Difference between revisions of "Team:CCU Taiwan/Device detection"

 
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
  
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Medals">Medals</a></li>
 
 
 
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<ul>
 
<ul>
 
<li>
 
<li>
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<a href="#Devices">Device detection</a>
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<a href="#Detection">Detection</a>
 
<ul>
 
<ul>
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<li><a href="#light">Light intensity</a></li>
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<li><a href="#Fluorescence">Fluorescence detection</a></li>
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<li><a href="#pH">pH value</a></li>
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<li><a href="#Optical">Optical system</a></li>
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<li><a href="#Materials">Materials</a></li>
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<li><a href="#The">Monochromatic light</a></li>
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<li><a href="#Experiment">Experiment</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
  
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<li>
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<a href="#pH">pH level</a>
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</li>
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<li>
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<a href="#Transmission">Transmission</a>
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</li>
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<li>
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<a href="#Future">Future Plan</a>
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</li>
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<li>
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<a href="#Materials">Materials</a>
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</li>
  
 
</ul>
 
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<div id="Devices">
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<div id="Detection">
 
<h2>Detection</h2>
 
<h2>Detection</h2>
  
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</div>
 
</div>
  
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<div id="Fluorescence">
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<div class="aaa"></div>
 
<h3>Fluorescence detection</h3>
 
<h3>Fluorescence detection</h3>
 
<p>
 
<p>
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</p>
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</div>
  
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<div id="Optical">
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<div class="aaa"></div>
 
<h3>Optical system</h3>
 
<h3>Optical system</h3>
 
<p>
 
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<img src="https://static.igem.org/mediawiki/2017/6/69/DR1.png" style="display:block; margin:auto;"><br/>
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<img src="https://static.igem.org/mediawiki/2017/4/4b/FFF.jpg" style="display:block; margin:auto;"><br/>
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</div>
  
  
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<div id="The">
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<div class="aaa"></div>
 
<h3>The monochromatic light</h3>
 
<h3>The monochromatic light</h3>
 
<p>
 
<p>
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<img src="https://static.igem.org/mediawiki/2017/e/e7/DR2.png" style="display:block; margin:auto;"><br/>
 
<img src="https://static.igem.org/mediawiki/2017/e/e7/DR2.png" style="display:block; margin:auto;"><br/>
  
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<p>
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The purple line is the 510nm filter what we use, but it’s starting point is not 510nm exactly. So we chose the 485nm light to avoid unwanted light detection.
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</p>
  
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<img src="https://static.igem.org/mediawiki/2017/0/0c/DR4.png" style="display:block; margin:auto;"><br/>
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</div>
  
  
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<div id="Experiment">
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<div class="aaa"></div>
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<h3>Experiment</h3>
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<p>
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The following curve is the fluorescein concentration corresponded to the frequency of the converter, so we know the lowest limit of our converter for detecting the fluorescein concentration is 6.25uM.
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</p>
  
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<img src="https://static.igem.org/mediawiki/2017/2/21/DR6.png" style="display:block; margin:auto;"><br/>
  
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<p>
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The next curve is the fluorescein concentration corresponded to the fluorescence in order to check whether two curves have similar positive correlation.
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</p>
  
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<img src="https://static.igem.org/mediawiki/2017/a/a7/DR5.png" style="display:block; margin:auto;"><br/>
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</div>
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<div id="pH">
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<h2>pH level</h2>
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<p>
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We chose a test paper with a range from pH 5.0 to pH 8.0. For example, when the color of the test paper changes from red to green, the pH value changes from 5 to 8. Color detection is used to to detect the test paper and register the RGB value.
 +
The RGB value allows us to measure the pH value.
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</p>
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<img src="https://static.igem.org/mediawiki/2017/c/ca/Ph_v.png" style="display:block; margin:auto;"><br/>
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</div>
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<div id="Transmission">
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<h2>Transmission</h2>
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<p>
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Five values (two for the fluorescence intensity, three for the RGB values) we received will be transmitted from Arduino to the APP by our Bluetooth, HC-06.
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</p>
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</div>
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<div id="Future">
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<h2>
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Future Plan
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</h2>
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<p>
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For the fluorescence intensity, we hope we can detect the larger range of the fluorescence intensity by the circuit we designed so that the accuracy can be improved.
 +
<br/>
 +
For the pH level, we hope we can plot the suitable curve or update our App to know the precise pH value in the future.
 +
 +
</p>
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</div>
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<div id="Materials">
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<h2>Materials</h2>
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<p>
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<li> Arduino Uno<br/>
 +
<li> Slides<br/>
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<li> Test paper<br/>
 +
<li> 485nm monochromatic light<br/>
 +
<li> 510nm filter <br/>
 +
<li> Lens<br/>
 +
<li> TSL 235R<br/>
 +
<li> TCS3200<br/>
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<li> HC-06<br/>
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<li> Small Breadbroad<br/>
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<li> Male to Female DuPont Breadboard Jumper Wires
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</p>
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</div>
  
  
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<!-- Copyright -->
 
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<div class="copyright">
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<ul class="menu">
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<li>&#169; 2017 CCU Taiwan iGEM</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
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<img src="https://static.igem.org/mediawiki/2017/4/48/%E7%BF%94%E5%AE%8F-logo.jpg" width="400" height="120">
 
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Latest revision as of 06:00, 15 December 2017

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Detection

We used Arduino UNO with a light frequency converter to detect the light intensity of GFP, and a color detector to distinguish the color of the test paper.


Fluorescence detection

First, we set the light frequency converter(TSL235R) to measure the light intensity of GFP emitted from our CSP and Lactic acid detectors.
Secondly, we created a standard curve by using a measuring instrument in our laboratory to detect the light intensity emitted from known concentrations of CSP and Lactic acid precisely.
Finally, we compared the light intensity detected by our device with the standard curve to determine the concentration of CSP or Lactic acid in the sample.

Optical system

For the detection of the fluorescence emitted by the bacteria after it combines with the targets, a monochromatic light(485nm) was set to excite the fluorescent protein. An optical sensor(TSL235R), two lens and a longpass filter(510nm) were placed to filter the unwanted light and focus the rest onto the optical sensor to detect its fluorescence intensity.


The monochromatic light

The GFP we were using was GFPmut3. Picture A shows the excitation maxima of GFPmut3 is 501nm, and picture B shows the emission maxima is 511nm.


The purple line is the 510nm filter what we use, but it’s starting point is not 510nm exactly. So we chose the 485nm light to avoid unwanted light detection.


Experiment

The following curve is the fluorescein concentration corresponded to the frequency of the converter, so we know the lowest limit of our converter for detecting the fluorescein concentration is 6.25uM.


The next curve is the fluorescein concentration corresponded to the fluorescence in order to check whether two curves have similar positive correlation.


pH level

We chose a test paper with a range from pH 5.0 to pH 8.0. For example, when the color of the test paper changes from red to green, the pH value changes from 5 to 8. Color detection is used to to detect the test paper and register the RGB value. The RGB value allows us to measure the pH value.


Transmission

Five values (two for the fluorescence intensity, three for the RGB values) we received will be transmitted from Arduino to the APP by our Bluetooth, HC-06.

Future Plan

For the fluorescence intensity, we hope we can detect the larger range of the fluorescence intensity by the circuit we designed so that the accuracy can be improved.
For the pH level, we hope we can plot the suitable curve or update our App to know the precise pH value in the future.

Materials

  • Arduino Uno
  • Slides
  • Test paper
  • 485nm monochromatic light
  • 510nm filter
  • Lens
  • TSL 235R
  • TCS3200
  • HC-06
  • Small Breadbroad
  • Male to Female DuPont Breadboard Jumper Wires