This year we completed the 2017 Interlab to qualify for the bronze medal! Below you can find our data including our completion of the competent cell test kit, our reference points to determine background fluorescence, our standard curve, and our raw data. We collaborated alongside Cadets2Vets to determine a conclusion from each other’s data and the variation of results from team to team.
Note: We used a Molecular Device SpectraMax M5 Plate Reader to perform the Interlab
First, the students performed the Competent Cell Test Kit following the protocol provided by iGEM. The students used E. Coli DH5a as the competent cells being tested. The Competent Cell were grown on the Chloramphenicol plates and left over night. In the morning, the plates showed significant amount of growth. The growth was single pink colonies that the students believed would be easier to count. Luckily, Dr. Harbaugh had a easier method for counting using a sharpie to dot each colony and then a clicker so we wouldn’t lose your place. The students counted and the results were:
| Concentration of Plasmid DNA | Number of Single Colonies |
|---|---|
| 10 pg/μl | 254 |
| 50 pg/μl | 2105 |
| 100 pg/μl | 4121 |
Figure 1: Our reference point data providing our background fluorescence
Figure 2: Our stock fluorescence data and our standard curve
Figure 3: Raw data from each device read
Figure 4: Experimental data!
