Difference between revisions of "Team:CCU Taiwan/Device detection"

 
(5 intermediate revisions by 2 users not shown)
Line 108: Line 108:
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li>
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
 +
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
  
Line 170: Line 172:
 
</li>
 
</li>
  
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Medals">Medals</a></li>
 
 
 
</ul>
 
</ul>
Line 189: Line 191:
 
<ul>
 
<ul>
 
<li>
 
<li>
−
<a href="#Devices">Device detection</a>
+
<a href="#Detection">Detection</a>
 
<ul>
 
<ul>
−
<li><a href="#light">Light intensity</a></li>
+
<li><a href="#Fluorescence">Fluorescence detection</a></li>
−
<li><a href="#pH">pH value</a></li>
+
<li><a href="#Optical">Optical system</a></li>
−
<li><a href="#Materials">Materials</a></li>
+
<li><a href="#The">Monochromatic light</a></li>
−
+
<li><a href="#Experiment">Experiment</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
  
 +
<li>
 +
<a href="#pH">pH level</a>
 +
 +
</li>
 +
 +
<li>
 +
<a href="#Transmission">Transmission</a>
 +
 +
</li>
 +
 +
<li>
 +
<a href="#Future">Future Plan</a>
 +
 +
</li>
 +
 +
<li>
 +
<a href="#Materials">Materials</a>
 +
 +
</li>
  
 
</ul>
 
</ul>
Line 209: Line 230:
  
  
−
<div id="Devices">
+
<div id="Detection">
 
<h2>Detection</h2>
 
<h2>Detection</h2>
  
Line 218: Line 239:
 
</div>
 
</div>
  
 +
 +
<div id="Fluorescence">
 +
<div class="aaa"></div>
 
<h3>Fluorescence detection</h3>
 
<h3>Fluorescence detection</h3>
 
<p>
 
<p>
Line 225: Line 249:
  
 
</p>
 
</p>
 +
</div>
  
−
 
+
<div id="Optical">
 +
<div class="aaa"></div>
 
<h3>Optical system</h3>
 
<h3>Optical system</h3>
 
<p>
 
<p>
Line 233: Line 259:
  
 
<img src="https://static.igem.org/mediawiki/2017/4/4b/FFF.jpg" style="display:block; margin:auto;"><br/>
 
<img src="https://static.igem.org/mediawiki/2017/4/4b/FFF.jpg" style="display:block; margin:auto;"><br/>
 +
</div>
  
  
 +
<div id="The">
 +
<div class="aaa"></div>
 
<h3>The monochromatic light</h3>
 
<h3>The monochromatic light</h3>
 
<p>
 
<p>
Line 247: Line 276:
  
 
<img src="https://static.igem.org/mediawiki/2017/0/0c/DR4.png" style="display:block; margin:auto;"><br/>
 
<img src="https://static.igem.org/mediawiki/2017/0/0c/DR4.png" style="display:block; margin:auto;"><br/>
 +
</div>
 +
  
 +
<div id="Experiment">
 +
<div class="aaa"></div>
 
<h3>Experiment</h3>
 
<h3>Experiment</h3>
 
<p>
 
<p>
Line 260: Line 293:
  
 
<img src="https://static.igem.org/mediawiki/2017/a/a7/DR5.png" style="display:block; margin:auto;"><br/>
 
<img src="https://static.igem.org/mediawiki/2017/a/a7/DR5.png" style="display:block; margin:auto;"><br/>
 +
</div>
  
 +
<div id="pH">
 
<h2>pH level</h2>
 
<h2>pH level</h2>
  
Line 269: Line 304:
  
 
<img src="https://static.igem.org/mediawiki/2017/c/ca/Ph_v.png" style="display:block; margin:auto;"><br/>
 
<img src="https://static.igem.org/mediawiki/2017/c/ca/Ph_v.png" style="display:block; margin:auto;"><br/>
 +
</div>
 +
  
 +
<div id="Transmission">
 
<h2>Transmission</h2>
 
<h2>Transmission</h2>
 
<p>
 
<p>
 
Five values (two for the fluorescence intensity, three for the RGB values) we received will be transmitted from Arduino to the APP by our Bluetooth, HC-06.
 
Five values (two for the fluorescence intensity, three for the RGB values) we received will be transmitted from Arduino to the APP by our Bluetooth, HC-06.
 
</p>
 
</p>
 +
</div>
  
 +
<div id="Future">
 
<h2>
 
<h2>
 
Future Plan
 
Future Plan
Line 280: Line 320:
  
 
<p>
 
<p>
−
For the fluorescence intensity, we hope we can increase the range of the fluorescence intensity through designing circuit. So the accuracy can be improved.
+
For the fluorescence intensity, we hope we can detect the larger range of the fluorescence intensity by the circuit we designed so that the accuracy can be improved.
 
<br/>
 
<br/>
−
For the pH level, users need to distinguish the color of pH value by eyes and enter to the App now. In the future, we hope we can plot the suitable curve or develop our App to know the pH value.
+
For the pH level, we hope we can plot the suitable curve or update our App to know the precise pH value in the future.
  
 
</p>
 
</p>
 +
</div>
 +
 +
<div id="Materials">
  
 
<h2>Materials</h2>
 
<h2>Materials</h2>
 
<p>
 
<p>
−
Arduino Uno<br/>
+
<li> Arduino Uno<br/>
−
Slides<br/>
+
<li> Slides<br/>
−
Test paper<br/>
+
<li> Test paper<br/>
−
485nm monochromatic light<br/>
+
<li> 485nm monochromatic light<br/>
−
510nm filter <br/>
+
<li> 510nm filter <br/>
−
Lens<br/>
+
<li> Lens<br/>
−
TSL 235R<br/>
+
<li> TSL 235R<br/>
−
TCS3200<br/>
+
<li> TCS3200<br/>
−
HC-06<br/>
+
<li> HC-06<br/>
−
Small Breadbroad<br/>
+
<li> Small Breadbroad<br/>
−
Male to Female DuPont Breadboard Jumper Wires
+
<li> Male to Female DuPont Breadboard Jumper Wires
  
  
 
</p>
 
</p>
 +
</div>
  
  
Line 337: Line 381:
  
 
<!-- Contact -->
 
<!-- Contact -->
−
<section class="contact">
+
−
+
−
<ul class="icons">
+
−
 
+
−
<li>
+
−
<a href="https://www.facebook.com/ccuigemteam" target="_blank">
+
−
<img src="https://static.igem.org/mediawiki/2016/2/2f/T--Harvard_BioDesign--images_facebook01.png"alt="Facebook Logo" style="width:51px;height:51px;">
+
−
</a>
+
−
</li>
+
−
 
+
−
              <li>
+
−
              <a href="emailto:ccu.igem.2017@gmail.com">
+
−
              <img src="https://static.igem.org/mediawiki/2016/e/e2/T--Harvard_BioDesign--images_gmail01.png" alt="Email Logo" style="width:51px;height:51px;">
+
−
              </a>
+
−
              </li>
+
−
 
+
−
 
+
−
             
+
−
 
+
−
</ul>
+
−
</section>
+
  
 
<!-- Copyright -->
 
<!-- Copyright -->
 
<div class="copyright">
 
<div class="copyright">
−
<ul class="menu">
+
−
<li>&#169; 2017 CCU Taiwan iGEM</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
+
−
</ul>
+
 
 
  
 
</div>
 
</div>
−
 
+
<img src="https://static.igem.org/mediawiki/2017/f/fb/Ccu.jpg" width="400" height="120" style="margin-top: 5em;
 +
    margin-right: 5em;">
 +
<img src="https://static.igem.org/mediawiki/2017/4/48/%E7%BF%94%E5%AE%8F-logo.jpg" width="400" height="120">
 
</div>
 
</div>
 
</div>
 
</div>
Line 373: Line 397:
  
 
</div>
 
</div>
 +
 +
<!-- Scripts -->
  
  
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 06:00, 15 December 2017

No Sidebar - Helios by HTML5 UP

Detection

We used Arduino UNO with a light frequency converter to detect the light intensity of GFP, and a color detector to distinguish the color of the test paper.


Fluorescence detection

First, we set the light frequency converter(TSL235R) to measure the light intensity of GFP emitted from our CSP and Lactic acid detectors.
Secondly, we created a standard curve by using a measuring instrument in our laboratory to detect the light intensity emitted from known concentrations of CSP and Lactic acid precisely.
Finally, we compared the light intensity detected by our device with the standard curve to determine the concentration of CSP or Lactic acid in the sample.

Optical system

For the detection of the fluorescence emitted by the bacteria after it combines with the targets, a monochromatic light(485nm) was set to excite the fluorescent protein. An optical sensor(TSL235R), two lens and a longpass filter(510nm) were placed to filter the unwanted light and focus the rest onto the optical sensor to detect its fluorescence intensity.


The monochromatic light

The GFP we were using was GFPmut3. Picture A shows the excitation maxima of GFPmut3 is 501nm, and picture B shows the emission maxima is 511nm.


The purple line is the 510nm filter what we use, but it’s starting point is not 510nm exactly. So we chose the 485nm light to avoid unwanted light detection.


Experiment

The following curve is the fluorescein concentration corresponded to the frequency of the converter, so we know the lowest limit of our converter for detecting the fluorescein concentration is 6.25uM.


The next curve is the fluorescein concentration corresponded to the fluorescence in order to check whether two curves have similar positive correlation.


pH level

We chose a test paper with a range from pH 5.0 to pH 8.0. For example, when the color of the test paper changes from red to green, the pH value changes from 5 to 8. Color detection is used to to detect the test paper and register the RGB value. The RGB value allows us to measure the pH value.


Transmission

Five values (two for the fluorescence intensity, three for the RGB values) we received will be transmitted from Arduino to the APP by our Bluetooth, HC-06.

Future Plan

For the fluorescence intensity, we hope we can detect the larger range of the fluorescence intensity by the circuit we designed so that the accuracy can be improved.
For the pH level, we hope we can plot the suitable curve or update our App to know the precise pH value in the future.

Materials

  • Arduino Uno
  • Slides
  • Test paper
  • 485nm monochromatic light
  • 510nm filter
  • Lens
  • TSL 235R
  • TCS3200
  • HC-06
  • Small Breadbroad
  • Male to Female DuPont Breadboard Jumper Wires