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| <h2>csgA-dCBD (Curli fibers attachable to cellulose)</h2> | | <h2>csgA-dCBD (Curli fibers attachable to cellulose)</h2> |
− | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522000">BBa_K2522000</a></h3> | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522000">csgA-dCBD (Curli fibers attachable to cellulose) BBa_K2522000</a></h3> |
| + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522003">Super Folder Green Fluorescent Proteien BBa_K2522003</a></h3> |
| <p>BBa_K2522000 is a plasmid containing a genetic sequence that expresses CsgA and dCBD continuously. The final product was developed in several steps. A part from the iGEM Distribution Kit, BBa_K1321340, was used. This part is the genetic code for a double cellulose binding domain, which functions to attach an organism to cellulose. The second piece of genetic code used was the <i>csgA</i> gene. </p> | | <p>BBa_K2522000 is a plasmid containing a genetic sequence that expresses CsgA and dCBD continuously. The final product was developed in several steps. A part from the iGEM Distribution Kit, BBa_K1321340, was used. This part is the genetic code for a double cellulose binding domain, which functions to attach an organism to cellulose. The second piece of genetic code used was the <i>csgA</i> gene. </p> |
| <p><i>csg</i> (curli specific gene) followed by a letter of the alphabet refers to the genes involved in expressing curli fibers. Curli fibers are hair-like strands of proteins generated by certain strains of <i>E. coli</i> on the outer membrane of the bacteria.. These proteins encompass the bacteria in a fibrous mesh. <i>csgA</i> in particular indicates the individual building blocks of the fibers.</p> | | <p><i>csg</i> (curli specific gene) followed by a letter of the alphabet refers to the genes involved in expressing curli fibers. Curli fibers are hair-like strands of proteins generated by certain strains of <i>E. coli</i> on the outer membrane of the bacteria.. These proteins encompass the bacteria in a fibrous mesh. <i>csgA</i> in particular indicates the individual building blocks of the fibers.</p> |
| <p>To construct the part, the genetic code for <i>csgA</i> was removed from the genomic DNA of <i>E. coli</i> Nissle and the dCBD was taken from the Distribution Kit. The genes were combined in a plasmid with the stop codon removed from the code for <i>csgA</i>. Using this process, the csgA flows directly into the dCBD, so that every time a CsgA unit is produced, a dCBD unit is attached to the <i>CsgA</i>. Because of this, the mesh of curli fibers that encompasses the microbe can bind to cellulose so that the microbe is encapsulated in cellulose. | | <p>To construct the part, the genetic code for <i>csgA</i> was removed from the genomic DNA of <i>E. coli</i> Nissle and the dCBD was taken from the Distribution Kit. The genes were combined in a plasmid with the stop codon removed from the code for <i>csgA</i>. Using this process, the csgA flows directly into the dCBD, so that every time a CsgA unit is produced, a dCBD unit is attached to the <i>CsgA</i>. Because of this, the mesh of curli fibers that encompasses the microbe can bind to cellulose so that the microbe is encapsulated in cellulose. |
| </p> | | </p> |
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− | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522000">csgA-dCBD (Curli fibers attachable to cellulose) BBa_K2522000</a></h3>
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− | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522003">Super Folder Green Fluorescent Protein BBa_K2522003</a></h3>
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