|
|
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| tender supervision of knowledgable mentors, the students learned to accurately | | tender supervision of knowledgable mentors, the students learned to accurately |
| master lab techniques that would prove invaluable once lab work began.</p> | | master lab techniques that would prove invaluable once lab work began.</p> |
| + | |
| </div> | | </div> |
| | | |
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| probability that the E. coli absorbed the plasmid. The tired but excited young | | probability that the E. coli absorbed the plasmid. The tired but excited young |
| researchers went home with fingers crossed.</p> | | researchers went home with fingers crossed.</p> |
| + | <p><b>Packaging: </b>The lab members attended a review meeting for the Materials Lab. This is where people gave mini presentations on how their projects were going and what problems they have run into. The Packaging members and Chia considered the advantages of using different surface proteins in order to package E. coli and decided on curli. The four Packaging students searched the iGEM site for parts (cellulose binding domains, Csg A, etc) was discussed. |
| + | </p> |
| | | |
| <h3>Thursday (06/08/17)</h3> | | <h3>Thursday (06/08/17)</h3> |
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| to confirm that E.coli JM109 had absorbed the | | to confirm that E.coli JM109 had absorbed the |
| plasmid PRhl_GFPa1</p> | | plasmid PRhl_GFPa1</p> |
| + | <p><b>Packaging: </b>The teams attended a seminar given by a professor from Northwestern. The Packaging team clarified the goals of their project by deciding to attach a cellulose binding domain to the Csg A portion of a curli protein on E. coli bacteria. In the lab, the team worked with producing agarose gel, diluting a buffer, and using these components to run gel electrophoresis on DNA samples. Additionally, the team poured LB agar plates. |
| + | |
| + | </p> |
| | | |
| <h3>Friday (06/09/17)</h3> | | <h3>Friday (06/09/17)</h3> |
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| to measure how pure the DNA extracted is. For future experiments, the plasmid DNA | | to measure how pure the DNA extracted is. For future experiments, the plasmid DNA |
| would then be sent to an offsite lab to be sequenced.</p> | | would then be sent to an offsite lab to be sequenced.</p> |
| + | <p><b>Packaging: </b>The Packaging team prepared DNA samples and analyzed the samples with the Nanodrop device. Later, the team used restriction enzymes to slice open the DNA and then ran the samples through gel electrophoresis. The project idea continued to progress with the team finding parts form the iGEM inventory and researching DNA sequences of the various parts. |
| + | |
| + | </p> |
| + | |
| + | |
| <br> | | <br> |
| </div> | | </div> |
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| broth and agar. Later the team observed the purification of the PCR results and the | | broth and agar. Later the team observed the purification of the PCR results and the |
| analyzation of the results with Qubit.</p> | | analyzation of the results with Qubit.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (06/13/17)</h3> | | <h3>Tuesday (06/13/17)</h3> |
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| will turn green with the addition of the quorum sensing molecule to the bacteria.</p> | | will turn green with the addition of the quorum sensing molecule to the bacteria.</p> |
| <p>The next morning, the plasmid should glow green indicating it is present.</p> | | <p>The next morning, the plasmid should glow green indicating it is present.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Wednesday (06/14/17)</h3> | | <h3>Wednesday (06/14/17)</h3> |
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| experience the second time doing the mini-prep, the trailblazing students had a | | experience the second time doing the mini-prep, the trailblazing students had a |
| much better grasp and spent less time with more accuracy.</p> | | much better grasp and spent less time with more accuracy.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (06/15/17)</h3> | | <h3>Thursday (06/15/17)</h3> |
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| Things do not always work out in the lab | | Things do not always work out in the lab |
| and the students were eager to restart and achieve favorable results.</p> | | and the students were eager to restart and achieve favorable results.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (06/16/17)</h3> | | <h3>Friday (06/16/17)</h3> |
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| bonded over frozen custard and ice cream.</p> | | bonded over frozen custard and ice cream.</p> |
| </div> | | </div> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <div class="text2"> | | <div class="text2"> |
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| out a sigh of relief once the gel was safely placed in the tube and put in the fridge to | | out a sigh of relief once the gel was safely placed in the tube and put in the fridge to |
| be melted down tomorrow.</p> | | be melted down tomorrow.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (06/20/17)</h3> | | <h3>Tuesday (06/20/17)</h3> |
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| error and submitted defeat. The day was wrapped up by working hard designing the | | error and submitted defeat. The day was wrapped up by working hard designing the |
| wiki and learning how to code.</p> | | wiki and learning how to code.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Wednesday (06/21/17)</h3> | | <h3>Wednesday (06/21/17)</h3> |
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| Purification, which separated the massive amounts of DNA. The DNA was then | | Purification, which separated the massive amounts of DNA. The DNA was then |
| measured using the nano drop and these results were given:</p> | | measured using the nano drop and these results were given:</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (6/22/17)</h3> | | <h3>Thursday (6/22/17)</h3> |
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| to meet with the other lab students to discuss current progress. The students created | | to meet with the other lab students to discuss current progress. The students created |
| discussion points and had a great discussion with the other lab.</p> | | discussion points and had a great discussion with the other lab.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (6/23/17)</h3> | | <h3>Friday (6/23/17)</h3> |
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| leaders who wanted to know information about progress and future proceedings.</p> | | leaders who wanted to know information about progress and future proceedings.</p> |
| <br> | | <br> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| </div> | | </div> |
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| very uneven graph, but with the desired numerals. Afterwards, the mentors discussed | | very uneven graph, but with the desired numerals. Afterwards, the mentors discussed |
| and determined to still do a legation the next day and see the results.</p> | | and determined to still do a legation the next day and see the results.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (6/27/17)</h3> | | <h3>Tuesday (6/27/17)</h3> |
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| Afterwards, the groups planned out agenda and discussed necessary steps to | | Afterwards, the groups planned out agenda and discussed necessary steps to |
| complete the wiki page.</p> | | complete the wiki page.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Wednesday (6/28/17)</h3> | | <h3>Wednesday (6/28/17)</h3> |
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| before sending in the DNA for sequencing. Everyone shared "elbow-fives" and were | | before sending in the DNA for sequencing. Everyone shared "elbow-fives" and were |
| relieved that all their hard work had not gone to waste!</p> | | relieved that all their hard work had not gone to waste!</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (6/29/17)</h3> | | <h3>Thursday (6/29/17)</h3> |
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| of DNA. Everyone cheered as they realized that the "response" component of the | | of DNA. Everyone cheered as they realized that the "response" component of the |
| project was successful.</p> | | project was successful.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (6/30/17)</h3> | | <h3>Friday (6/30/17)</h3> |
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| improvements and wiki renovations. New logos were also created as well as | | improvements and wiki renovations. New logos were also created as well as |
| graphics. The students will continue to add content to the wiki weekly.</p> | | graphics. The students will continue to add content to the wiki weekly.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| + | |
| </div> | | </div> |
| | | |
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| presentation tips from mentors for the upcoming lab presentations the following | | presentation tips from mentors for the upcoming lab presentations the following |
| week.</p> | | week.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (7/6/16)</h3> | | <h3>Thursday (7/6/16)</h3> |
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| After reading through the protocols and laying out a plan of attack, the | | After reading through the protocols and laying out a plan of attack, the |
| students had the wait since the lab was going under routine inspection. The students | | students had the wait since the lab was going under routine inspection. The students |
− | spent the rest of the day designing more graphics and made wiki edits. In the | + | spent the rest of the day designing more graphics and made wiki edits. <br> |
| + | <p><b>Packaging: </b></p> |
| + | <p>In the |
| afternoon, the whole team got together with mentors to meet at the Air Force | | afternoon, the whole team got together with mentors to meet at the Air Force |
| Museum in Dayton, Ohio in order to take self pictures and group pictures for the | | Museum in Dayton, Ohio in order to take self pictures and group pictures for the |
− | wiki.</p><br> | + | wiki.</p> |
| + | |
| | | |
| </div> | | </div> |
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| postpone the InterLab studies and finished the day by creating a lot more plates | | postpone the InterLab studies and finished the day by creating a lot more plates |
| for future experiments.</p> | | for future experiments.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (7/11/17)</h3> | | <h3>Tuesday (7/11/17)</h3> |
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| the necessary forms for the InterLab as well as planned out the dropdown menu and the | | the necessary forms for the InterLab as well as planned out the dropdown menu and the |
| slideshow for the website.</p> | | slideshow for the website.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Wednesday (7/12/17)</h3> | | <h3>Wednesday (7/12/17)</h3> |
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| The students spent the rest of the day redoing the protocol in hopes of achieving the needed | | The students spent the rest of the day redoing the protocol in hopes of achieving the needed |
| amount of colonies in order to proceed.</p> | | amount of colonies in order to proceed.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (7/13/17)</h3> | | <h3>Thursday (7/13/17)</h3> |
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| the wiki. Tomorrow they will have s video conference with the U.S. Navy and U.S. Army iGem | | the wiki. Tomorrow they will have s video conference with the U.S. Navy and U.S. Army iGem |
| team.</p> | | team.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (7/14/17)</h3> | | <h3>Friday (7/14/17)</h3> |
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| Later in the afternoon, the students did a dry run of their presentation in preparation for | | Later in the afternoon, the students did a dry run of their presentation in preparation for |
| Monday.</p> | | Monday.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| + | |
| </div> | | </div> |
| | | |
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| up to date so the young researchers dedicated the rest of | | up to date so the young researchers dedicated the rest of |
| the day for wiki organization and miscellaneous tasks.</p> | | the day for wiki organization and miscellaneous tasks.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (7/18/17)</h3> | | <h3>Tuesday (7/18/17)</h3> |
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| described. Afterwards the students continued working on | | described. Afterwards the students continued working on |
| collaboration and organizing wiki pages.</p> | | collaboration and organizing wiki pages.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (7/20/17)</h3> | | <h3>Thursday (7/20/17)</h3> |
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| component and hope to achieve the end goal | | component and hope to achieve the end goal |
| soon.</p> | | soon.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (7/21/17)</h3> | | <h3>Friday (7/21/17)</h3> |
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| their weekly lab meeting with the RX lab and | | their weekly lab meeting with the RX lab and |
| shared the exciting news of their new discovery.</p><br> | | shared the exciting news of their new discovery.</p><br> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| </div> | | </div> |
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| to grow overnight in LB-Chl. They then left for the afternoon to get on wi-fi and worked on the | | to grow overnight in LB-Chl. They then left for the afternoon to get on wi-fi and worked on the |
| wiki and human outreach goals.</p> | | wiki and human outreach goals.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (7/25/17)</h3> | | <h3>Tuesday (7/25/17)</h3> |
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| disappointed to find out that their cultures had not grown and would need to | | disappointed to find out that their cultures had not grown and would need to |
| restart and pick other colonies.</p> | | restart and pick other colonies.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (7/27/17)</h3> | | <h3>Thursday (7/27/17)</h3> |
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| and proceed again tomorrow. Later in the day, the young researchers finalized their plan for | | and proceed again tomorrow. Later in the day, the young researchers finalized their plan for |
| "flat Stanley" and the outreach survey they hope to do in the future.</p> | | "flat Stanley" and the outreach survey they hope to do in the future.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (7/28/17)</h3> | | <h3>Friday (7/28/17)</h3> |
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| protocol with ease and completed the protocol | | protocol with ease and completed the protocol |
| accurately and quickly.</p> | | accurately and quickly.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| + | |
| </div> | | </div> |
| | | |
Line 510: |
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| cut enzymes PacI and BmrI. The Digest was then confirmed using a gel, which | | cut enzymes PacI and BmrI. The Digest was then confirmed using a gel, which |
| showed the accurate number of base pairs.</p> | | showed the accurate number of base pairs.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (8/1/17)</h3> | | <h3>Tuesday (8/1/17)</h3> |
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| Kinetics reading of the Plate Reader. The kinetics were taken over 24 hours, every | | Kinetics reading of the Plate Reader. The kinetics were taken over 24 hours, every |
| 20 minutes. The students then worked on the wiki, trying to create a template.</p> | | 20 minutes. The students then worked on the wiki, trying to create a template.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Wednesday (8/2/17)</h3> | | <h3>Wednesday (8/2/17)</h3> |
Line 533: |
Line 643: |
| | | |
| performed another digest using the PCR they had just purified.</p> | | performed another digest using the PCR they had just purified.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (8/4/17)</h3> | | <h3>Friday (8/4/17)</h3> |
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| to perform the transformation on the plasmid. The students followed the protocol exactly | | to perform the transformation on the plasmid. The students followed the protocol exactly |
| and were able to achieve favorable results.</p><br> | | and were able to achieve favorable results.</p><br> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| </div> | | </div> |
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| Therefore Mentor Mike recommended that they re-do the ligation from Monday. The ligation | | Therefore Mentor Mike recommended that they re-do the ligation from Monday. The ligation |
| went very smoothly and the transformation will be done tomorrow.</p> | | went very smoothly and the transformation will be done tomorrow.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Tuesday (8/8/17)</h3> | | <h3>Tuesday (8/8/17)</h3> |
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| completion of their "sense" component. The students left the lab in high spirits and | | completion of their "sense" component. The students left the lab in high spirits and |
| headed out for wifi and collaboration space to discuss more wiki work and future work.</p> | | headed out for wifi and collaboration space to discuss more wiki work and future work.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Wednesday (8/9/17)</h3> | | <h3>Wednesday (8/9/17)</h3> |
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| the colonies were already glowing green. With already putting in a long day of work the | | the colonies were already glowing green. With already putting in a long day of work the |
| students made overnights for the next day.</p> | | students made overnights for the next day.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Thursday (8/10/17)</h3> | | <h3>Thursday (8/10/17)</h3> |
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| presentations. This was especially helpful for the students, and gave them many | | presentations. This was especially helpful for the students, and gave them many |
| ideas on how to set up their poster.</p> | | ideas on how to set up their poster.</p> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| <h3>Friday (8/11/17)</h3> | | <h3>Friday (8/11/17)</h3> |
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| their good byes to their coworkers, whom they had enjoyed many lunch breaks with.</p> | | their good byes to their coworkers, whom they had enjoyed many lunch breaks with.</p> |
| </div> | | </div> |
| + | <p><b>Packaging: </b> |
| + | </p> |
| + | |
| | | |
| | | |