Difference between revisions of "Team:CCU Taiwan/HP/Gold Integrated"

 
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<header>
 
<header>
<h1><a href="https://2017.igem.org/Team:CCU_Taiwan" id="logo">Gold Integrated</a></h1>
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<h1><a href="https://2017.igem.org/Team:CCU_Taiwan" id="logo">Integrated & Gold</a></h1>
  
  
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<nav id="nav">
 
<nav id="nav">
 
<ul>
 
<ul>
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                                <li><a href="https://2017.igem.org/Team:CCU_Taiwan">Home</a></li>
  
  
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<a href="#">Project</a>
 
<a href="#">Project</a>
 
<ul>
 
<ul>
<li><a href="#">Description</a></li>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
 
                                                                                 <li>
 
                                                                                 <li>
<a href="#">Biosensor</a>
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<a href="https://2017.igem.org/Team:CCU_Taiwan/Design">Biosensor</a>
 
<ul>
 
<ul>
<li><a href="#">CSP detector</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Design#CSP">CSP detector</a></li>
<li><a href="#">Lactate detector</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Design#Lactate">Lactate detector</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
<li><a href="#">Modeling</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Test_paper">Test paper</a></li>
<li><a href="#">Experiment</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
 
 
<li><a href="Results">Result</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
<li><a href="#">Future perspective</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li>
<li><a href="#">Safty</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li>
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
 +
 
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
 +
 
 
</ul>
 
</ul>
 
</li>
 
</li>
  
 
<li>
 
<li>
<a href="#">Dry lab</a>
+
 
 +
<a href="#">Hardware</a>
 
<ul>
 
<ul>
                                                                                <li>
+
 
<a href="#">Hardware</a>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Hardware">Hardware overview</a></li>
<ul>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Device_design">Device design</a></li>
<li><a href="#">Device</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Device_detection">Device detection</a></li>
<li><a href="#">Detector</a></li>
+
</ul>
</ul>
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</li>
 
</li>
  
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<a href="#">Software</a>
 
<a href="#">Software</a>
 
<ul>
 
<ul>
<li><a href="#">IOT system</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/IOT system">IOT system</a></li>
<li><a href="#">APP</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/APP">APP</a></li>
                                                                                                 <li><a href="#">Machine learning</a></li>
+
                                                                                                 <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Machine_learning">Machine learning</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
</ul>
+
 
</li>
 
</li>
 
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Model">Modeling</a></li>
  
 
<li>
 
<li>
 
<a href="#">Human practice</a>
 
<a href="#">Human practice</a>
 
<ul>
 
<ul>
<li><a href="#">Silver</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Human_Practices">Human practice overview</a></li>
<li><a href="#">Gold and Integrated</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/HP/Silver">Silver HP</a></li>
<li><a href="#">Engagemant</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/HP/Gold_Integrated">Integrate & Gold</a></li>
<li><a href="#">Entrepreneurship</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Engagement">Public engagemant</a></li>
</ul>
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</li>
+
 
+
<li>
+
<a href="#">Part</a>
+
<ul>
+
<li><a href="#">Basic part</a></li>
+
<li><a href="#">Composite part</a></li>
+
 
 
 
</ul>
 
</ul>
 
</li>
 
</li>
 
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
 
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
 
<li>
 
<li>
<a href="#">Notebook</a>
+
<a href="#">Parts</a>
 
<ul>
 
<ul>
<li><a href="#">Schedule</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
<li><a href="#">Protocol</a></li>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Basic_Part">Basic parts</a></li>
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Composite_Part">Composite parts</a></li>
 
 
 
</ul>
 
</ul>
 
</li>
 
</li>
 +
  
  
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<a href="#">Team</a>
 
<a href="#">Team</a>
 
<ul>
 
<ul>
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Member">Member</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Members">Team members</a></li>
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Collaborations">Collaborations</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Collaborations">Collaborations</a></li>
<li><a href="#">Attribution</a></li>
 
 
</ul>
 
</li>
 
 
 
<li>
 
<a href="#">Judging</a>
 
<ul>
 
<li><a href="#">Gold</a></li>
 
<li><a href="#">Silver</a></li>
 
<li><a href="#">Bronze</a></li>
 
 
 
 
</ul>
 
</ul>
 
</li>
 
</li>
  
 
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Medals">Medals</a></li>
 
+
 
+
<li><a href="no-sidebar.html">Q&A</a></li>
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</ul>
 
</ul>
 
</nav>
 
</nav>
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<nav class="toc_nav" id="toc_show">
 
<nav class="toc_nav" id="toc_show">
 
<ul>
 
<ul>
 +
                <li>
 +
<a href="#Gold">Gold and integrated</a>
 +
</li>
 +
 
<li>
 
<li>
<a href="#Fluorescein">Fluorescein curve</a>
+
<a href="#Survey">Survey</a>
<ul>
+
 
<li><a href="#Fluorescein-Plate-reader">Plate reader</a></li>
+
<li><a href="#Fluorescein-Material">Material</a></li>
+
<li><a href="#Fluorescein-Method">Method</a></li>
+
<li><a href="#Fluorescein-Data-result">Data result</a></li>
+
</ul>
+
 
</li>
 
</li>
 +
 
<li>
 
<li>
<a href="#OD600">OD600 Reference point</a>
+
<a href="#Communicate">Communicate</a>
      <ul>
+
 
<li><a href="#OD600-Plate-reader">Plate reader</a></li>
+
<li><a href="#OD600-Material">Material</a></li>
+
<li><a href="#OD600-Method">Method</a></li>
+
<li><a href="#OD600-Data-result">Data result</a></li>
+
      </ul>
+
 
</li>
 
</li>
  
 
<li>
 
<li>
<a href="#Cell">Cell measure</a>
+
<a href="#Improve">Education engagement</a>
      <ul>
+
 
<li><a href="#Cell-Material">Material</a></li>
+
<li><a href="#Cell-Method">Method</a></li>
+
<li><a href="#Cell-Data-result">Data result</a></li>
+
      </ul>
+
 
</li>
 
</li>
  
 +
                <li>
 +
<a href="#Pro">Professional engagement</a>
 +
 +
</li>
 +
 +
 +
                <li>
 +
<a href="#Integrated">Integrated</a>
 +
 +
</li>
  
 
</ul>
 
</ul>
 
   <svg class="toc-marker" width="200" height="200" xmlns="http://www.w3.org/2000/svg">
 
   <svg class="toc-marker" width="200" height="200" xmlns="http://www.w3.org/2000/svg">
     <path stroke="#444" stroke-width="3" fill="transparent" stroke-dasharray="0, 0, 0, 1000" stroke-linecap="round" stroke-linejoin="round" transform="translate(-0.5, -0.5)" />
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     <path stroke="#00dbff" stroke-width="3" fill="transparent" stroke-dasharray="0, 0, 0, 1000" stroke-linecap="round" stroke-linejoin="round" transform="translate(-0.5, -0.5)" />
 
   </svg>
 
   </svg>
 
</nav>
 
</nav>
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   <section>
 
   <section>
  
 +
<div id="Gold">
 +
<h2>Gold and integrated</h2>
  
<div id="Fluorescein">
+
<p>
<h2>Fluorescein Fluorescence standard curve</h2>
+
We made a series of survey to observe the users willingness, to see whether they meet the need of consumers.<br/>
<div class="aaa"></div>
+
We sought for critiques frequently, kept modifying our product to so that user can use them better
 +
</p>
 
</div>
 
</div>
  
<div id="Fluorescein-Plate-reader">
+
<div id="Survey">
 +
<h2>Survey</h2>
 +
<h3>1. Online product form survey and PMR survey:</h3>
 +
<p>In order to know the willingness of using our product in Taiwan and foreign regions, we conducted a online product form questionnaire survey and PMR (Potential Market Research). Next, we went on the streets and asked random people for their thoughts about our project in order to actually learn customers needs.<br/>
 +
Total samples:<br/>
 +
Taiwan: 187 persons<br/>
 +
Foreign: 70 person<br/>
 +
The survey results are as follows:
 +
</p>
  
<h3>Plate reader</h3>
+
<h3>Taiwan</h3><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/9/94/G1.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/8/8e/G2.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/7/7d/G3.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/6/65/G4.jpg" style="display:block; margin:auto;"><br/>
  
    <p>
 
      microplate reader FLUOstar Omega</br>
 
emission filter: 520 nm</br>
 
excitation filter: 485 nm
 
    </p>
 
<div class="aaa"></div>
 
</div>
 
 
 
<div id="Fluorescein-Material">
 
  
<h3>Material</h3>
+
<h3>Foreign</h3><br/>
<p>
+
<img src="https://static.igem.org/mediawiki/2017/3/3d/G5.jpg" style="display:block; margin:auto;"><br/>
Fluorescein sodium salt</br>
+
<img src="https://static.igem.org/mediawiki/2017/8/89/G6.jpg" style="display:block; margin:auto;"><br/>
1xPBS</br>
+
<img src="https://static.igem.org/mediawiki/2017/c/cd/G7.jpg" style="display:block; margin:auto;"><br/>
Tissue culture testplate (black with flat bottom)
+
<img src="https://static.igem.org/mediawiki/2017/4/4d/G8.jpg" style="display:block; margin:auto;"><br/>
</p>
+
<img src="https://static.igem.org/mediawiki/2017/5/51/G9.jpg" style="display:block; margin:auto;"><br/>
<div class="aaa"></div>
+
<img src="https://static.igem.org/mediawiki/2017/5/5d/G10.jpg" style="display:block; margin:auto;"><br/>
</div>
+
  
<div id="Fluorescein-Method">
 
  
                <h3>Method</h3>
+
 
+
<h3>Summary</h3>
<ol><li>Prepare fluorescein stock solution</li></ol>
+
 
<p>
 
<p>
1. Spin down fluorescein stock tube to make sure pellet is at the bottom of tube.</br>  
+
From the graphs above, we can see that children from 7-12 years old will have a school dentist to check their oral condition regularly. By knowing so, we still asked primary school staffs and 36 parents for their willingness of purchasing. It turned out that nine schools and 93% of the parents were willing to use this product.<br/>
2. Prepare 2x fluorescein stock solution (100 μM) by resuspending fluorescein in 1 mL of 1xPBS. </br>
+
However, when we asked the same question to the parents outside the school. Most of them considered our product unnecessary and regularly school check is enough. The following will explain the reasons why parents don’t purchase Carindex for their children.<br/>
3. Dilute the 2x fluorescein stock solution with 1xPBS to make a 1x fluorescein solution and resulting concentration of fluorescein  stock solution 50 μM </br></br>
+
For children at about 16 years old, they would prefer going to dentist on their own. So parents don’t need to buy the products.<br/>
 +
As for 19 years old children, many of them go to other city’s university, have their own jobs, etc. So parents don’t need to buy the products for them.<br/>
 +
We found that approximately 73% of people don’t want to use this product, because they think going to dentist is enough, and the product is too expensive.<br/>
 +
On the other hand, foreign people have higher willingness to use this product at home, because going to dentist would cost more, compared to this product price.
 
</p>
 
</p>
<ol><li>Serial dilutions</li></ol>
+
</div>
 +
 
 +
<div id="Communicate">
 +
<h2>Communicate with public</h2>
 +
 
 +
 
 +
 
 +
<h3>Product pamphlet</h3>
 +
 
 
<p>
 
<p>
1. Add 100 μl of PBS into wells A2, B2, C2, D2....A12, B12, C12, D12</br>
+
In order to let the public understand our product in an easy way, we released pamphlets in order to do so. In the pamphlet, we briefly introduced how our product can be used, the technology that is applied and also let them know that Carindex is a genetic engineered product. Then we introduce the kill switch in our gene circuit design to let them know the concept of biosafety, at the same time, promoting the safety of our product.
2. Add 200 μl of fluorescein 1x stock solution into A1, B1, C1, D1</br>
+
3. Transfer 100 μl of fluorescein stock solution from A1 into A2. </br>
+
4. Mix A2 by pipetting up and down 3x and transfer 100 μl into A3. </br>
+
5. Mix A3 by pipetting up and down 3x and transfer 100 μl into A4. </br>
+
6. Mix A4 by pipetting up and down 3x and transfer 100 μl into A5. </br>
+
7. Mix A5 by pipetting up and down 3x and transfer 100 μl into A6. </br>
+
8. Mix A6 by pipetting up and down 3x and transfer 100 μl into A7. </br>
+
9. Mix A7 by pipetting up and down 3x and transfer 100 μl into A8. </br>
+
10. Mix A8 by pipetting up and down 3x and transfer 100 μl into A9. </br>
+
11. Mix A9 by pipetting up and down 3x and transfer 100 μl into A10.</br>
+
12. Mix A10 by pipetting up and down 3x and transfer 100 μl into A11. </br>
+
13. Mix A11 by pipetting up and down 3x and transfer 100 μl into liquid waste.
+
  (Caution: Do not to continue serial dilution into column 12.)</br>
+
 
</p>
 
</p>
<ol><li>repeat serial dilute for Row B、D、E</strong></li></ol>
+
 
<ol><li>Measure fluorescence of all samples in all standard measurement modes in instrument Record the data in your notebook</strong></li></ol>
+
<embed src="https://static.igem.org/mediawiki/2017/6/62/%E5%B0%8F%E6%9B%B86.0_5.pdf" width="100%" height="600px">
<ol><li>Import data into Excel (fluorescein standard curve tab ) Sheet_1 provided</li></ol>
+
+
 
<br/><br/>
 
<br/><br/>
<div class="aaa"></div>
 
</div>
 
<div id="Fluorescein-Data-result">
 
  
<h3>Data result</h3>
 
<br/>
 
<img src="https://static.igem.org/mediawiki/2017/8/85/FFs_1.png" style="display:block; margin:auto;"><br/><br/>
 
<img src="https://static.igem.org/mediawiki/2017/a/a4/FFs_2.png" style="display:block; margin:auto;"><br/><br/>
 
<img src="https://static.igem.org/mediawiki/2017/5/5c/FFs_3.png" style="display:block; margin:auto;"><br/>
 
</div>
 
</section>
 
  
<section>
 
  
 +
<h3>Public booth activity</h3>
 +
<p>
 +
In order to enable our product to actually reach the public, we communicate closely with the public and asked them for feedbacks. From these feedbacks, we modified our product so that it can be more intimate to social needs. We also cooperated with a kumquat farm which is a famous tourist attraction from Taiwan Chiayi for holding a booth. In the crowd booth, we showed our prototype and the product pamphlet to the public, introduced our project including knowledge regrading dental health care, the function of our product and the follow-up benefits of it. Many parents and children showed a great interest in Carindex after the introduction. We had received a lot of feedbacks from the public, and grasp a better understanding to the cavity problem that they had. These precious feedbacks helped us to improve the Carindex and made it in line with the user needs!
 +
</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2017/a/a9/Eng1.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/9/99/Eng2.jpg" style="display:block; margin:auto;"><br/>
  
<div id="OD600">
 
<h2>OD600 Reference point</h2>
 
<div class="aaa"></div>
 
 
</div>
 
</div>
  
 +
<div id="Improve">
 +
<h2>Education engagement</h2>
  
<div id="OD600-Plate-reader">
 
  
<h3>Plate reader</h3>
+
<h3>1. Biology Experiment:</h3>
 +
<p>
 +
To let students learn more about what we are doing, we designed a series of fun and easy DNA purification experiment for them. After the activities, we still remembered that many of them were so excited at the DNA that they purified. They said that they enjoyed it a lot and think that such activities are very awesome, hoping to have more opportunities to participate the same kind of things in the future.
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2017/d/d0/Eng4.jpg" style="display:block; margin:auto;"><br/>
  
    <p>
+
 
Thermo Scientific™ Multiskan™ FC Filter-based Microplate Photometer</br>
+
 
Filter: 595 nm</br>
+
<h3>2. Synthetic Biology Camp:</h3>
    </p>
+
<p>
<div class="aaa"></div>
+
We set up plans to promote synthetic biology right after the summer started. We went to universities and senior high schools, introduced iGEM and synthetic biology and our project to the students so that they can understand the biotechnology as well as the various aspects of the impact from it. Many students had concerns about genetic engineered products and the related issues. After knowing what we were doing, they asked us what if the genetic engineered bacteria we made broke out the device, how are we going to deal with it. We told them about the kill switch we designed, ease their concern; at the same time, bring the security concept of biotechnology to them. According to after-class feedback, before the class, most of the students had have the same concern about genetic engineered product. However, after class, most of them grasped a correct and better understanding of synthetic biology, genetic modification, and it security. This eased their concern and make them want to do the same thing in future.
 +
</p>
 +
<img src="https://static.igem.org/mediawiki/2017/4/45/Eng5.jpg" width="787" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/9/95/Eng6.jpg" style="display:block; margin:auto;"><br/>
 
</div>
 
</div>
 
 
<div id="OD600-Material">
 
  
<h3>Material</h3>
+
<div id="Pro">
<p>
+
<h2>Professional engagement</h2>
1 ml LUDOX</br>
+
mQH<sub>2</sub>O</br>
+
96 well cell culture plate (clear with flat-bottom)
+
</p>
+
<div class="aaa"></div>
+
</div>
+
  
<div id="OD600-Method">
 
  
                <h3>Method</h3>
 
  
 +
<h3>3. Dentist Collaboration:</h3>
 
<p>
 
<p>
1. Add 100 μl LUDOX into wells A1, B1, C1, D1 (or 1 mL LUDOX into cuvette)</br>
+
We visited a number of Taiwan Chiayi local dental clinic: 百利、沃爾、邱. From there, we understood the actual needs of the patient from the perspective of dentists and the patient actual oral cavity situation. We also had a better understanding of common habits of patients. The information helped us move one more step closer the public.
2. Add 100 μl of H<sub>2</sub>O into wells A2, B2, C2, D2 (or 1 mL H<sub>2</sub>O into cuvette)</br>
+
Afterward, we collaborated with them, asking them to help the team define our product internal data. We used the standard dental caries level of ICDAS (International Caries Detection and Assessment System) organization, which is recommend by dentists.
3. Measure absorbance 600 nm of all samples in all standard measurement modes in instrument</br>
+
 
4. Record the data in excel and Import data into Excel ( OD600 reference point tab ) Sheet_1 provided</br>
+
 
</p>
 
</p>
<div class="aaa"></div>
 
</div>
 
  
<div id="OD600-Data-result">
+
<img src="https://static.igem.org/mediawiki/2017/8/8b/Eng9.jpg" width="400" height="550" style="display:block; margin:auto; float:left;">
 +
<img src="https://static.igem.org/mediawiki/2017/b/ba/Eng10.jpg" width="350" height="550" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/e/ef/Eng11.jpg" style="display:block; margin:auto;"><br/>
 +
<img src="https://static.igem.org/mediawiki/2017/6/62/Eng12.jpg"  width="700" style="display:block; margin:auto;"><br/>
  
<h3>Data result</h3>
+
 
<br/>
+
 
<img src="https://static.igem.org/mediawiki/2017/5/5a/600_1.png" style="display:block; margin:auto;"><br/>
+
<br/><h3>Primary school collaboration</h3>
 +
<p>
 +
After contacting with the public, we found out that there are chances that parents might be willing to buy this product for their children. So we visited primary school in Chiayi, and asked that whether they want to cooperate with us by using this device at school but we found out that the school would ask the dentist examine the Taiwanese young children every three months. If one's oral condition was not healthy, the dentist would ask one to see the dentist again after school. However, the process where the dentist checked the children oral condition was often too long, causing many inconvenience. Therefore, the school said that if the device improved it accuracy, they would like do the cooperation.
 +
</p>
 
</div>
 
</div>
</section>
 
  
<section>
+
<div id="Integrated">
 +
<h2>Integrated</h2>
 +
<p>
 +
After having contact with many people, elementary school students and public booth, they showed interests in our product and expected us to continue developing it. They believed that Carindex would be a very good product in the future after we improved it. The suggestions from public showed like the follow.
 +
</p>
  
 +
<img src="https://static.igem.org/mediawiki/2017/1/11/Ccu_42.png" style="display:block; margin:auto;">
 +
<p>
 +
Some people they won’t use the product because of no demands, but people who are interested in our product said they are unwilling to use this product as well, the reasons are that it is too complicated to use or it cost too much. On the other hand, nurses who work for elementary schools told us that our end user are students, maybe we can make our product more easily to use. As receiving those advice, we had made the following improvements to Carindex.
 +
</p>
  
<div id="Cell">
 
<h2>Cell measure</h2>
 
<div class="aaa"></div>
 
</div>
 
  
 
 
<div id="Cell-Material">
 
  
<h3>Material</h3>
+
<h3>1. Appearance Improvement:</h3>
<p>
+
<p>
Competent cells ( Escherichia coli strain DH5α)</br>
+
When we managed to cooperate with the local elementary schools, they suggested that we can design the device more lovely so that it would attract young children and increase their willingness of using.
LB (Luria Bertani) media</br>
+
</p>
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 μg /mL)</br>
+
</dir>
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light)</br>
+
Incubator at 37°C</br>
+
1.5 ml eppendorf tubes for sample storage</br>
+
Ice bucket with ice</br>
+
Pipettes</br>
+
96 well plate(cell culture 96 well plate、tissue culture testplate)</br>
+
Devices (from InterLab Measurement Kit):</br>
+
1. Negative control(BBa_R0040)</br>
+
2. Positive control(J23151+B0032+E0040+B0010+B0012)</br>
+
3. Test Device 1: J23101+I13504</br>
+
4. Test Device 2: J23106+I13504</br>
+
5. Test Device 3: J23117+I13504</br>
+
6. Test Device 4: J23101+BCD2+E0040+B0015</br>
+
7. Test Device 5: J23106+BCD2+E0040+B0015</br>
+
8. Test Device 6: J23117+BCD2+E0040+B0015</br>
+
</p>
+
<div class="aaa"></div>
+
</div>
+
  
<div id="Cell-Method">
 
  
                <h3>Method</h3>
+
<h3>2. Consumptive Materials Improvement:</h3>
 +
<p>
 +
After we communicate the public, we got critique that the device manipulation is too complicate. They hope that it could be installed like an ink clip of printer. Therefore, we modified the consumptive materials design. Originally, three slides of consumptive materials would have to be loaded respectively, one after one. We simplified this process by building a holder to integrate 3 slides together. Now that our customers will only have to load 1 consumptive materials complex.
 +
</p>
  
 +
<img src="https://static.igem.org/mediawiki/2017/2/26/IMG_5615.JPG" style="display:block; margin:auto;"><br/>
 +
 +
 +
</dir>
 +
 +
 +
<h3>3. Material Changing:</h3>
 
<p>
 
<p>
1. Day 1 : Resuspended each plasmid in plate 7 and transform into Escherichia coli DH5α.</br>
+
“It’s too expensive”, said most of the public we interviewed. Hence, we changed the materials from iron to acrylic material to reduce the cost of the product.
&nbsp;(Transformation protocol is from iGEM)</br>
+
2. Day 2 : Pick 2 colonies from each of plate and inoculate it on 5-10 mL LB medium +Chloramphenicol.Grow the cells overnight (16-18 hours) at 37°C and 170 rpm.</br>
+
3. Day 3 : Set instrument to read OD600 (as OD calibration setting)and measure OD600 of the overnight cultures</br>
+
4. Dilute the cultures to a target OD 600 of 0.02 in 12 ml LB medium + Chloramphenicol in 50 mL falcon tube (covered with foil to block light).</br>
+
5. Incubate the cultures at 37°C and 170 rpm.</br>
+
6. Take 1000 μL samples of the cultures at 0, 2, 4, and 6 hours of incubation and place samples on ice</br>
+
7. 4 replicates of 100 uL samples were taken from each culture at 0, 2, 4, and 6 hours of incubation and placed in a 96 well plate for OD and fluorescence measurements using the setup described above</br>
+
 
</p>
 
</p>
<div class="aaa"></div>
+
</dir>
</div>
+
  
<div id="Cell-Data-result">
 
  
<h3>Data result</h3>
+
<h3>4. Extra Germination Space:</h3>
<br/>
+
<p>
<img src="https://static.igem.org/mediawiki/2017/3/30/Cell_1.png" style="display:block; margin:auto;"><br/><br/>
+
When we visited the local primary schools, the health center staffs provided some advice on our product. Since the germination process of the bacteria on the consumptive materials were going to take a lot of time and there are about 30 students using it at the same time, the efficiency would be a concern. After hearing this, we enlarged Carindex, created an extra space to germinate 8 to 9 consumptive materials beforehand. With this improvement, the school were very interested in purchasing 3 Carindex. So we went with a plan that maybe each class can use Carindex in turns.
<img src="https://static.igem.org/mediawiki/2017/f/fe/Cell_2.png" style="display:block; margin:auto;"><br/>
+
 
 +
<img src="https://static.igem.org/mediawiki/2017/2/27/IMG_5654.JPG" style="display:block; margin:auto;"><br/>
 +
 
 +
</p>
 +
</dir>
 
</div>
 
</div>
 
</section>
 
</section>
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<!-- Contact -->
 
<!-- Contact -->
<section class="contact">
+
+
<ul class="icons">
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<li>
+
<a href="https://www.facebook.com/ccuigemteam" target="_blank">
+
<img src="https://static.igem.org/mediawiki/2016/2/2f/T--Harvard_BioDesign--images_facebook01.png"alt="Facebook Logo" style="width:51px;height:51px;">
+
</a>
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</li>
+
 
+
              <li>
+
              <a href="ccu.igem.2017@gmail.com">
+
              <img src="https://static.igem.org/mediawiki/2016/e/e2/T--Harvard_BioDesign--images_gmail01.png" alt="Email Logo" style="width:51px;height:51px;">
+
              </a>
+
              </li>
+
 
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              <li>
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              <a href="#" target="_blank">
+
              <img src="https://static.igem.org/mediawiki/2016/4/4e/T--Harvard_BioDesign--images_twitter01.png"alt="Twitter Logo" style="width:51px;height:51px;">
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              </a>
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              </li>
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+
 
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</ul>
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</section>
+
  
 
<!-- Copyright -->
 
<!-- Copyright -->
 
<div class="copyright">
 
<div class="copyright">
<ul class="menu">
+
<li>&#169; 2017 CCU Taiwan iGEM</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
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</ul>
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<img src="https://static.igem.org/mediawiki/2017/4/48/%E7%BF%94%E5%AE%8F-logo.jpg" width="400" height="120">
 
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Latest revision as of 13:56, 15 December 2017

No Sidebar - Helios by HTML5 UP

Gold and integrated

We made a series of survey to observe the users willingness, to see whether they meet the need of consumers.
We sought for critiques frequently, kept modifying our product to so that user can use them better

Survey

1. Online product form survey and PMR survey:

In order to know the willingness of using our product in Taiwan and foreign regions, we conducted a online product form questionnaire survey and PMR (Potential Market Research). Next, we went on the streets and asked random people for their thoughts about our project in order to actually learn customers needs.
Total samples:
Taiwan: 187 persons
Foreign: 70 person
The survey results are as follows:

Taiwan






Foreign








Summary

From the graphs above, we can see that children from 7-12 years old will have a school dentist to check their oral condition regularly. By knowing so, we still asked primary school staffs and 36 parents for their willingness of purchasing. It turned out that nine schools and 93% of the parents were willing to use this product.
However, when we asked the same question to the parents outside the school. Most of them considered our product unnecessary and regularly school check is enough. The following will explain the reasons why parents don’t purchase Carindex for their children.
For children at about 16 years old, they would prefer going to dentist on their own. So parents don’t need to buy the products.
As for 19 years old children, many of them go to other city’s university, have their own jobs, etc. So parents don’t need to buy the products for them.
We found that approximately 73% of people don’t want to use this product, because they think going to dentist is enough, and the product is too expensive.
On the other hand, foreign people have higher willingness to use this product at home, because going to dentist would cost more, compared to this product price.

Communicate with public

Product pamphlet

In order to let the public understand our product in an easy way, we released pamphlets in order to do so. In the pamphlet, we briefly introduced how our product can be used, the technology that is applied and also let them know that Carindex is a genetic engineered product. Then we introduce the kill switch in our gene circuit design to let them know the concept of biosafety, at the same time, promoting the safety of our product.



Public booth activity

In order to enable our product to actually reach the public, we communicate closely with the public and asked them for feedbacks. From these feedbacks, we modified our product so that it can be more intimate to social needs. We also cooperated with a kumquat farm which is a famous tourist attraction from Taiwan Chiayi for holding a booth. In the crowd booth, we showed our prototype and the product pamphlet to the public, introduced our project including knowledge regrading dental health care, the function of our product and the follow-up benefits of it. Many parents and children showed a great interest in Carindex after the introduction. We had received a lot of feedbacks from the public, and grasp a better understanding to the cavity problem that they had. These precious feedbacks helped us to improve the Carindex and made it in line with the user needs!



Education engagement

1. Biology Experiment:

To let students learn more about what we are doing, we designed a series of fun and easy DNA purification experiment for them. After the activities, we still remembered that many of them were so excited at the DNA that they purified. They said that they enjoyed it a lot and think that such activities are very awesome, hoping to have more opportunities to participate the same kind of things in the future.


2. Synthetic Biology Camp:

We set up plans to promote synthetic biology right after the summer started. We went to universities and senior high schools, introduced iGEM and synthetic biology and our project to the students so that they can understand the biotechnology as well as the various aspects of the impact from it. Many students had concerns about genetic engineered products and the related issues. After knowing what we were doing, they asked us what if the genetic engineered bacteria we made broke out the device, how are we going to deal with it. We told them about the kill switch we designed, ease their concern; at the same time, bring the security concept of biotechnology to them. According to after-class feedback, before the class, most of the students had have the same concern about genetic engineered product. However, after class, most of them grasped a correct and better understanding of synthetic biology, genetic modification, and it security. This eased their concern and make them want to do the same thing in future.



Professional engagement

3. Dentist Collaboration:

We visited a number of Taiwan Chiayi local dental clinic: 百利、沃爾、邱. From there, we understood the actual needs of the patient from the perspective of dentists and the patient actual oral cavity situation. We also had a better understanding of common habits of patients. The information helped us move one more step closer the public. Afterward, we collaborated with them, asking them to help the team define our product internal data. We used the standard dental caries level of ICDAS (International Caries Detection and Assessment System) organization, which is recommend by dentists.





Primary school collaboration

After contacting with the public, we found out that there are chances that parents might be willing to buy this product for their children. So we visited primary school in Chiayi, and asked that whether they want to cooperate with us by using this device at school but we found out that the school would ask the dentist examine the Taiwanese young children every three months. If one's oral condition was not healthy, the dentist would ask one to see the dentist again after school. However, the process where the dentist checked the children oral condition was often too long, causing many inconvenience. Therefore, the school said that if the device improved it accuracy, they would like do the cooperation.

Integrated

After having contact with many people, elementary school students and public booth, they showed interests in our product and expected us to continue developing it. They believed that Carindex would be a very good product in the future after we improved it. The suggestions from public showed like the follow.

Some people they won’t use the product because of no demands, but people who are interested in our product said they are unwilling to use this product as well, the reasons are that it is too complicated to use or it cost too much. On the other hand, nurses who work for elementary schools told us that our end user are students, maybe we can make our product more easily to use. As receiving those advice, we had made the following improvements to Carindex.

1. Appearance Improvement:

When we managed to cooperate with the local elementary schools, they suggested that we can design the device more lovely so that it would attract young children and increase their willingness of using.

2. Consumptive Materials Improvement:

After we communicate the public, we got critique that the device manipulation is too complicate. They hope that it could be installed like an ink clip of printer. Therefore, we modified the consumptive materials design. Originally, three slides of consumptive materials would have to be loaded respectively, one after one. We simplified this process by building a holder to integrate 3 slides together. Now that our customers will only have to load 1 consumptive materials complex.


3. Material Changing:

“It’s too expensive”, said most of the public we interviewed. Hence, we changed the materials from iron to acrylic material to reduce the cost of the product.

4. Extra Germination Space:

When we visited the local primary schools, the health center staffs provided some advice on our product. Since the germination process of the bacteria on the consumptive materials were going to take a lot of time and there are about 30 students using it at the same time, the efficiency would be a concern. After hearing this, we enlarged Carindex, created an extra space to germinate 8 to 9 consumptive materials beforehand. With this improvement, the school were very interested in purchasing 3 Carindex. So we went with a plan that maybe each class can use Carindex in turns.