Team:US AFRL CarrollHS/Experiments


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
  4. incubate on ice for 30 minutes
  5. heat shock at 42℃ for 10 seconds
  6. incubate on ice for 5m
  7. Inoculate 250µL LB into vial
  8. incubate at 37℃ with shaking for 1.5 hours
  9. Plate 1x/9x

Methodology