Difference between revisions of "Team:US AFRL CarrollHS/Experiments"

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     <h1>Experiment</h1>
 
     <h1>Experiment</h1>
 
     <p><b> </p></b>
 
     <p><b> </p></b>
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    </div>
 
 
   </div>
 
   </div>
 
</div>
 
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<li>Inoculate 2µL DNA into 50µL BL21 cells<li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells<li>
 
<li>incubate on ice for 30 minutes</li>
 
<li>incubate on ice for 30 minutes</li>
−
<li.b>heat shock at 42℃ for 10 seconds</li>
+
<li class="b">heat shock at 42℃ for 10 seconds</li>
−
<li.b>incubate on ice for 5m</li>
+
<li class="b">incubate on ice for 5m</li>
 
<li>Inoculate 250µL LB into vial</li>
 
<li>Inoculate 250µL LB into vial</li>
−
<li.b>incubate at 37℃ with shaking for 1.5 hours</li>
+
<li class="b">incubate at 37℃ with shaking for 1.5 hours</li>
 
<li>Plate 1x/9x</li>
 
<li>Plate 1x/9x</li>
 
</ol>
 
</ol>

Revision as of 22:57, 24 October 2017


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
  4. incubate on ice for 30 minutes
  5. heat shock at 42℃ for 10 seconds
  6. incubate on ice for 5m
  7. Inoculate 250µL LB into vial
  8. incubate at 37℃ with shaking for 1.5 hours
  9. Plate 1x/9x

Methodology