|
|
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| | | |
| <h3>Wednesday (06/07/17)</h3> | | <h3>Wednesday (06/07/17)</h3> |
− | <p><b>Sense/Respond:</b>On the first day in the lab the members made LB broth, (formula in | + | <p><b>Sense/Respond: </b>On the first day in the lab the members made LB broth, (formula in |
| protocol tab). Although a simple procedure, this was the first hands-on | | protocol tab). Although a simple procedure, this was the first hands-on |
| experience for the students in the lab. They then did a plasma | | experience for the students in the lab. They then did a plasma |
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| | | |
| <h3>Thursday (06/08/17)</h3> | | <h3>Thursday (06/08/17)</h3> |
− | <p><b>Sense/Respond:</b>When they entered the lab on the second day the plates showed growth | + | <p><b>Sense/Respond: </b>When they entered the lab on the second day the plates showed growth |
| with distinctions of individual | | with distinctions of individual |
| colonies. Mentor Mike Goodson | | colonies. Mentor Mike Goodson |
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| | | |
| <h3>Friday (06/09/17)</h3> | | <h3>Friday (06/09/17)</h3> |
− | <p><b>Sense/Respond:</b>For the last day of the week, the students’ goal was to confirm the E. Coli | + | <p><b>Sense/Respond: </b>For the last day of the week, the students’ goal was to confirm the E. Coli |
| had absorbed the plasmid and then perform a MiniPrep Kit to take the | | had absorbed the plasmid and then perform a MiniPrep Kit to take the |
| plasmid out of the bacteria. To accomplish this, they first learned how to | | plasmid out of the bacteria. To accomplish this, they first learned how to |
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| <h2>Week 3: June 12-16</h2> | | <h2>Week 3: June 12-16</h2> |
| <h3>Monday (06/12/17)</h3> | | <h3>Monday (06/12/17)</h3> |
− | <p><b>Sense/Respond:</b>The students went to a group meeting within their labs. Students | + | <p><b>Sense/Respond: </b>The students went to a group meeting within their labs. Students |
| streaked bacteria onto agar plates and put back into the incubator. The | | streaked bacteria onto agar plates and put back into the incubator. The |
| rest of the day was spent collaborating on website design and graphic | | rest of the day was spent collaborating on website design and graphic |
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| | | |
| <h3>Tuesday (06/13/17)</h3> | | <h3>Tuesday (06/13/17)</h3> |
− | <p><b>Sense/Respond:</b>The students removed the agar plates with bacteria from the incubator. | + | <p><b>Sense/Respond: </b>The students removed the agar plates with bacteria from the incubator. |
| Two tubes were filled with LB broth, one labeled as a blank and one | | Two tubes were filled with LB broth, one labeled as a blank and one |
| containing the bacteria. Using a sterile toothpick, a single colony was picked | | containing the bacteria. Using a sterile toothpick, a single colony was picked |
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| | | |
| <h3>Wednesday (06/14/17)</h3> | | <h3>Wednesday (06/14/17)</h3> |
− | <p><b>Sense/Respond:</b>The students retrieved the test tubes placed in the incubator the day before | + | <p><b>Sense/Respond: </b>The students retrieved the test tubes placed in the incubator the day before |
| and analytically checked the optical density and the fluorescence of the | | and analytically checked the optical density and the fluorescence of the |
| colonies. Immediately afterwards, a visual check was performed as well, | | colonies. Immediately afterwards, a visual check was performed as well, |
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| | | |
| <h3>Thursday (06/15/17)</h3> | | <h3>Thursday (06/15/17)</h3> |
− | <p><b>Sense/Respond:</b>Time to go back to work bright and early. A little after the crack of dawn, | + | <p><b>Sense/Respond: </b>Time to go back to work bright and early. A little after the crack of dawn, |
| the engaged students immediately went to work preparing a digest. The | | the engaged students immediately went to work preparing a digest. The |
| scholars made a 15 uL digest along with a Master Mix. Interactive steps | | scholars made a 15 uL digest along with a Master Mix. Interactive steps |
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| | | |
| <h3>Friday (06/16/17)</h3> | | <h3>Friday (06/16/17)</h3> |
− | <p><b>Sense/Respond:</b>Immediately the next day, the eager students were right back at work. The | + | <p><b>Sense/Respond: </b>Immediately the next day, the eager students were right back at work. The |
| previous day's failure had fired them up and they were eager to do | | previous day's failure had fired them up and they were eager to do |
| everything carefully and quickly. The day began with a student-led mini-prep | | everything carefully and quickly. The day began with a student-led mini-prep |
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| <h2>Week 4: June 19-23</h2> | | <h2>Week 4: June 19-23</h2> |
| <h3>Monday (06/19/17)</h3> | | <h3>Monday (06/19/17)</h3> |
− | <p>After the weekend, the students met in the lab and initiated a second | + | <p><b>Sense/Respond: </b>After the weekend, the students met in the lab and initiated a second |
| digest. They were determined to get everything measured correctly and | | digest. They were determined to get everything measured correctly and |
| leave minimal room for mistakes. This time around, the students were | | leave minimal room for mistakes. This time around, the students were |
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| | | |
| <h3>Tuesday (06/20/17)</h3> | | <h3>Tuesday (06/20/17)</h3> |
− | <p>The students quickly resumed where they had left off to begin their gel | + | <p><b>Sense/Respond: </b>The students quickly resumed where they had left off to begin their gel |
| extraction. Two different protocols were used to see which one would | | extraction. Two different protocols were used to see which one would |
| achieve a higher concentration. The teams were divided into Tina and Annie | | achieve a higher concentration. The teams were divided into Tina and Annie |
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| | | |
| <h3>Wednesday (06/21/17)</h3> | | <h3>Wednesday (06/21/17)</h3> |
− | <p>Today the students began their second Polymerase Chain Reaction (PCR). | + | <p><b>Sense/Respond: </b>Today the students began their second Polymerase Chain Reaction (PCR). |
| Under the watchful eye of Dr. Harbaugh, the students independently finished | | Under the watchful eye of Dr. Harbaugh, the students independently finished |
| the PCR and loaded it into the thermocycler. Another small gel was prepared | | the PCR and loaded it into the thermocycler. Another small gel was prepared |
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| | | |
| <h3>Thursday (6/22/17)</h3> | | <h3>Thursday (6/22/17)</h3> |
− | <p>The day began with another PCR. Today, the students were ready to | + | <p><b>Sense/Respond: </b>The day began with another PCR. Today, the students were ready to |
| completely do the PCR by themselves with little guidance from the mentors. | | completely do the PCR by themselves with little guidance from the mentors. |
| The standard protocol was followed and the PCR and ran another cycle in | | The standard protocol was followed and the PCR and ran another cycle in |
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| | | |
| <h3>Friday (6/23/17)</h3> | | <h3>Friday (6/23/17)</h3> |
− | <p>The day began bright and early with two PCR digests. This time a PCR | + | <p><b>Sense/Respond: </b>The day began bright and early with two PCR digests. This time a PCR |
| Vector Digest was conducted on the same from yesterday and a PCR GFP | | Vector Digest was conducted on the same from yesterday and a PCR GFP |
| digest was done on the sample from Wednesday (6/21/17). After following | | digest was done on the sample from Wednesday (6/21/17). After following |
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| <h2>Week 5: June 26-30</h2> | | <h2>Week 5: June 26-30</h2> |
| <h3>Monday (6/26/17)</h3> | | <h3>Monday (6/26/17)</h3> |
− | <p>The students began another week of hard work. They made a gel to run | + | <p><b>Sense/Respond: </b>The students began another week of hard work. They made a gel to run |
| their previous Plasmid Digest. Next, they looked at the gel under the blue | | their previous Plasmid Digest. Next, they looked at the gel under the blue |
| light and found the band they were looking for at 3.2k base pairs. Next they | | light and found the band they were looking for at 3.2k base pairs. Next they |
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| | | |
| <h3>Tuesday (6/27/17)</h3> | | <h3>Tuesday (6/27/17)</h3> |
− | <p>Today was the student's first ligation. The goal was to put the sticky ends | + | <p><b>Sense/Respond: </b>Today was the student's first ligation. The goal was to put the sticky ends |
| back together to form a complete plasmid. A lot of math was done to | | back together to form a complete plasmid. A lot of math was done to |
| determine the exact ratios of sfGFP and PCR vector plasmid. Luckily, Dr. | | determine the exact ratios of sfGFP and PCR vector plasmid. Luckily, Dr. |
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| | | |
| <h3>Wednesday (6/28/17)</h3> | | <h3>Wednesday (6/28/17)</h3> |
− | <p>The students began the day with a new PCR. After examining the plates from | + | <p><b>Sense/Respond: </b>The students began the day with a new PCR. After examining the plates from |
| yesterday, it was determined that there indeed was green fluorescence on the | | yesterday, it was determined that there indeed was green fluorescence on the |
| plates. However, a PCR was needed with a gel to determine whether or not it was | | plates. However, a PCR was needed with a gel to determine whether or not it was |
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| | | |
| <h3>Thursday (6/29/17)</h3> | | <h3>Thursday (6/29/17)</h3> |
− | <p>Today the students began to mini-prep the samples from the previous day. | + | <p><b>Sense/Respond: </b>Today the students began to mini-prep the samples from the previous day. |
| Right from the start, the students noticed fluorescent green coming from | | Right from the start, the students noticed fluorescent green coming from |
| one of the tubes. This would foreshadow what they would see later in the | | one of the tubes. This would foreshadow what they would see later in the |
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| | | |
| <h3>Friday (6/30/17)</h3> | | <h3>Friday (6/30/17)</h3> |
− | <p>Since all the lab work is currently done until the DNA sample is sent to Ohio State | + | <p><b>Sense/Respond: </b>Since all the lab work is currently done until the DNA sample is sent to Ohio State |
| University for DNA sequencing, the students had the whole day to make website | | University for DNA sequencing, the students had the whole day to make website |
| improvements and wiki renovations. New logos were also created as well as | | improvements and wiki renovations. New logos were also created as well as |
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| sagacious Wilbert. The conversation was very beneficial to the students as they | | sagacious Wilbert. The conversation was very beneficial to the students as they |
| learned about the different components of iGem. Wilbert even offered to help them | | learned about the different components of iGem. Wilbert even offered to help them |
− | with the wiki page! Afterwards, the students arrived back to the lab and received | + | with the wiki page!</p> |
| + | |
| + | <p><b>Sense/Respond: </b>Afterwards, the students arrived back to the lab and received |
| presentation tips from mentors for the upcoming lab presentations the following | | presentation tips from mentors for the upcoming lab presentations the following |
| week.</p> | | week.</p> |
| | | |
| <h3>Thursday (7/6/16)</h3> | | <h3>Thursday (7/6/16)</h3> |
− | <p>The next day, the group decided to do the InterLab in order to be | + | <p><b>Sense/Respond: </b>The next day, the group decided to do the InterLab in order to be |
| considered for medal criteria. They sifted through the kit and found many | | considered for medal criteria. They sifted through the kit and found many |
| useful parts that they could use in order to help standardize fluorescence. | | useful parts that they could use in order to help standardize fluorescence. |
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| | | |
| <h3>Monday (7/10/17)</h3> | | <h3>Monday (7/10/17)</h3> |
− | <p>At 9 AM, the students went to their weekly lab meeting. Interns from their | + | <p><b>Sense/Respond: </b>At 9 AM, the students went to their weekly lab meeting. Interns from their |
| department always have a weekly meeting where they share ideas and summer | | department always have a weekly meeting where they share ideas and summer |
| progress. The LabPats will share their presentation next week. Afterwards, it was | | progress. The LabPats will share their presentation next week. Afterwards, it was |
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| | | |
| <h3>Tuesday (7/11/17)</h3> | | <h3>Tuesday (7/11/17)</h3> |
− | <p>The student researchers began the InterLab the next morning. They split up in 2 | + | <p><b>Sense/Respond: </b>The student researchers began the InterLab the next morning. They split up in 2 |
| groups while one group worked with the competent cells kit and completed Day | | groups while one group worked with the competent cells kit and completed Day |
| 1. The other group transformed the wells inside the plates provided by iGem. The | | 1. The other group transformed the wells inside the plates provided by iGem. The |
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| | | |
| <h3>Wednesday (7/12/17)</h3> | | <h3>Wednesday (7/12/17)</h3> |
− | <p>The next morning, the young students attended a lecture taught by biologists | + | <p><b>Sense/Respond: </b>The next morning, the young students attended a lecture taught by biologists |
| from the Purdue University. It was very interesting and higher level, and the | | from the Purdue University. It was very interesting and higher level, and the |
| students were very excited to hear topics relevant to their iGem goals. | | students were very excited to hear topics relevant to their iGem goals. |
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| | | |
| <h3>Thursday (7/13/17)</h3> | | <h3>Thursday (7/13/17)</h3> |
− | <p>Continuing the next morning, the students immediately continued the Interlab. | + | <p><b>Sense/Respond: </b>Continuing the next morning, the students immediately continued the Interlab. |
| Today they focused on completing the OD600 and Serial Dilution part of the | | Today they focused on completing the OD600 and Serial Dilution part of the |
| protocol. The students hope to test the fluorescence of the colonies tomorrow. | | protocol. The students hope to test the fluorescence of the colonies tomorrow. |
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| components of the wiki. After some casual conversion, the students were able to | | components of the wiki. After some casual conversion, the students were able to |
| have a better understanding of the happenings at the Jamboree and how to improve the wiki | | have a better understanding of the happenings at the Jamboree and how to improve the wiki |
− | as well as Interlab components. Next, the long part of the Interlab began. The students | + | as well as Interlab components. </p> |
| + | <p><b>Sense/Respond: </b> |
| + | Next, the long part of the Interlab began. The students |
| tested for absorbance and fluorescence of the bacteria samples over 0,2,4, and 6 hours. | | tested for absorbance and fluorescence of the bacteria samples over 0,2,4, and 6 hours. |
| Later in the afternoon, the students did a dry run of their presentation in preparation for | | Later in the afternoon, the students did a dry run of their presentation in preparation for |
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| <h2>Week 8: June 17-21</h2> | | <h2>Week 8: June 17-21</h2> |
| <h3>Monday (7/17/17)</h3> | | <h3>Monday (7/17/17)</h3> |
− | <p>The student sat around nervously waiting for | + | <p><b>Sense/Respond: </b>The student sat around nervously waiting for |
| their presentation to begin. In a few minutes | | their presentation to begin. In a few minutes |
| they would be presenting to the whole lab | | they would be presenting to the whole lab |
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| | | |
| <h3>Tuesday (7/18/17)</h3> | | <h3>Tuesday (7/18/17)</h3> |
− | <p>Mentor Mike was finally back from vacation and the students were very excited | + | <p><b>Sense/Respond: </b>Mentor Mike was finally back from vacation and the students were very excited |
| to see him and update him on current progress. They discussed the plan moving | | to see him and update him on current progress. They discussed the plan moving |
| forward and the layout of the rest of the | | forward and the layout of the rest of the |
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| | | |
| <h3>Thursday (7/20/17)</h3> | | <h3>Thursday (7/20/17)</h3> |
− | <p>The day started at UES and the students | + | <p><b>Sense/Respond: </b>The day started at UES and the students |
| began to look up literature that would help | | began to look up literature that would help |
| them with the sensing part of the project goal. | | them with the sensing part of the project goal. |
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| | | |
| <h3>Friday (7/21/17)</h3> | | <h3>Friday (7/21/17)</h3> |
− | <p>The students finally began the lab | + | <p><b>Sense/Respond: </b>The students finally began the lab |
| work today for "sensing". The | | work today for "sensing". The |
| students worked on resuspending | | students worked on resuspending |
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| <h2>Week 9: July 24-28</h2> | | <h2>Week 9: July 24-28</h2> |
| <h3>Monday (7/24/17)</h3> | | <h3>Monday (7/24/17)</h3> |
− | <p>The day was a bit more relaxed for the Monday morning. The weekly lab | + | <p><b>Sense/Respond: </b>The day was a bit more relaxed for the Monday morning. The weekly lab |
| meeting was scheduled and the students were able to learn a lot about other | | meeting was scheduled and the students were able to learn a lot about other |
| researchers and their current studies. In addition, they learned how to present to | | researchers and their current studies. In addition, they learned how to present to |
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| | | |
| <h3>Tuesday (7/25/17)</h3> | | <h3>Tuesday (7/25/17)</h3> |
− | <p>The adventures continue the next morning as the team went back into the lab to | + | <p><b>Sense/Respond: </b>The adventures continue the next morning as the team went back into the lab to |
| look at the overnight cultures. Tina and Hayley were extremely pleased to find | | look at the overnight cultures. Tina and Hayley were extremely pleased to find |
| their cultures had grown perfectly and were ready to do the mini-prep and nano | | their cultures had grown perfectly and were ready to do the mini-prep and nano |
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| <p>Another meeting was held to discuss the presentation and viability of the | | <p>Another meeting was held to discuss the presentation and viability of the |
| Michigan State Trip. Students from both labs went over their combined | | Michigan State Trip. Students from both labs went over their combined |
− | presentation and rehearsed thoroughly. Later in the lab. Hayley and Tina prepared | + | presentation and rehearsed thoroughly.</p> |
| + | <p><b>Sense/Respond: </b> Hayley and Tina prepared |
| cultures of GFPa1 and sfGFP from the plates. Dallas transformed the iGem | | cultures of GFPa1 and sfGFP from the plates. Dallas transformed the iGem |
| cultures but sadly failed. Dr. Goodson recommended that they regrow the plates | | cultures but sadly failed. Dr. Goodson recommended that they regrow the plates |
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| attend and give them support. The presentation | | attend and give them support. The presentation |
| went very well and the RX lab members received a | | went very well and the RX lab members received a |
− | stellar ovation. Later in the afternoon, the RH lab | + | stellar ovation.</p> |
| + | <p><b>Sense/Respond: </b>Later in the afternoon, the RH lab |
| would be preparing a mini-prep for the Well 3, 18K | | would be preparing a mini-prep for the Well 3, 18K |
| competent cells provided by iGem. The students | | competent cells provided by iGem. The students |
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| <h2>Week 10: July 31-August 4</h2> | | <h2>Week 10: July 31-August 4</h2> |
| <h3>Monday (7/31/17)</h3> | | <h3>Monday (7/31/17)</h3> |
− | <p>The morning started with the students performing a digest over the mini prep | + | <p><b>Sense/Respond: </b>The morning started with the students performing a digest over the mini prep |
| from several days pervious. Two digests were preformed using different sets of | | from several days pervious. Two digests were preformed using different sets of |
| cut enzymes. Digest 1 was using the SpnI-HF and BmrI, while Digest 2 used the | | cut enzymes. Digest 1 was using the SpnI-HF and BmrI, while Digest 2 used the |
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| | | |
| <h3>Tuesday (8/1/17)</h3> | | <h3>Tuesday (8/1/17)</h3> |
− | <p>The students set up another run of their GFP Project. The students used a | + | <p><b>Sense/Respond: </b>The students set up another run of their GFP Project. The students used a |
| Kinetics reading of the Plate Reader. The kinetics were taken over 24 hours, every | | Kinetics reading of the Plate Reader. The kinetics were taken over 24 hours, every |
| 20 minutes. The students then worked on the wiki, trying to create a template.</p> | | 20 minutes. The students then worked on the wiki, trying to create a template.</p> |
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| | | |
| <h3>Thursday (8/3/17)</h3> | | <h3>Thursday (8/3/17)</h3> |
− | <p>Today the students got back to work on the sense plasmid. The students | + | <p><b>Sense/Respond: </b>Today the students got back to work on the sense plasmid. The students |
| performed a PCR, because finally their primers came in. The PCR product was | | performed a PCR, because finally their primers came in. The PCR product was |
| ran on the gel and the students got the results they were hoping for. Next, they | | ran on the gel and the students got the results they were hoping for. Next, they |
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| present later. They left in high spirits to return to the lab and begin the | | present later. They left in high spirits to return to the lab and begin the |
| purification, ligation, and transformation of the plasmid for the “sense” | | purification, ligation, and transformation of the plasmid for the “sense” |
− | component of the project. The purification was then nanodroped and the students were | + | component of the project.</p> |
| + | <p><b>Sense/Respond: </b> |
| + | The purification was then nanodroped and the students were |
| pleased with the results. The students then performed a ligation on the plasmid backbone | | pleased with the results. The students then performed a ligation on the plasmid backbone |
| from the iGEM part and the sfGFP. The ligation seemed to go as planned and the students | | from the iGEM part and the sfGFP. The ligation seemed to go as planned and the students |
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| <h2>Week 11: August 7-11</h2> | | <h2>Week 11: August 7-11</h2> |
| <h3>Monday (8/7/17)</h3> | | <h3>Monday (8/7/17)</h3> |
− | <p>Unfortunately the students were disheartened to learn that none of the plates | + | <p><b>Sense/Respond: </b>Unfortunately the students were disheartened to learn that none of the plates |
| grew colonies. The students were at first shocked, but immediately started | | grew colonies. The students were at first shocked, but immediately started |
| tracing back to the different protocols and plasmids from the past few days. | | tracing back to the different protocols and plasmids from the past few days. |
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| | | |
| <h3>Tuesday (8/8/17)</h3> | | <h3>Tuesday (8/8/17)</h3> |
− | <p>Today the LabPats were finally able to transform the plasmid from the ligation | + | <p><b>Sense/Respond: </b>Today the LabPats were finally able to transform the plasmid from the ligation |
| yesterday. They followed the standard protocol and transformed the cells into E. | | yesterday. They followed the standard protocol and transformed the cells into E. |
| coli DH5a again. After that, the cells were ready to be plated like previously. This | | coli DH5a again. After that, the cells were ready to be plated like previously. This |
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| | | |
| <h3>Wednesday (8/9/17)</h3> | | <h3>Wednesday (8/9/17)</h3> |
− | <p>The students eagerly went into the lab to check the plates from the | + | <p><b>Sense/Respond: </b>The students eagerly went into the lab to check the plates from the |
| transformation. To the students dismay all the plates contained growth even the | | transformation. To the students dismay all the plates contained growth even the |
| blanks. The concerned students went to ask Mentor Mike, who help the student | | blanks. The concerned students went to ask Mentor Mike, who help the student |
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| | | |
| <h3>Thursday (8/10/17)</h3> | | <h3>Thursday (8/10/17)</h3> |
− | <p>Bright and early the students got their overnights out of the incubator. They then | + | <p><b>Sense/Respond: </b>Bright and early the students got their overnights out of the incubator. They then |
| made glycerol stocks and performed a mini prep, so the bacteria could be sent | | made glycerol stocks and performed a mini prep, so the bacteria could be sent |
| for sequencing. In the afternoon, the students got to view a set of poster | | for sequencing. In the afternoon, the students got to view a set of poster |
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| | | |
| <h3>Friday (8/11/17)</h3> | | <h3>Friday (8/11/17)</h3> |
− | <p>Today was a sad day in the lab, as the summer was ending and the students | + | <p><b>Sense/Respond: </b>Today was a sad day in the lab, as the summer was ending and the students |
| had to return to school. The team spent the morning taking their mentors to | | had to return to school. The team spent the morning taking their mentors to |
| breakfast, as a thank you for all they had done for us. The rest of the day was | | breakfast, as a thank you for all they had done for us. The rest of the day was |