Difference between revisions of "Team:Tongji China/Results"

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{{Tongji_China}}
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<!-- This document, originally from getmdl.io, was modified -->
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<html>
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<!--
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  Material Design Lite
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  Copyright 2015 Google Inc. All rights reserved.
  
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<div class="column full_size" >
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  Licensed under the Apache License, Version 2.0 (the "License");
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  you may not use this file except in compliance with the License.
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  You may obtain a copy of the License at
  
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<h1>Results</h1>
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      https://www.apache.org/licenses/LICENSE-2.0
  
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<p>Here you can describe the results of your project and your future plans. </p>
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  Unless required by applicable law or agreed to in writing, software
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  distributed under the License is distributed on an "AS IS" BASIS,
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  WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
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  See the License for the specific language governing permissions and
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  limitations under the License
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-->
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{{Tongji_China/Header}}
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<html lang="en">
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  <head>
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    <meta charset="utf-8">
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    <meta http-equiv="X-UA-Compatible" content="IE=edge">
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    <meta name="description" content="2017 Tongji iGEM team wiki">
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    <meta name="viewport" content="width=device-width, initial-scale=1.0, minimum-scale=1.0">
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    <title>Tongji iGEM - InterLab</title>
  
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<h5>What should this page contain?</h5>
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    <script src="https://2017.igem.org/Template:Tongji_China/Javascript?action=raw&ctype=text/javascript"></script>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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</ul>
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<h5>You should also describe what your results mean: </h5>
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    <!-- Page styles -->
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    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Tongji_China/CSS?action=raw&ctype=text/css">
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    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Tongji_China/CSS_2?action=raw&ctype=text/css">
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    <link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Tongji_China/CSS_3?action=raw&ctype=text/css">
  
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<ul>
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    <style>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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    #view-source {
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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      position: fixed;
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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      display: block;
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</ul>
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      right: 0;
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      bottom: 0;
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      margin-right: 40px;
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      margin-bottom: 40px;
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      z-index: 900;
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    }
  
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</div>
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    .demo-card-wide.mdl-card {
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      margin: auto;
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      margin-top: 20px;
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      align-items: center;
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      width: 70%;
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      border-radius:6px
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    }
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    .demo-card-wide > .mdl-card__title {
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      color: #757575;
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      align-self: center;
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      /*height: 176px;*/
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    }
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    .demo-card-wide > .mdl-card__menu {
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      color: #757575;
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    }
  
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<div class="clear"></div>
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    .mdl-card__title-text {
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      padding-top: 20px;
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    }
  
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<div class="column half_size" >
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    .mdl-card__supporting-text {
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    }
  
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    @media only screen and (max-device-width: 1200px) {
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      .demo-card-wide.mdl-card {
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        width: 80%;
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        max-width: 1500px
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      }
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  }
  
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<h5> Project Achievements </h5>
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    @media only screen and (max-device-width: 900px) {
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      .demo-card-wide.mdl-card {
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        width: 90%;
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      }
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  }
  
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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    @media only screen and (max-device-width: 600px) {
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      .demo-card-wide.mdl-card {
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        width: 95%;
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      }
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  }
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    </style>
  
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<ul>
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    <script type="text/javascript">
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<li>A list of linked bullet points of the successful results during your project</li>
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    // Try to remove weird iGEM side menu and resize the remaining things
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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    function closeMenuDiv(){
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</ul>
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      document.getElementById("sideMenu").style.display = "none";
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      bars_box_active = false;
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      document.getElementById('content').setAttribute("style","display:block;width:100%");
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      document.getElementById('content').style.width='100%';
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    }
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    window.setTimeout(closeMenuDiv,50);
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    window.setTimeout(closeMenuDiv,100);
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    window.setTimeout(closeMenuDiv,500);
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    window.setTimeout(closeMenuDiv,1000);
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    window.setTimeout(closeMenuDiv,5000);
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    window.setTimeout(closeMenuDiv,10000);
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    </script>
  
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</div>
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  </head>
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  <body>
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    <div class="mdl-layout mdl-js-layout mdl-layout--fixed-header" class="main view" style="background:#f9f9f9">
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      <!-- Header -->
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      <div class="android-header mdl-layout__header mdl-layout__header--waterfall">
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        <div class="mdl-layout__header-row">
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          <span class="android-title mdl-layout-title">
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            <div class="logo-font">Tongji iGEM</div>
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          </span>
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          <!-- Add spacer, to align navigation to the right in desktop -->
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          <div class="android-header-spacer mdl-layout-spacer"></div>
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          <!-- Navigation -->
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          <div class="android-navigation-container">
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            <nav class="android-navigation mdl-navigation">
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              <!-- Links in the top right -->
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              <a class="mdl-navigation__link mdl-typography--text-uppercase" href="https://2017.igem.org/Team:Tongji_China">Home</a>
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              <a class="mdl-navigation__link mdl-typography--text-uppercase" href="https://2017.igem.org/Team:Tongji_China/Description">Project</a>
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              <a class="mdl-navigation__link mdl-typography--text-uppercase" href="https://2017.igem.org/Team:Tongji_China/Team">Team</a>
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              <a class="mdl-navigation__link mdl-typography--text-uppercase" href="https://2017.igem.org/Team:Tongji_China/Results">Results</a>
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            </nav>
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          </div>
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          <span class="android-mobile-title mdl-layout-title">
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            <div class="logo-font">TongJi iGEM</div>
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          </span>
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        </div>
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      </div>
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      <!-- Drawer -->
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      <div class="android-drawer mdl-layout__drawer">
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        <nav class="mdl-navigation">
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          <!-- <span class="mdl-navigation__link" href="">Title</span> -->
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China">HOME</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Team">Team</a>
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          <div class="android-drawer-separator"></div>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Description">Description</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Design">Design</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Experiments">Experiment</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Model">Model</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Demonstrate">Demonstrate</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Results">Results</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Notebook">Notebook</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Improve">Improve</a>
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          <div class="android-drawer-separator"></div>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Parts">Parts</a>
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          <div class="android-drawer-separator"></div>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Collaborations">Collaborations</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Human_Practices">Human Practices</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/HP/Silver">HP Silver</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/HP/Gold_Integrated">HP Gold</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Engagement">Education & Public Engagement</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/InterLab">InterLab</a>
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          <!-- <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Contribution">Contribution</a> -->
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Safety">Safety</a>
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          <a class="mdl-navigation__link" href="https://2017.igem.org/Team:Tongji_China/Attributions">Attributions</a>
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          <div class="android-drawer-separator"></div>
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          <div class="android-drawer-separator"></div>
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        </nav>
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      </div>
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      <a name="top"></a>
  
 +
      <!-- HERE STARTS THE PAGE -->
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      <div class="android-content mdl-layout__content">
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        <a name="top"></a>
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        <!-- Title and Subtitle -->
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        <div class="mdl-typography--text-center" style="margin-bottom:20%">
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          <div class="logo-font android-slogan" style="color:#388E3C;">Results</div>
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          <div class="logo-font android-sub-slogan" style="color:#757575;">
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            The outcomes of our experiments.<br>
 +
            <i class="material-icons">expand_more</i>
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          </div>
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        </div>
  
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<div class="column half_size" >
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        <!-- Plasmid Construction -->
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        <div class="demo-card-wide mdl-card mdl-shadow--2dp">
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          <div class="mdl-card__title" style="text-align:center">
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            <h4 class="mdl-card__title-text" style="font-size: 250%; color:#5a5a5a">Plasmid Construction</h4>
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          </div>
 +
          <div class="mdl-card__supporting-text" style="font-size: 115%">
 +
            We constructed three plasmid successfully: pUAST-pleP-Gal4, pUAST-pleP-Gal80ts and pUAST-UAS-TH.<br>
 +
            This is our digest verification map:<br><br>
  
−
<h5>Inspiration</h5>
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            <div style="width:100%">
−
<p>See how other teams presented their results.</p>
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              <div class="demo-card-wide mdl-card mdl-shadow--2dp" style="width:30%; float:left; margin:0%; margin-right:5%">
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<ul>
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                <img src="https://static.igem.org/mediawiki/2017/e/e3/2017tongji_wiki_image_process_12.png" alt="1% gel electrophoresis of plasmid" style="width:100%">
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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                <div class="mdl-card__supporting-text" style="font-size: 70%; position: absolute; bottom:0px; right:0px; color:#f0f0f0; text-align:right">
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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                  <!-- white:#f0f0f0 or black:#0F0F0F -->
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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                  pUAST-pleP-Gal4
−
</ul>
+
                </div>
 +
              </div>
 +
              <div class="demo-card-wide mdl-card mdl-shadow--2dp" style="width:30%; float:left; margin:0%; margin-right:5%">
 +
                <img src="https://static.igem.org/mediawiki/2017/e/e3/2017tongji_wiki_image_process_12.png" alt="1% gel electrophoresis of plasmid" style="width:100%">
 +
                <div class="mdl-card__supporting-text" style="font-size: 70%; position: absolute; bottom:0px; right:0px; color:#f0f0f0; text-align:right">
 +
                  <!-- white:#f0f0f0 or black:#0F0F0F -->
 +
                  pUAST-pleP-Gal80ts
 +
                </div>
 +
              </div>
 +
              <div class="demo-card-wide mdl-card mdl-shadow--2dp" style="width:30%; float:left; margin:0%;">
 +
                <img src="https://static.igem.org/mediawiki/2017/e/e3/2017tongji_wiki_image_process_12.png" alt="1% gel electrophoresis of plasmid" style="width:100%">
 +
                <div class="mdl-card__supporting-text" style="font-size: 70%; position: absolute; bottom:0px; right:0px; color:#f0f0f0; text-align:right">
 +
                  <!-- white:#f0f0f0 or black:#0F0F0F -->
 +
                  pUAST-UAS-TH
 +
                </div>
 +
              </div>
 +
            </div>
 +
          </div>
 +
          <div class="android-drawer-separator"></div>
 +
        </div>
  
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</div>
+
        <!-- Behavior Tests -->
 +
        <div class="demo-card-wide mdl-card mdl-shadow--2dp">
 +
          <div class="mdl-card__title" style="text-align:center">
 +
            <h4 class="mdl-card__title-text" style="font-size: 250%; color:#5a5a5a">Behavior Tests</h4>
 +
          </div>
 +
          <div class="mdl-card__supporting-text" style="font-size: 115%">
  
 +
            <b>Experiment 1: Use real-time PCR to determine whether the expression of TH is higher at 29°C</b><br><br>
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/0/08/2017tongji_wiki_image_result1.png" style="width:100%" alt="TH expression">
 +
              <div style="height:16px"></div>
 +
              Figure 1 - The relative expression of TH in modified fruit flies increased significantly.
 +
            </div>
 +
            <div class="android-drawer-separator"></div><br>
  
  
 +
            <b>Experiment 2: Detect male-male courtship when raising the temperature</b><br><br>
 +
            Mating index (the relative time that the fruit fly use for mating):
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/e/e4/2017tongji_wiki_image_result2.png" style="width:100%" alt="Mating Index">
 +
              <div style="height:16px"></div>
 +
              Figure 2 - The mating index of the treated group rises significantly in at 29°C.<br>
 +
              [time=5minutes, n=5, P<0.01]
 +
            </div>
 +
            <div class="android-drawer-separator"></div><br>
 +
 +
            <b>Experiment 3: Test the modified Drosophila’ gender preferences</b><br><br>
 +
            Comparison of time that the fruit flies use for mating male or female flies:
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/4/46/2017tongji_wiki_image_result3.png" style="width:100%" alt="male-male and male-female mating">
 +
              <div style="height:16px"></div>
 +
              Figure 3 - The modified fruit flies had no preference for males and females when mating.<br>
 +
              [time=10minutes, n=8, P>0.5]
 +
            </div>
 +
            <div class="android-drawer-separator"></div><br>
 +
 +
            <b>Experiment 4: Test whether the modified fruit flies increase their appeal for males</b><br><br>
 +
            The time that the fruit fly use for mating in five minutes:<br>
 +
            There was <b>no</b> courtship in all groups.
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <!-- <img src="" style="width:100%" alt=""> -->
 +
              <!-- <div style="height:16px"></div> -->
 +
              <!-- There was <b>no</b> courtship in all groups.<br> -->
 +
              [time=5minutes, n=5]
 +
            </div>
 +
            <div class="android-drawer-separator"></div><br>
 +
 +
            <b>Experiment 5: Does the co-culture of wild-type and modified fruit flies influence their courtship with wild-type females?</b><br><br>
 +
            Comparison of time that the fruit flies use for mating male or female flies:
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/7/76/2017tongji_wiki_image_result4.png" style="width:100%" alt="male-male and male-female mating">
 +
              <div style="height:16px"></div>
 +
              Figure 4 - The co-culture of wild-type and modified fruit flies did not affect the courtship of wild-type flies.<br>
 +
              [time=5minutes, n=5, P>0.5]
 +
            </div>
 +
            <div class="android-drawer-separator"></div><br>
 +
 +
            <b>Experiment 6: Is the viability of modified fruit flies affected?</b><br><br>
 +
            The number of live flies in the absence of food and water:
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/e/e7/2017tongji_wiki_image_result5.png" style="width:100%" alt="survival without food or water">
 +
              <div style="height:16px"></div>
 +
              Figure 5 - The survival curve of fruit flies in the absence of food and water
 +
            </div>
 +
            The number of live flies in high temperatures (37°C):
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/c/ce/2017tongji_wiki_image_result6.png" style="width:100%" alt="survival in high temperature">
 +
              <div style="height:16px"></div>
 +
              Figure 6 - The survival curve of fruit flies under high temperature<br>
 +
              [T=37°C]
 +
            </div>
 +
            The seismo-tube experiment:
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/5/55/2017tongji_wiki_image_result7.png" style="width:100%" alt="survival without food or water">
 +
              <div style="height:16px"></div>
 +
              Figure 7 - The modified fruit flies crawl higher in the seismo-tube experiment
 +
            </div>
 +
            <div class="android-drawer-separator"></div><br>
 +
 +
            <b>Experiment 7: The reproductive curve of the modified fruit fly.</b><br><br>
 +
            <div class="mdl-card__supporting-text" style="font-size: 75%; text-align:center">
 +
              <img src="https://static.igem.org/mediawiki/2017/c/c4/2017tongji_wiki_image_result8.png" style="width:100%" alt="male-male and male-female mating">
 +
              <div style="height:16px"></div>
 +
              Figure 8 - The reproduction curve of the modified fruit fly<br>
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              [n=5]
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            </div>
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            <div class="android-drawer-separator"></div><br>
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            <b>Experiment 8: Does the proportion of modified flies have an impact on mating rates of population?</b><br><br>
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              <img src="https://static.igem.org/mediawiki/2017/4/4f/2017tongji_wiki_image_result9.png" style="width:100%" alt="male-male and male-female mating">
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              <div style="height:16px"></div>
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              Figure 9 - The impact that proportion of modified flies have on mating rates of population<br>
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              [n=5]
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Revision as of 14:28, 29 October 2017


Tongji iGEM - InterLab
Tongji iGEM
TongJi iGEM
Results
The outcomes of our experiments.
expand_more

Plasmid Construction

We constructed three plasmid successfully: pUAST-pleP-Gal4, pUAST-pleP-Gal80ts and pUAST-UAS-TH.
This is our digest verification map:

1% gel electrophoresis of plasmid
pUAST-pleP-Gal4
1% gel electrophoresis of plasmid
pUAST-pleP-Gal80ts
1% gel electrophoresis of plasmid
pUAST-UAS-TH

Behavior Tests

Experiment 1: Use real-time PCR to determine whether the expression of TH is higher at 29°C

TH expression
Figure 1 - The relative expression of TH in modified fruit flies increased significantly.

Experiment 2: Detect male-male courtship when raising the temperature

Mating index (the relative time that the fruit fly use for mating):
Mating Index
Figure 2 - The mating index of the treated group rises significantly in at 29°C.
[time=5minutes, n=5, P<0.01]

Experiment 3: Test the modified Drosophila’ gender preferences

Comparison of time that the fruit flies use for mating male or female flies:
male-male and male-female mating
Figure 3 - The modified fruit flies had no preference for males and females when mating.
[time=10minutes, n=8, P>0.5]

Experiment 4: Test whether the modified fruit flies increase their appeal for males

The time that the fruit fly use for mating in five minutes:
There was no courtship in all groups.
[time=5minutes, n=5]

Experiment 5: Does the co-culture of wild-type and modified fruit flies influence their courtship with wild-type females?

Comparison of time that the fruit flies use for mating male or female flies:
male-male and male-female mating
Figure 4 - The co-culture of wild-type and modified fruit flies did not affect the courtship of wild-type flies.
[time=5minutes, n=5, P>0.5]

Experiment 6: Is the viability of modified fruit flies affected?

The number of live flies in the absence of food and water:
survival without food or water
Figure 5 - The survival curve of fruit flies in the absence of food and water
The number of live flies in high temperatures (37°C):
survival in high temperature
Figure 6 - The survival curve of fruit flies under high temperature
[T=37°C]
The seismo-tube experiment:
survival without food or water
Figure 7 - The modified fruit flies crawl higher in the seismo-tube experiment

Experiment 7: The reproductive curve of the modified fruit fly.

male-male and male-female mating
Figure 8 - The reproduction curve of the modified fruit fly
[n=5]

Experiment 8: Does the proportion of modified flies have an impact on mating rates of population?

male-male and male-female mating
Figure 9 - The impact that proportion of modified flies have on mating rates of population
[n=5]
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Find out more chevron_right