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| − | Template Page | + | AND Gate |
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| | ==Overview== | | ==Overview== |
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| − | | + | In the design of our <i>Campylobacter</i> biosensor we decided to employ a genetic logic circuit. This would require the presence of two transcriptional input signals from <i>Campylobacter</i>-associated molecules before an output signal in the form of GFP fluorescence could be produced. We aimed to build and characterise two genetic AND-gates; one based on a split-GFP fluorescence system; and the other based on a splitting the <i>Enterobacteria</i> phage T7 RNA polymerase. We wished to quantify the responsiveness of both AND-gates with well-characterised small-molecule regulated promoters from the iGEM registry, and then proceed to utilising our own <i>Campylobacter</i> sensing transcriptional systems to drive the AND-gate biosensor. Both split-GFP and split-T7 RNAP gates were constructed from BioBrick parts and tested, however DNA sequence errors appear to have prevented the proper function of either system. |
| − | <ref>Kiliç, A. O., Pavlova, S. I., Ma, W. G. & Tao, L. 1996. Analysis of <i>Lactobacillus</i> phages and bacteriocins in American dairy products and characterization of a phage isolated from yogurt. Appl Environ Microbiol, 62, 2111-6.</ref> | + | |
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| − | ==Aims==
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| − | *Aim 1
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| − | **Sub-aim 1
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| − | **Sub-aim 2
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| − | *Aim 2
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| − | **Sub-aim 1
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| − | **Sub-aim 2
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| − | ==Materials and Methods==
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| − | ===Condition set up===
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| − | ===Sample preparation===
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| − | <small> | + | |
| − | *1
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| − | *2
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| − | *3
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| − | </small> | + | |
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| − | {{GlasgowWikiImage|image=Glasgow2017_caption_image1.JPG|caption=<b>Table 1:</b> Optical density analysis of <i>S. thermophilus</i> growth}}
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| − | ==Results and Discussion==
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| − | ==Outlook==
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| | ==References== | | ==References== |
