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| </div> | | </div> |
| <div class="text"> | | <div class="text"> |
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| <h2>Week 5: June 26-30</h2> | | <h2>Week 5: June 26-30</h2> |
| <h3>Monday (6/26/17)</h3> | | <h3>Monday (6/26/17)</h3> |
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| graphics. The students will continue to add content to the wiki weekly.</p> | | graphics. The students will continue to add content to the wiki weekly.</p> |
| </div> | | </div> |
| + | |
| + | <div class="text2"> |
| + | <h2>Week 6: July 5-6</h2> |
| + | <h3>Wednesday (7/5/17)</h3> |
| + | <p>“Ring, ring, ring!” came from the laptop as the students huddled around |
| + | to receive a Skype call from the Singapore iGem team. They were greeted |
| + | by Wilbert from the Singapore team. They immediately began conversing |
| + | with Wilbert and exchanged project descriptions and ideas. The team was |
| + | very intrigued to learn about the Human Practices components of iGem |
| + | from Wilbert. After a whole hour of video conference, the team learned a lot from the |
| + | sagacious Wilbert. The conversation was very beneficial to the students as they |
| + | learned about the different components of iGem. Wilbert even offered to help them |
| + | with the wiki page! Afterwards, the students arrived back to the lab and received |
| + | presentation tips from mentors for the upcoming lab presentations the following |
| + | week.</p> |
| + | |
| + | <h3>Thursday (7/6/16)</h3> |
| + | <p>The next day, the group decided to do the InterLab in order to be |
| + | considered for medal criteria. They sifted through the kit and found many |
| + | useful parts that they could use in order to help standardize fluorescence. |
| + | After reading through the protocols and laying out a plan of attack, the |
| + | students had the wait since the lab was going under routine inspection. The students |
| + | spent the rest of the day designing more graphics and made wiki edits. In the |
| + | afternoon, the whole team got together with mentors to meet at the Air Force |
| + | Museum in Dayton, Ohio in order to take self pictures and group pictures for the |
| + | wiki.</p> |
| + | </div> |
| + | |
| + | <div class=""> |
| + | <h2>Week 7: July 10-14</h2> |
| + | |
| + | <h3>Monday (7/10/17)</h3> |
| + | <p>At 9 AM, the students went to their weekly lab meeting. Interns from their |
| + | department always have a weekly meeting where they share ideas and summer |
| + | progress. The LabPats will share their presentation next week. Afterwards, it was |
| + | finally time to begin the InterLab study for the iGem project. The students began |
| + | re suspending the wells. However, they soon found out that there were no more |
| + | plates of LB- Chloramphenicol and thus had to make more. They had to |
| + | postpone the InterLab studies and finished the day by creating a lot more plates |
| + | for future experiments.</p> |
| + | |
| + | <h3>Tuesday (7/11/17)</h3> |
| + | <p>The student researchers began the InterLab the next morning. They split up in 2 |
| + | groups while one group worked with the competent cells kit and completed Day |
| + | 1. The other group transformed the wells inside the plates provided by iGem. The |
| + | students did a great job following protocol and growing the bacteria on the agar |
| + | plates from the previous day. After they were done, the students began filling out |
| + | the necessary forms for the InterLab as well as planned out the dropdown menu and the |
| + | slideshow for the website.</p> |
| + | |
| + | <h3>Wednesday (7/12/17)</h3> |
| + | <p>The next morning, the young students attended a lecture taught by biologists |
| + | from the Purdue University. It was very interesting and higher level, and the |
| + | students were very excited to hear topics relevant to their iGem goals. |
| + | Afterwards, they returned to the lab with their mentor to examine the overnight |
| + | bacteria colonies. However, they were disappointed to learn that the competent cell test did |
| + | not work out. Instead of an expected 300+ colonies, the plates only yielded about 25 colonies. |
| + | The students spent the rest of the day redoing the protocol in hopes of achieving the needed |
| + | amount of colonies in order to proceed.</p> |
| + | |
| + | <h3>Thursday (7/13/17)</h3> |
| + | <p>Continuing the next morning, the students immediately continued the Interlab. |
| + | Today they focused on completing the OD600 and Serial Dilution part of the |
| + | protocol. The students hope to test the fluorescence of the colonies tomorrow. |
| + | Afterwards, they picked colonies and left them in the incubator to grow overnight. |
| + | Afterwards, they got back on wifi and successfully implemented a background to |
| + | the wiki. Tomorrow they will have s video conference with the U.S. Navy and U.S. Army iGem |
| + | team.</p> |
| + | |
| + | <h3>Friday (7/14/17)</h3> |
| + | <p>-“I brought doughnuts!” exclaimed Andrea as she walked in the door in the |
| + | morning. The group had planned a Skype meeting with the U.S. Navy and U.S. |
| + | Army. After getting the connection set up, the students were able to have a |
| + | myriad of conversations from topics regarding to giant Jamboree to technical |
| + | components of the wiki. After some casual conversion, the students were able to |
| + | have a better understanding of the happenings at the Jamboree and how to improve the wiki |
| + | as well as Interlab components. Next, the long part of the Interlab began. The students |
| + | tested for absorbance and fluorescence of the bacteria samples over 0,2,4, and 6 hours. |
| + | Later in the afternoon, the students did a dry run of their presentation in preparation for |
| + | Monday.</p> |
| + | </div> |
| + | |
| + | <div class="text2"> |
| + | <h2>Week 8: June 17-21</h2> |
| + | <h3>Monday (7/17/17)</h3> |
| + | <p>The student sat around nervously waiting for |
| + | their presentation to begin. In a few minutes |
| + | they would be presenting to the whole lab |
| + | about their project and goals. The students |
| + | gave a phenomenal presentation that received |
| + | much praise and applause. They were relieved to be done |
| + | and went back to work on the wiki. Everything in the lab is |
| + | up to date so the young researchers dedicated the rest of |
| + | the day for wiki organization and miscellaneous tasks.</p> |
| + | |
| + | <h3>Tuesday (7/18/17)</h3> |
| + | <p>Mentor Mike was finally back from vacation and the students were very excited |
| + | to see him and update him on current progress. They discussed the plan moving |
| + | forward and the layout of the rest of the |
| + | summer. After half an hour, the students had a |
| + | general idea of the "end goal". They discussed |
| + | the DNA sequencing that had just come in and were like |
| + | described. Afterwards the students continued working on |
| + | collaboration and organizing wiki pages.</p> |
| + | |
| + | <h3>Thursday (7/20/17)</h3> |
| + | <p>The day started at UES and the students |
| + | began to look up literature that would help |
| + | them with the sensing part of the project goal. |
| + | They were able to find lots of useful |
| + | information referring to the two ETEC toxins, a |
| + | heat labile and heat stabile. Over the course of a couple |
| + | hours, the students were able to find and compose a list of |
| + | the possible ways to detect ETEC. In the afternoon, the |
| + | students shared the ideas with their mentor, Mike. After |
| + | lunch, the students discussed what they had gathered from |
| + | the past 3 days of researching literature. After exchanging |
| + | ideas and articles, the students and Dr. Goodson realized that being able to sense a quorum |
| + | sensing molecule autoinducer-2 (AI-2) might be the way to go. They discussed at length the |
| + | necessary components needed to be able to put the promoter into the existing plasmid so that |
| + | it could detect AI-2. After a bit of research, they decided to go with E. coli DH5a which fit |
| + | perfectly as it does not create AI-2 on its own. The students were relieved that they had taken a |
| + | massive step forward in their “sensing” |
| + | component and hope to achieve the end goal |
| + | soon.</p> |
| + | |
| + | <h3>Friday (7/21/17)</h3> |
| + | <p>The students finally began the lab |
| + | work today for "sensing". The |
| + | students worked on resuspending |
| + | part K18 in Well 3 and followed the |
| + | protocol to grow them up on plates. |
| + | They also transformed the GFPa1 plasmid into |
| + | DH5a E. coli cells. The plates were then left |
| + | outside overnight to grow. Meanwhile, the |
| + | young researchers were able to analyze the |
| + | plasmid and look at all the restriction sites to |
| + | find the ideal sites to cut the plasmid. Under the |
| + | guidance of Dr. Goodson, the students picked |
| + | three restriction sites and an order was put in to |
| + | order the primers. Afterwards, the RH lab held |
| + | their weekly lab meeting with the RX lab and |
| + | shared the exciting news of their new discovery.</p> |
| + | </div> |
| + | |
| + | <div class="text"> |
| + | <h2></h2> |
| + | <h3>Monday (7/24/17)</h3> |
| + | <p></p> |
| + | |
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| + | <p></p> |
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| + | <p></p> |
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| + | <h3></h3> |
| + | <p></p> |
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| + | <h3></h3> |
| + | <p></p> |
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| + | <h3></h3> |
| + | <p></p> |
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