  1. Lactate detection system

          Restriction map analysis of pSB1A2

  1. CSP detection system

          Restriction map analysis of IDT plasmid

  1. InterLab study

          Construct OD600 reference point
          Construct fluorescence standard curve

Week 12 (2017/09/17~2017/09/23)

  1. Lactate detection system

          pSB1A2_GFP_LldR assembly
          pSB1C3_mazEF assembly

  1. CSP detection system

          pSBBS1C_comE assembly

  1. InterLab study

          OD600 and fluorescence measurement of cell

Week 13 (2017/09/24~2017/09/30)

  1. Lactate detection system

          Restriction map analysis of recombination DNA

  1. CSP detection system

          pSBBs1C_comD assembly

  1. InterLab study

          E-mail InterLab data to iGEM
          Fill out three online form of InterLab

  1. Test paper experiment

          Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper
          Freeze dry the test paper

Week 14 (2017/10/01~2017/10/07)

  1. Lactate detection system

          Function test of lactate detector
          Function test of kill switch

  1. CSP detection system

          pSBBs1C_comE_comD assembly
          pSBBs4S_GFP assembly

  1. Test paper experiment

          Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper
          Freeze dry the liquid culture

Week 15 (2017/10/08~2017/10/14)

  1. Lactate detection system

          Double plasmid transformation test
          Function test of lactate detector
          Function test of kill switch

  1. CSP detection system

          Check the size of assembly plasmid
          Transform the recombination plasmid to B. subtilis

  1. Test paper experiment

          Revive the freeze-dried bacteria

Week 16 (2017/10/15~2017/10/21)

  1. Lactate detection system

          Bacteria which contain two plasmid

  1. CSP detection system

          Prepare competent cell of B. subtilis
          Transform the recombination plasmid to B. subtilis

  1. Test paper experiment

          Freeze dry the liquid culture, and then revive it

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