Difference between revisions of "Team:US AFRL CarrollHS/Experiments"

Line 86: Line 86:
 
<ol>
 
<ol>
 
<li>Thaw 50 µL vial of BL21 cells on ice</li>
 
<li>Thaw 50 µL vial of BL21 cells on ice</li>
−
<li>Inoculate 2µL DNA into 50µL BL21 cells<li>
+
<li>Inoculate 2µL DNA into 50µL BL21 cells</li>
 
<li>incubate on ice for 30 minutes</li>
 
<li>incubate on ice for 30 minutes</li>
 
<li class="b">heat shock at 42℃ for 10 seconds</li>
 
<li class="b">heat shock at 42℃ for 10 seconds</li>

Revision as of 22:59, 24 October 2017


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
  3. incubate on ice for 30 minutes
  4. heat shock at 42℃ for 10 seconds
  5. incubate on ice for 5m
  6. Inoculate 250µL LB into vial
  7. incubate at 37℃ with shaking for 1.5 hours
  8. Plate 1x/9x

Methodology