Transformations
BI21 Cells:
- Thaw 50 µL vial of BL21 cells on ice
- Inoculate 2µL DNA into 50µL BL21 cells
- incubate on ice for 30 minutes
- heat shock at 42℃ for 10 seconds
- incubate on ice for 5m
- Inoculate 250µL LB into vial
- incubate at 37℃ with shaking for 1.5 hours
- Plate 1x/9x
JM109 Cells:
- Thaw vial of JM109 cells on ice
- Label 14 mL Falcon tube & plane on ice
- Inoculate 50 µL JM109 cells into Falcon tube
- Inoculate 2µL DNA into 50µL JM109 cells
- incubate on ice for 20m
- heat shock at 42℃ for 45 seconds
- Inoculate 950µL LB into vial
- incubate at 37℃ with shaking for 1.5 hours
- Plate 1x/9x
