Difference between revisions of "Team:US AFRL CarrollHS/Experiments"

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     <h1>Experiment</h1>
 
     <h1>Experiment</h1>
 
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    </div>
 
   </div>
 
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<li>Thaw 50 µL vial of BL21 cells on ice</li>
 
<li>Thaw 50 µL vial of BL21 cells on ice</li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells<li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells<li>
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<li.a>incubate on ice for 30 minutes</li>
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<l>incubate on ice for 30 minutes</li>
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<li.a>heat shock at 42℃ for 10 seconds</li>
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<li.b>heat shock at 42℃ for 10 seconds</li>
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<li.a>incubate on ice for 5m</li>
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<li.b>incubate on ice for 5m</li>
 
<li>Inoculate 250µL LB into vial</li>
 
<li>Inoculate 250µL LB into vial</li>
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<li.a>incubate at 37℃ with shaking for 1.5 hours</li>
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<li.b>incubate at 37℃ with shaking for 1.5 hours</li>
 
<li>Plate 1x/9x</li>
 
<li>Plate 1x/9x</li>
 
</ol>
 
</ol>

Revision as of 21:54, 24 October 2017


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
  3. incubate on ice for 30 minutes
    4. heat shock at 42℃ for 10 seconds incubate on ice for 5m
  4. Inoculate 250µL LB into vial
    5. incubate at 37℃ with shaking for 1.5 hours
  5. Plate 1x/9x

Methodology