Difference between revisions of "Team:US AFRL CarrollHS/Experiments"

Line 87: Line 87:
 
<li>Thaw 50 µL vial of BL21 cells on ice</li>
 
<li>Thaw 50 µL vial of BL21 cells on ice</li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells</li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells</li>
−
<li>incubate on ice for 30 minutes</li>
 
 
<ul>
 
<ul>
 +
<li>incubate on ice for 30 minutes</li>
 
<li>heat shock at 42℃ for 10 seconds</li>
 
<li>heat shock at 42℃ for 10 seconds</li>
 
<li>incubate on ice for 5m</li>
 
<li>incubate on ice for 5m</li>

Revision as of 23:03, 24 October 2017


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
    • incubate on ice for 30 minutes
    • heat shock at 42℃ for 10 seconds
    • incubate on ice for 5m
  3. Inoculate 250µL LB into vial
    • incubate at 37℃ with shaking for 1.5 hours
  4. Plate 1x/9x

Methodology