Biosensor
Lactate detection system
One of our biosensor is to detect the level of lactate. In order to achieve this purpose, we constructed a lactate detection system (BBa_K2292005) in E. coli.
In order to test our lactate detection system work or not, we add different concentrations of lactic acid into the liquid culture of E. coli DH5α which contain lactate detection system.
a. The fluorescence of each lactate concentration depends on time.
b. The fluorescence of different time point depends on lactate level.
c. Three-dimension diagram of fluorescence, time and lactate concentration
The fluorescence difference between each concentration of lactate is more obvious after 90 minutes. The experiment result demonstrates that the function of lactate detection is work.
CSP detection system
- PCR result：Prove that we successfully transformed our plasmid into E.coli, DH5α
i. pSBBS1C
ii. pSBBS4S
- Functional test result
According to the measurement of absorbance of wavelength 595 nm, we can confirm that CSP isn’t toxin to Bacillus Subtilis.
And for the measurement of the fluorescent plate reader (excitation: 485 nm, emission: 520 nm), we found out that the best amount of bacteria for measuring, the CSP conc. is at the value of OD600 = 0.2 after a series of experiment.
The intensity of fluorescence slowly drops after a sharp rise in a small amount of time, hence we can tell that the reaction starts right away as soon as CSP is added.
