Difference between revisions of "Team:CCU Taiwan/Hardware"

 
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
 
                                                                                 <li>
 
                                                                                 <li>
−
<a href="https://2017.igem.org/Team:CCU_Taiwan/Biosensor">Biosensor</a>
+
<a href="https://2017.igem.org/Team:CCU_Taiwan/Design">Biosensor</a>
 
<ul>
 
<ul>
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/CSP">CSP detector</a></li>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Design#CSP">CSP detector</a></li>
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Lactate">Lactate detector</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Design#Lactate">Lactate detector</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Test_paper">Test paper</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Test_paper">Test paper</a></li>
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Experiment">Experiment</a></li>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
 
 
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Result">Result</a></li>
+
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li>
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied Design</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li>
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
 +
 
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
 +
 
 
</ul>
 
</ul>
 
</li>
 
</li>
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<ul>
 
<ul>
  
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Hardware">Hardware Overview</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Hardware">Hardware overview</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Device_design">Device design</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Device_design">Device design</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Device_detection">Device detection</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Device_detection">Device detection</a></li>
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<a href="#">Human practice</a>
 
<a href="#">Human practice</a>
 
<ul>
 
<ul>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/HP_Overview">Human practice overview</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Human_Practices">Human practice overview</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/HP/Silver">Silver HP</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/HP/Silver">Silver HP</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/HP/Gold_Integrated">Integrate & Gold</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/HP/Gold_Integrated">Integrate & Gold</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Engagement">Public Engagemant</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Engagement">Public engagemant</a></li>
 
 
 
</ul>
 
</ul>
 
</li>
 
</li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Safty">Safty</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
 
<li>
 
<li>
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<a href="#">Part</a>
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<a href="#">Parts</a>
 
<ul>
 
<ul>
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<li><a href="#">Basic part</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
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<li><a href="#">Composite part</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Basic_Part">Basic parts</a></li>
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Composite_Part">Composite parts</a></li>
 
 
 
</ul>
 
</ul>
 
</li>
 
</li>
−
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li>
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<a href="#">Team</a>
 
<a href="#">Team</a>
 
<ul>
 
<ul>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Team_Member">Team Member</a></li>
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<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Members">Team members</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Collaborations">Collaborations</a></li>
 
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Collaborations">Collaborations</a></li>
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</li>
 
</li>
  
 +
<li><a href="https://2017.igem.org/Team:CCU_Taiwan/Medals">Medals</a></li>
 
 
 
</ul>
 
</ul>
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<ul>
 
<ul>
 
<li>
 
<li>
−
<a href="#Devices">Devices detection</a>
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<a href="#Devices">Overview</a>
−
<ul>
+
</li>
−
<li><a href="#light">Light intensity</a></li>
+
 
−
<li><a href="#pH">pH value</a></li>
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                <li>
−
<li><a href="#Materials">Materials</a></li>
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<a href="#adv">Advantages</a>
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+
</li>
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</ul>
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 +
                <li>
 +
<a href="#future">Future Plan</a>
 
</li>
 
</li>
  
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<article class="contents_nav">
 
<article class="contents_nav">
 
   <section>
 
   <section>
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<div id="Devices">
 
<div id="Devices">
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<h2>Hardware overview</h2>
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<h2>Overview</h2>
−
 
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<img src="https://static.igem.org/mediawiki/2017/0/04/Devices_01.JPG" style="display:block; margin:auto;">
 
<p>
 
<p>
−
We designed an inexpensive and easy to use fluorescence detection system with Arduino. By simply putting the sample to our container and put it in the device, four kinds of data from the same sample can be detected. What’s more, the inner components are designated to change easily, so that other iGemers can modified it to fit their purpose. <br/>
+
We want to design a mobile and low cost device which can measure the GFP secreted by engineered bacteria. We think that bio-sensor, such as GFP detection is a very promising field in the future.<br/>
−
Usually, bubbles occurred when the sample taken up increase the fluorescence detection error. However, our device took advantage of capillary phenomenon to get the sample to fix this bubble problem. <br/>
+
In addition, we believed that the freeze-dried test paper is a prospective synthetic biology application. By applying the functional bacteria on the test paper and freeze-dry it, the test paper along with the bacteria is easy for storage and can be preserved for a very long time. That’s why we want to innovate an integrated device for this.
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As for the optical system, we use a green filter, a led light, two convex lens and a photodiode. Those thing are inexpensive and successfully detect the fluorescence.  
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</p>
 
</p>
  
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</div>
 
</div>
  
−
<div id="light">
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<div id="adv">
−
<div class="aaa"></div>
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<h2>Advantage of our device</h2>
−
<h3>Detecting of the light intensity</h3>
+
<p>
 +
<ol　type="1">
 +
1. The inner components are designed to change easily, so that other iGemers can modified it to fit their purpose.<br/>
 +
2. Usually, bubbles occurred when the sample taken up increase the fluorescence detection error. However, our device took advantage of capillary phenomenon to get the sample to fix this bubble problem.<br/>
 +
3. Our device can detect multiple parameter with one sample because of the glass jig design.<br/>
 +
4. Our device is inexpensive because most of the materials are cheap and easy to manufacture.<br/>
 +
5. Our device is added some automation idea and form a IOT system.</li>
 +
</ol>
 +
</p>
 +
</div>
  
−
    <p>
 
−
      First, We select the light to frequency converter to receive the light intensity of GFP which is emitted from our CSP and Lactic acid,
 
−
and we collect the frequency from the output of converter for every detection.
 
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Second, we use the measuring instrument in the laboratory to detect accurately to know the light intensity emitted from our CSP and Lactic acid.<br/>
 
−
Finally, we model the curve for the light intensity and the frequency, so we can know the light intensity relatively when we collect the frequency from the converter.<br/>
 
−
The following curve is the light intensity of GFP corresponded to the frequency of the converter.<br/><br/>
 
−
<img src="https://static.igem.org/mediawiki/2017/d/d4/F_to_d.png" style="display:block; margin:auto;"><br/>
 
  
−
    </p>
+
<div id="future">
 +
<h2>Future Plan</h2>
 +
<p>
 +
Because of the limited of time, this prototype may not achieve our perfect expectations but our device had moved a big forward step for doing an integrated device. Like optical system, bacteria germination system, sample taken up system and so on.
 +
In the future, we will mainly focus on improving our Carindex self-feedback system.
 +
</p>
 
</div>
 
</div>
  
−
 
+
<h3>Please click here for more detail about the technology</h3>
 +
<p>
  
  
 +
<ol><li><a class="link" href='https://2017.igem.org/Team:CCU_Taiwan/Device_design' style="display: block;">Device design</a></ol></li>
 +
<ol><li><a class="link" href='https://2017.igem.org/Team:CCU_Taiwan/Device_detection' style="display: block;">Device detection</a></ol></li>
 +
<ol><li><a class="link" href='https://2017.igem.org/Team:CCU_Taiwan/Demonstrate#Non' style="display: block;">Demo</a></ol></li>
  
  
−
<div id="pH">
+
</p>
−
<div class="aaa"></div>
+
 
−
<h3>Detecting of the pH value</h3>
+
  
−
    <p>
 
−
We choose the test paper changing its color from pH5.0 to pH8.0, for example, when the color of the test paper changing from red to green, the pH valut changing from 5 to 8,
 
−
and then use the color detector to detect the test paper and collect the value of RGB, so we can know the pH value after we draw the curve between the value of RGB and the pH value.<br/>
 
−
<br/>
 
−
<img src="https://static.igem.org/mediawiki/2017/c/ca/Ph_v.png" style="display:block; margin:auto;"><br/>
 
  
−
Final, these five values (two for the light intensity, and three for the values of Red, Green, and Blue ) we receved will be transmitted from Arduino by our Bluetooth, HC-06, to the APP.
 
−
    </p>
 
−
<br/>
 
−
</div>
 
  
  
  
−
<div id="Materials">
 
−
<div class="aaa"></div>
 
−
<h3>Materials</h3>
 
−
<p>
 
−
Arduino Uno
 
−
Test paper<br/>
 
−
TSL 235R<br/>
 
−
510nm Filter<br/>
 
−
485nm LED<br/>
 
−
TCS3200<br/>
 
−
HC-06<br/>
 
−
Convex lens<br/>
 
−
Small Breadbroad<br/>
 
−
Male to Female DuPont Breadboard Jumper Wires
 
  
−
</p>
 
  
−
</div>
 
 
</section>
 
</section>
  
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<!-- Contact -->
 
<!-- Contact -->
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<section class="contact">
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−
+
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<ul class="icons">
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−
<li>
+
−
<a href="https://www.facebook.com/ccuigemteam" target="_blank">
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−
<img src="https://static.igem.org/mediawiki/2016/2/2f/T--Harvard_BioDesign--images_facebook01.png"alt="Facebook Logo" style="width:51px;height:51px;">
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</a>
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</li>
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−
 
+
−
              <li>
+
−
              <a href="emailto:ccu.igem.2017@gmail.com">
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−
              <img src="https://static.igem.org/mediawiki/2016/e/e2/T--Harvard_BioDesign--images_gmail01.png" alt="Email Logo" style="width:51px;height:51px;">
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              </a>
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</ul>
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</section>
+
  
 
<!-- Copyright -->
 
<!-- Copyright -->
 
<div class="copyright">
 
<div class="copyright">
−
<ul class="menu">
+
−
<li>&#169; 2017 CCU Taiwan iGEM</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
+
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</ul>
+
 
 
  
 
</div>
 
</div>
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<img src="https://static.igem.org/mediawiki/2017/f/fb/Ccu.jpg" width="400" height="120" style="margin-top: 5em;
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    margin-right: 5em;">
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<img src="https://static.igem.org/mediawiki/2017/4/48/%E7%BF%94%E5%AE%8F-logo.jpg" width="400" height="120">
 
</div>
 
</div>
 
</div>
 
</div>
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</div>
 
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<!-- Scripts -->
  
  
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 05:57, 15 December 2017

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Overview

We want to design a mobile and low cost device which can measure the GFP secreted by engineered bacteria. We think that bio-sensor, such as GFP detection is a very promising field in the future.
In addition, we believed that the freeze-dried test paper is a prospective synthetic biology application. By applying the functional bacteria on the test paper and freeze-dry it, the test paper along with the bacteria is easy for storage and can be preserved for a very long time. That’s why we want to innovate an integrated device for this.


Advantage of our device

1. The inner components are designed to change easily, so that other iGemers can modified it to fit their purpose.
2. Usually, bubbles occurred when the sample taken up increase the fluorescence detection error. However, our device took advantage of capillary phenomenon to get the sample to fix this bubble problem.
3. Our device can detect multiple parameter with one sample because of the glass jig design.
4. Our device is inexpensive because most of the materials are cheap and easy to manufacture.
5. Our device is added some automation idea and form a IOT system.

Future Plan

Because of the limited of time, this prototype may not achieve our perfect expectations but our device had moved a big forward step for doing an integrated device. Like optical system, bacteria germination system, sample taken up system and so on. In the future, we will mainly focus on improving our Carindex self-feedback system.

Please click here for more detail about the technology

  1. Device design
  1. Device detection
  1. Demo