Difference between revisions of "Team:US AFRL CarrollHS/Results"

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<p>At the conclusion of our project we had created three working plasmids, all of which were sent to the parts registry.</p>
 
<p>At the conclusion of our project we had created three working plasmids, all of which were sent to the parts registry.</p>
 
<p>Part BBa_K2522000 is a plasmid developed using the csgA gene within E. coli’s genome, and a double cellulose binding domain taken from the DNA distribution kit. Sequencing was performed to confirm our DNA matched our theoretical design, yet no tests have been performed to develop a working construct (Ability to bind to cellulose, ect.)</p>
 
<p>Part BBa_K2522000 is a plasmid developed using the csgA gene within E. coli’s genome, and a double cellulose binding domain taken from the DNA distribution kit. Sequencing was performed to confirm our DNA matched our theoretical design, yet no tests have been performed to develop a working construct (Ability to bind to cellulose, ect.)</p>
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<li> Added protocols to the U of Michigan – Software’s website project: Protocat. </li>
 
<li> Added protocols to the U of Michigan – Software’s website project: Protocat. </li>
 
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Revision as of 20:11, 30 October 2017


Results

At the conclusion of our project we had created three working plasmids, all of which were sent to the parts registry.

Part BBa_K2522000 is a plasmid developed using the csgA gene within E. coli’s genome, and a double cellulose binding domain taken from the DNA distribution kit. Sequencing was performed to confirm our DNA matched our theoretical design, yet no tests have been performed to develop a working construct (Ability to bind to cellulose, ect.)

Part BBa_K2522001 is a working construct developed to produce Super Folder Green Fluorescent Protein in the presence of the quorum sensing molecule 3OC12. The DNA is confirmed to work as theoretically planned thrpugh both sequencing and experiment. (INSERT 96 WELL PIC HERE0

Part BBa_K2522002 is a plasmid designed to produce sfGFP in the presence of the quorum sensing molecule Auto Inducer 2 (AI-2). While confirmed to match our theoretical design via sequencing, due to time constraints only one experiment was performed. The plasmid was grown up overnight along with supernatant from a E.coli Nissle 1917 sample (which in theory should contain AI-2); however, the test was in conclusive due to contamination (bacterial growth in pure LB broth used as our blank)

We also characterized our two choices for green fluorescent protein: GFPa1 and sfGFP. Below are our results indicating that sfGFP is best for our project due to the short time taken to fluoresce (~ 4 hours to a visible level) and it’s use in a diagnostic environment that our plasmid would be used for.


In conclusion we:

  • Characterized and submitted 3 parts to the registry
  • Compared and characterized two of our GFPs
  • Successfully Completed Interlab
  • Created presentation accessible to grade schoolers and a brochure aimed towards the general public.
  • Gained a better understanding of the interlab project through collaboration with Cadets2Vets
  • Received modeling results from the National University of Singapore.
  • Added protocols to the U of Michigan – Software’s website project: Protocat.