Difference between revisions of "Team:CCU Taiwan/Attributions"

 
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<a href="#Fluorescein">Fluorescein curve</a>
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<a href="#att">Attribution</a>
 
<ul>
 
<ul>
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<li><a href="#Fluorescein-Plate-reader">Plate reader</a></li>
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<li><a href="#t-acc">Team Accomplished</a></li>
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<li><a href="#Fluorescein-Material">Material</a></li>
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<li><a href="#fun">Fundraising help and advice</a></li>
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<li><a href="#Fluorescein-Method">Method</a></li>
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<li><a href="#lab">Lab support</a></li>
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<li><a href="#Fluorescein-Data-result">Data result</a></li>
+
<li><a href="#dts">Difficult technique support</a></li>
 +
                                <li><a href="#pas">Project advisor support</a></li>
 +
                                <li><a href="#pc">Presentation coaching</a></li>
 +
                                <li><a href="#hps">Human Practices support</a></li>
 +
                                <li><a href="#ttps">Team Training and Project Start</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
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<li>
 
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<a href="#OD600">OD600 Reference point</a>
 
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      <ul>
 
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<li><a href="#OD600-Plate-reader">Plate reader</a></li>
 
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<li><a href="#OD600-Material">Material</a></li>
 
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<li><a href="#OD600-Method">Method</a></li>
 
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<li><a href="#OD600-Data-result">Data result</a></li>
 
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      </ul>
 
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</li>
 
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<li>
 
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<a href="#Cell">Cell measure</a>
 
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      <ul>
 
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<li><a href="#Cell-Material">Material</a></li>
 
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<li><a href="#Cell-Method">Method</a></li>
 
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<li><a href="#Cell-Data-result">Data result</a></li>
 
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   <section>
 
   <section>
  
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<div id="att">
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<div id="Fluorescein">
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<h2>Attribution</h2>
−
<h2>Fluorescein Fluorescence standard curve</h2>
+
<p>
−
 
+
Without the help of so many people so far, we won’t become who we are now.<br/>
 +
Without these people supporting our dream, CCU_Taiwan would never have formed.<br/>
 +
We are very grateful for your help, for what you did for us during this year. We believed your support will bring us success!
 +
</p>
 
</div>
 
</div>
  
−
<div id="Fluorescein-Plate-reader">
+
<div id="t-acc">
 
<div class="aaa"></div>
 
<div class="aaa"></div>
−
<h3>Plate reader</h3>
+
<h3>【Team Accomplished】</h3>
 +
<h4>Primary PI:</h4>
 +
<ol><li>Cheng-I Lee: Main instructor, plan implement support, provide technical resources and fundraising advices, administrative mediator.</li></ol><br/>
  
−
    <p>
+
<h4>Instructor:</h4>
−
      microplate reader FLUOstar Omega</br>
+
<ol><li>Hau-Ren Chen: Instruction on experiment techniques, materials resources support, team operation system advisor.</li></ol>
−
emission filter: 520 nm</br>
+
<ol><li>Victoria Rau: Instructor on English training, including presentation, poster, wiki.</li></ol>
−
excitation filter: 485 nm
+
<ol><li>Gerry Rau: Instructor on English training, including presentation, poster, wiki.</li></ol>
−
    </p>
+
<ol><li>Sung-Nien Yu: Give advices about instrument aspect, especially on photometric system.</li></ol>
 +
<ol><li>Wen-Hsin Hsieh: Instruction on device structure, especially on composites.</li></ol>
 +
<ol><li>Guo-En Chang: Give advices on the design of the glass slides.</li></ol>
 +
<ol><li>Chen-Kuo Chiang: Machine learning instructor.</li></ol>
 +
<ol><li>Ren-Hung Hwang: Internet of Thing, IoT system instructor.</li></ol>
 +
<br/>
  
−
</div>
+
<h4>Advisor:</h4>
−
 
+
<ol><li>Cheng-Ping Jheng, a PhD student in Molecular biology. He gave us technique support, recommendation on data analyzing and experiment designing, and had a "HAPPY" summer with us.
−
<div id="Fluorescein-Material">
+
.</li></ol><br/>
−
<div class="aaa"></div>
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−
<h3>Material</h3>
+
−
<p>
+
−
Fluorescein sodium salt</br>
+
−
1xPBS</br>
+
−
Tissue culture testplate (black with flat bottom)
+
−
</p>
+
  
−
</div>
+
<h4>Team Leader:</h4>
 +
<ol><li>Shin-Yung Chen: Project manager, trouble shooting, deal with funds, get the team together, contact teachers, wet lab support (part of some experiments and problem shooting), and presenter.</li></ol>
 +
<ol><li>Yu-Hsuan Huang: Project manager, trouble shooting, deal with funds, get the team together, contact teachers, dry lab support (part of IoT system,web site coding and machine learning), and presenter.</li></ol><br/>
 +
 
 +
<h4>Wet Lab:</h4>
 +
<ol><li>Fang-Yi Lo: Group leader, keep wet lab on schedule. Choose the best kind of test paper, leting bacterial be attached strongly to it. Wiki article writer.</li></ol>
 +
<ol><li>Chung-Hao Lee: Group leader, keep wet lab on schedule. Lactate detector gene designer, lactate kill switch experiment, and wiki article writer.</li></ol>
 +
<ol><li>Zi-You Lin: Experiment material controller, CSP detector experiment, modeling, and wiki article writer.</li></ol>
 +
<ol><li>Fei Chen: Lactate kill switch system designer, lactate detector experiment, modeling,  equipment manager, and presenter.</li></ol>
 +
<ol><li>Shun-Tian Tchen: CSP detector system gene designer, test paper experiment, English trainer, presenter and art designer (including our uniform, banner, poster, name card…and so on).</li></ol>
 +
<ol><li>Yi-Chia Su: CSP detector experiment, modeling, cooperate with CSMU_NCHU_Taiwan, chemical material manager, presenter.</li></ol>
 +
<ol><li>Cheng-Yu Lee: Lactate detector experiment, the interlab, poster.</li></ol>
 +
<ol><li>Yi-Chen Hsieh: Cooperate with NYMU_Taipei, poster.</li></ol><br/>
 +
 
 +
 
 +
<h4>Dry Lab:</h4>
 +
<ol><li>Chih-Yu Lin: Group leader, keep dry lab on schedule. Coding for App, IoT system between App and server, wiki page website, machine learning, and poster.</li></ol>
 +
<ol><li>Lai-Sum Yim: Device designer, including the shelter and the inner design. Wiki article writer.</li></ol>
 +
<ol><li>Chia-Hui Liu: Light intensity sensor designer, Arduino coding, IoT system between carindex and cellphone in bluetooth, control motors, and wiki article writer.</li></ol>
 +
<ol><li>Weng-San Lei: Device, consumptive material modifier, uniform order, and wiki article writer.</li></ol><br/>
  
−
<div id="Fluorescein-Method">
 
−
<div class="aaa"></div>
 
−
                <h3>Method</h3>
 
−
 
−
<ol><li>Prepare fluorescein stock solution</li></ol>
 
−
<p>
 
−
1. Spin down fluorescein stock tube to make sure pellet is at the bottom of tube.</br>
 
−
2. Prepare 2x fluorescein stock solution (100 μM) by resuspending fluorescein in 1 mL of 1xPBS. </br>
 
−
3. Dilute the 2x fluorescein stock solution with 1xPBS to make a 1x fluorescein solution and resulting concentration of fluorescein  stock solution 50 μM </br></br>
 
−
</p>
 
−
<ol><li>Serial dilutions</li></ol>
 
−
<p>
 
−
1. Add 100 μl of PBS into wells A2, B2, C2, D2....A12, B12, C12, D12</br>
 
−
2. Add 200 μl of fluorescein 1x stock solution into A1, B1, C1, D1</br>
 
−
3. Transfer 100 μl of fluorescein stock solution from A1 into A2. </br>
 
−
4. Mix A2 by pipetting up and down 3x and transfer 100 μl into A3. </br>
 
−
5. Mix A3 by pipetting up and down 3x and transfer 100 μl into A4. </br>
 
−
6. Mix A4 by pipetting up and down 3x and transfer 100 μl into A5. </br>
 
−
7. Mix A5 by pipetting up and down 3x and transfer 100 μl into A6. </br>
 
−
8. Mix A6 by pipetting up and down 3x and transfer 100 μl into A7. </br>
 
−
9. Mix A7 by pipetting up and down 3x and transfer 100 μl into A8. </br>
 
−
10. Mix A8 by pipetting up and down 3x and transfer 100 μl into A9. </br>
 
−
11. Mix A9 by pipetting up and down 3x and transfer 100 μl into A10.</br>
 
−
12. Mix A10 by pipetting up and down 3x and transfer 100 μl into A11. </br>
 
−
13. Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.
 
−
  (Caution: Do not to continue serial dilution into column 12.)</br>
 
−
</p>
 
−
<ol><li>repeat serial dilute for Row B、D、E</strong></li></ol>
 
−
<ol><li>Measure fluorescence of all samples in all standard measurement modes in instrument Record the data in your notebook</strong></li></ol>
 
−
<ol><li>Import data into Excel (fluorescein standard curve tab ) Sheet_1 provided</li></ol>
 
−
 
−
<br/><br/>
 
  
 +
<h4>Human Practice:</h4>
 +
<ol><li>Shing-Feng Sue: Group leader, keep human practice on schedule. Market survey, gene survey, and wiki article writer.</li></ol>
 +
<ol><li>Wan-I Lin: Market survey , gene survey, business plan, and presenter.</li></ol><br/>
 
</div>
 
</div>
  
−
<div id="Fluorescein-Data-result">
+
<div id="fun">
 
<div class="aaa"></div>
 
<div class="aaa"></div>
−
<h3>Data result</h3>
+
<h3>【Fundraising help and advice】</h3>
−
<br/>
+
<ol><li>We received helps from National Chung Cheng University Administrative Members including principal, vice-principal, deans and vice-deans of college of science, engineering, management and media faculty, etc. With their helps, we successfully hold our fundraising event and earned our fees.</li></ol>
−
<img src="https://static.igem.org/mediawiki/2017/8/85/FFs_1.png" style="display:block; margin:auto;"><br/><br/>
+
<ol><li>With the helps from Department of Biomedical Sciences, Physics, International Affair and Education Development Center, etc, we got sufficient fund to purchase our instruments and chemicals.</li></ol>
−
<img src="https://static.igem.org/mediawiki/2017/a/a4/FFs_2.png" style="display:block; margin:auto;"><br/><br/>
+
<ol><li>Our fund is sponsored by the enthusiastic alumni and entrepreneurs.</li></ol><br/>
−
<img src="https://static.igem.org/mediawiki/2017/5/5c/FFs_3.png" style="display:block; margin:auto;"><br/>
+
 
</div>
 
</div>
−
</section>
 
−
 
−
<section>
 
−
 
−
 
−
<div id="OD600">
 
−
<h2>OD600 Reference point</h2>
 
  
 +
<div id="lab">
 +
<div class="aaa"></div>
 +
<h3>【Lab support】</h3>
 +
<ol><li>Public instruments were provided by National Chung Cheng University Department of Bio-medics.</li></ol>
 +
<ol><li>National Chung Cheng University Department of Biomedical Sciences teacher Yu-Wei Leu providing us Nanodrop so that we can measure DNA concentration and quality.</li></ol>
 +
<ol><li>National Chung Cheng University Department of Biomedical Sciences teacher Hsien-Bin Huang lend us freeze-dryer so that we can make our test paper.</li></ol>
 +
<ol><li>National Chung Cheng University Department of Chemistry and Biochemistry teacher Biing-Chau Tzheng and teacher Ching-Ching Yu provide us NH<sub>4</sub>CL so that we can make M9 medium.</li></ol>
 +
<ol><li>National Chung Cheng University Department of Chemistry and Biochemistry teacher Jong-Yuh Cherng provide starch soluble to let us make starch plate.</li></ol>
 +
<ol><li>National Chung Cheng University College of Science principal Lai-Kwan Chau provide us laser cutter to let us create our device composite.</li></ol>
 +
<ol><li>National Chung Cheng University Department of Mechanical Engineering De-Shin Liu support us in 3D printing during device crafting.</li></ol>
 +
<ol><li>Rong-Bin Li, a student in MS program in Molecular biology. He helped us on trouble shooting, gave us recommendation on data analyzing and experiment designing, provided experimental instrument, and had a "HAPPY" summer with us.</li></ol>
 
</div>
 
</div>
  
−
 
+
<div id="dts">
−
<div id="OD600-Plate-reader">
+
 
<div class="aaa"></div>
 
<div class="aaa"></div>
−
<h3>Plate reader</h3>
+
<h3>【Difficult technique support】</h3>
−
 
+
<ol><li>Buddhist Tzu-chi General Hospital Division of Clinical Pathology to offer us lactate concentration measuring instruments and experiments.</li></ol>
−
    <p>
+
<ol><li>Dentists from Bai-Li dental office, Qiu dental office, Li-Xiang dental office help us define our oral condition, provide us the raw data for machine learning.</li></ol><br/>
−
Thermo Scientific™ Multiskan™ FC Filter-based Microplate Photometer</br>
+
−
Filter: 595 nm</br>
+
−
    </p>
+
−
 
+
 
</div>
 
</div>
  
−
<div id="OD600-Material">
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<div id="pas">
−
  <div class="aaa"></div>
+
−
<h3>Material</h3>
+
−
<p>
+
−
1 ml LUDOX</br>
+
−
mQH<sub>2</sub>O</br>
+
−
96 well cell culture plate (clear with flat-bottom)
+
−
</p>
+
−
 
+
−
</div>
+
−
 
+
−
<div id="OD600-Method">
+
 
<div class="aaa"></div>
 
<div class="aaa"></div>
−
                <h3>Method</h3>
+
<h3>【Project advisor support】</h3>
−
 
+
<ol><li>National Yang-Ming University teacher Chuan-Hsiung Chang, National Chiao-Tung University teacher Wen-Liang Chen helped us found our team and understand iGEM. Also, they gave us many precious advices during brain storming.</li></ol>
−
<p>
+
<ol><li>Missouri University of Science and Technology iGEM Team advisor Dr. David J. Westenberg gave us many advice and ideas regarding the training and brain storming at the beginning of the iGEM competition.</li></ol>
−
1. Add 100 μl LUDOX into wells A1, B1, C1, D1 (or 1 mL LUDOX into cuvette)</br>
+
<ol><li>National Taiwan University of Science and Technology teacher Hsiu-Mei Chen showed us the technology of bacteriorhodopsin chip. This let us burst more ideas about our project.</li></ol>
−
2. Add 100 μl of H<sub>2</sub>O into wells A2, B2, C2, D2 (or 1 mL H<sub>2</sub>O into cuvette)</br>
+
<ol><li>Renew Biocare Corporation CEO Ully & Victoria provided us ideas and critiques from professional perspectives.</li></ol>
−
3. Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</br>
+
<ol><li>YunTech Cheng-Guang Chen gave us advice on product development</li></ol><br/>
−
4. Record the data in excel and Import data into Excel ( OD600 reference point tab ) Sheet_1 provided</br>
+
−
</p>
+
−
+
 
</div>
 
</div>
  
−
<div id="OD600-Data-result">
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<div id="pc">
−
<div class="aaa"></div>
+
<div class="aaa"></div>
−
<h3>Data result</h3>
+
<h3>【Presentation coaching】</h3>
−
<br/>
+
<ol><li>Taishin Financial Holding Co. Director of Human Resource Affair Janice instructed us presentation, powerpoint making.</li></ol>
−
<img src="https://static.igem.org/mediawiki/2017/c/cd/Ee.jpeg" style="display:block; margin:auto;"><br/>
+
<ol><li>National Chung Cheng University Department of Chemistry and Biochemistry teacher Yuan-Yao Lip offer us a big occasion to practice our presentation.</li></ol><br/>
 
</div>
 
</div>
−
</section>
 
−
 
−
<section>
 
−
 
−
 
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<div id="Cell">
 
−
<h2>Cell measure</h2>
 
  
 +
<div id="hps">
 +
<div class="aaa"></div>
 +
<h3>【Human Practices support】</h3>
 +
<ol><li>National Chung Cheng University Department of Financial & Economic Law teacher Wen-Yin Chen gave us instruction regarding patent.</li></ol>
 +
<ol><li>National Chung Cheng University Department of Business Administration teacher Shih-Chieh Chuang instructed us about business model.</li></ol>
 +
<ol><li>YunTech Department of Accounting teacher Cheng-Guang Chen gave us advices on team management.</li></ol>
 +
<ol><li>Local industry Kumkwat Farm help us set up a booth activity to communicate with the public and sponsor the lottery prizes.</li></ol><br/>
 
</div>
 
</div>
  
−
 
+
<div id="ttps">
−
<div id="Cell-Material">
+
−
  <div class="aaa"></div>
+
−
<h3>Material</h3>
+
−
<p>
+
−
Competent cells ( Escherichia coli strain DH5α)</br>
+
−
LB (Luria Bertani) media</br>
+
−
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 μg /mL)</br>
+
−
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)</br>
+
−
Incubator at 37°C</br>
+
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1.5 ml eppendorf tubes for sample storage</br>
+
−
Ice bucket with ice</br>
+
−
Pipettes</br>
+
−
96 well plate(cell culture 96 well plate、tissue culture testplate)</br>
+
−
Devices (from InterLab Measurement Kit):</br>
+
−
1. Negative control(BBa_R0040)</br>
+
−
2. Positive control(J23151+B0032+E0040+B0010+B0012)</br>
+
−
3. Test Device 1: J23101+I13504</br>
+
−
4. Test Device 2: J23106+I13504</br>
+
−
5. Test Device 3: J23117+I13504</br>
+
−
6. Test Device 4: J23101+BCD2+E0040+B0015</br>
+
−
7. Test Device 5: J23106+BCD2+E0040+B0015</br>
+
−
8. Test Device 6: J23117+BCD2+E0040+B0015</br>
+
−
</p>
+
−
 
+
−
</div>
+
−
 
+
−
<div id="Cell-Method">
+
 
<div class="aaa"></div>
 
<div class="aaa"></div>
−
                <h3>Method</h3>
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<h3>【Team Training and Project Start】</h3>
−
 
+
 
<p>
 
<p>
−
1. Day 1 : Resuspended each plasmid in plate 7 and transform into Escherichia coli DH5α.</br>
+
Q：Does your institution teach an iGEM or synthetic biology course? When did you start this course?<br/>
−
&nbsp;(Transformation protocol is from iGEM)</br>
+
A：As CCU_Taiwan formed the very first iGEM Team of National Chung Cheng University, things start to move, to change, gradually. Because of the formation of CCU_Taiwan, our school designed a whole new interdisciplinary course in 2017 summer. What’s more, two interdisciplinary projects were launched in the 2017 new school semester!<br/>
−
2. Day 2 : Pick 2 colonies from each of plate and inoculate it on 5-10 mL LB medium +Chloramphenicol.Grow the cells overnight (16-18 hours) at 37°C and 170 rpm.</br>
+
<br/>
−
3. Day 3 : Set instrument to read OD600 (as OD calibration setting)and measure OD600 of the overnight cultures</br>
+
Q：When did you start your brain storming?<br/>
−
4. Dilute the cultures to a target OD 600 of 0.02 in 12 ml LB medium + Chloramphenicol in 50 mL falcon tube (covered with foil to block light).</br>
+
A：We started our brain storming from Oct of 2016, and we filter our ideas through the below thinking model:
−
5. Incubate the cultures at 37°C and 170 rpm.</br>
+
What problem do we encounter by our side? -> Is this problem already solved? -> If not, it is because no one has done it or it is too difficult to solve? -> Can we solve this problem? -> Project determine!<br/>
−
6. Take 1000 μL samples of the cultures at 0, 2, 4, and 6 hours of incubation and place samples on ice</br>
+
<br/>
−
7. 4 replicates of 100 uL samples were taken from each culture at 0, 2, 4, and 6 hours of incubation and placed in a 96 well plate for OD and fluorescence measurements using the setup described above</br>
+
Q：When did you start in the lab?<br/>
 +
A：Our experiment training took place in Feb of 2017. And the actual project lab work started from Aug of 2017.<br/>
 +
<br/>
 +
Q：When did you start working on your project?<br/>
 +
A：Right after the project was determined in April, we started seeking the method to finish it. During this period, we managed to learn a lot of thing that is required (such as joining Taiwan MIT mini bootcamp to know more about entrepreneurship). We finished our gene design at July, and decided what software we had to build. We started rushing for our project in summer.<br/>
 
</p>
 
</p>
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</div>
 
</div>
  
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<div id="Cell-Data-result">
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<div class="aaa"></div>
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<h3>Data result</h3>
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<br/>
+
−
<img src="https://static.igem.org/mediawiki/2017/f/f7/IL_cell1.jpg" style="display:block; margin:auto;"><br/><br/>
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<img src="https://static.igem.org/mediawiki/2017/4/41/IL_cell2.jpg" style="display:block; margin:auto;"><br/>
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</div>
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</section>
 
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              <a href="emailto:ccu.igem.2017@gmail.com">
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              <img src="https://static.igem.org/mediawiki/2016/e/e2/T--Harvard_BioDesign--images_gmail01.png" alt="Email Logo" style="width:51px;height:51px;">
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<!-- Copyright -->
 
<!-- Copyright -->
 
<div class="copyright">
 
<div class="copyright">
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<ul class="menu">
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<li>&#169; 2017 CCU Taiwan iGEM</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
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Latest revision as of 13:48, 14 December 2017

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Attribution

Without the help of so many people so far, we won’t become who we are now.
Without these people supporting our dream, CCU_Taiwan would never have formed.
We are very grateful for your help, for what you did for us during this year. We believed your support will bring us success!

【Team Accomplished】

Primary PI:

  1. Cheng-I Lee: Main instructor, plan implement support, provide technical resources and fundraising advices, administrative mediator.

Instructor:

  1. Hau-Ren Chen: Instruction on experiment techniques, materials resources support, team operation system advisor.
  1. Victoria Rau: Instructor on English training, including presentation, poster, wiki.
  1. Gerry Rau: Instructor on English training, including presentation, poster, wiki.
  1. Sung-Nien Yu: Give advices about instrument aspect, especially on photometric system.
  1. Wen-Hsin Hsieh: Instruction on device structure, especially on composites.
  1. Guo-En Chang: Give advices on the design of the glass slides.
  1. Chen-Kuo Chiang: Machine learning instructor.
  1. Ren-Hung Hwang: Internet of Thing, IoT system instructor.

Advisor:

  1. Cheng-Ping Jheng, a PhD student in Molecular biology. He gave us technique support, recommendation on data analyzing and experiment designing, and had a "HAPPY" summer with us. .

Team Leader:

  1. Shin-Yung Chen: Project manager, trouble shooting, deal with funds, get the team together, contact teachers, wet lab support (part of some experiments and problem shooting), and presenter.
  1. Yu-Hsuan Huang: Project manager, trouble shooting, deal with funds, get the team together, contact teachers, dry lab support (part of IoT system,web site coding and machine learning), and presenter.

Wet Lab:

  1. Fang-Yi Lo: Group leader, keep wet lab on schedule. Choose the best kind of test paper, leting bacterial be attached strongly to it. Wiki article writer.
  1. Chung-Hao Lee: Group leader, keep wet lab on schedule. Lactate detector gene designer, lactate kill switch experiment, and wiki article writer.
  1. Zi-You Lin: Experiment material controller, CSP detector experiment, modeling, and wiki article writer.
  1. Fei Chen: Lactate kill switch system designer, lactate detector experiment, modeling, equipment manager, and presenter.
  1. Shun-Tian Tchen: CSP detector system gene designer, test paper experiment, English trainer, presenter and art designer (including our uniform, banner, poster, name card…and so on).
  1. Yi-Chia Su: CSP detector experiment, modeling, cooperate with CSMU_NCHU_Taiwan, chemical material manager, presenter.
  1. Cheng-Yu Lee: Lactate detector experiment, the interlab, poster.
  1. Yi-Chen Hsieh: Cooperate with NYMU_Taipei, poster.

Dry Lab:

  1. Chih-Yu Lin: Group leader, keep dry lab on schedule. Coding for App, IoT system between App and server, wiki page website, machine learning, and poster.
  1. Lai-Sum Yim: Device designer, including the shelter and the inner design. Wiki article writer.
  1. Chia-Hui Liu: Light intensity sensor designer, Arduino coding, IoT system between carindex and cellphone in bluetooth, control motors, and wiki article writer.
  1. Weng-San Lei: Device, consumptive material modifier, uniform order, and wiki article writer.

Human Practice:

  1. Shing-Feng Sue: Group leader, keep human practice on schedule. Market survey, gene survey, and wiki article writer.
  1. Wan-I Lin: Market survey , gene survey, business plan, and presenter.

【Fundraising help and advice】

  1. We received helps from National Chung Cheng University Administrative Members including principal, vice-principal, deans and vice-deans of college of science, engineering, management and media faculty, etc. With their helps, we successfully hold our fundraising event and earned our fees.
  1. With the helps from Department of Biomedical Sciences, Physics, International Affair and Education Development Center, etc, we got sufficient fund to purchase our instruments and chemicals.
  1. Our fund is sponsored by the enthusiastic alumni and entrepreneurs.

【Lab support】

  1. Public instruments were provided by National Chung Cheng University Department of Bio-medics.
  1. National Chung Cheng University Department of Biomedical Sciences teacher Yu-Wei Leu providing us Nanodrop so that we can measure DNA concentration and quality.
  1. National Chung Cheng University Department of Biomedical Sciences teacher Hsien-Bin Huang lend us freeze-dryer so that we can make our test paper.
  1. National Chung Cheng University Department of Chemistry and Biochemistry teacher Biing-Chau Tzheng and teacher Ching-Ching Yu provide us NH4CL so that we can make M9 medium.
  1. National Chung Cheng University Department of Chemistry and Biochemistry teacher Jong-Yuh Cherng provide starch soluble to let us make starch plate.
  1. National Chung Cheng University College of Science principal Lai-Kwan Chau provide us laser cutter to let us create our device composite.
  1. National Chung Cheng University Department of Mechanical Engineering De-Shin Liu support us in 3D printing during device crafting.
  1. Rong-Bin Li, a student in MS program in Molecular biology. He helped us on trouble shooting, gave us recommendation on data analyzing and experiment designing, provided experimental instrument, and had a "HAPPY" summer with us.

【Difficult technique support】

  1. Buddhist Tzu-chi General Hospital Division of Clinical Pathology to offer us lactate concentration measuring instruments and experiments.
  1. Dentists from Bai-Li dental office, Qiu dental office, Li-Xiang dental office help us define our oral condition, provide us the raw data for machine learning.

【Project advisor support】

  1. National Yang-Ming University teacher Chuan-Hsiung Chang, National Chiao-Tung University teacher Wen-Liang Chen helped us found our team and understand iGEM. Also, they gave us many precious advices during brain storming.
  1. Missouri University of Science and Technology iGEM Team advisor Dr. David J. Westenberg gave us many advice and ideas regarding the training and brain storming at the beginning of the iGEM competition.
  1. National Taiwan University of Science and Technology teacher Hsiu-Mei Chen showed us the technology of bacteriorhodopsin chip. This let us burst more ideas about our project.
  1. Renew Biocare Corporation CEO Ully & Victoria provided us ideas and critiques from professional perspectives.
  1. YunTech Cheng-Guang Chen gave us advice on product development

【Presentation coaching】

  1. Taishin Financial Holding Co. Director of Human Resource Affair Janice instructed us presentation, powerpoint making.
  1. National Chung Cheng University Department of Chemistry and Biochemistry teacher Yuan-Yao Lip offer us a big occasion to practice our presentation.

【Human Practices support】

  1. National Chung Cheng University Department of Financial & Economic Law teacher Wen-Yin Chen gave us instruction regarding patent.
  1. National Chung Cheng University Department of Business Administration teacher Shih-Chieh Chuang instructed us about business model.
  1. YunTech Department of Accounting teacher Cheng-Guang Chen gave us advices on team management.
  1. Local industry Kumkwat Farm help us set up a booth activity to communicate with the public and sponsor the lottery prizes.

【Team Training and Project Start】

Q：Does your institution teach an iGEM or synthetic biology course? When did you start this course?
A：As CCU_Taiwan formed the very first iGEM Team of National Chung Cheng University, things start to move, to change, gradually. Because of the formation of CCU_Taiwan, our school designed a whole new interdisciplinary course in 2017 summer. What’s more, two interdisciplinary projects were launched in the 2017 new school semester!

Q：When did you start your brain storming?
A：We started our brain storming from Oct of 2016, and we filter our ideas through the below thinking model: What problem do we encounter by our side? -> Is this problem already solved? -> If not, it is because no one has done it or it is too difficult to solve? -> Can we solve this problem? -> Project determine!

Q：When did you start in the lab?
A：Our experiment training took place in Feb of 2017. And the actual project lab work started from Aug of 2017.

Q：When did you start working on your project?
A：Right after the project was determined in April, we started seeking the method to finish it. During this period, we managed to learn a lot of thing that is required (such as joining Taiwan MIT mini bootcamp to know more about entrepreneurship). We finished our gene design at July, and decided what software we had to build. We started rushing for our project in summer.