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| | <div class="hero-text"> | | <div class="hero-text"> |
| | <h1>Project Description</h1> | | <h1>Project Description</h1> |
| − | <p><b>A Visual, Mathematical Representation of Our Project</p></b>
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| | <div class="text"> | | <div class="text"> |
| | <h2>Description</h2> | | <h2>Description</h2> |
| | <p>Each year Enterotoxigenic Escherichia coli (ETEC), a form of pathogenic <I>E. coli</I> known for causing traveler’s stomach, affects millions of travelers. ETEC produces two toxins, a heat labile and a heat stable version. The basis of the project is to complete three different steps: sense ETEC, respond to its presence, and package the sense/response module in a cellulose matrix.</p> | | <p>Each year Enterotoxigenic Escherichia coli (ETEC), a form of pathogenic <I>E. coli</I> known for causing traveler’s stomach, affects millions of travelers. ETEC produces two toxins, a heat labile and a heat stable version. The basis of the project is to complete three different steps: sense ETEC, respond to its presence, and package the sense/response module in a cellulose matrix.</p> |
| − | <p>The goal of the sensing step is to engineer a non-pathogenic <I>E. coli</I> to detect the pathogenic <I>E.coli</I> communication signals. The response section involves engineering the same <I>E. coli</I> to glow green upon the detection of harmful bacteria. In order to package the sense and respond <I>E. coli</I>, cellulose binding domains will be engineered to be expressed on the Csg A portions of curli surface proteins on <I>E. coli</I>.</p> | + | |
| − | <p>We chose this project after brainstorming many problems that the United States Air Force airmen face on a daily basis. Traveling far and wide for months at a time, those deployed become susceptible to many pathogens in the water supply, especially harmful strains of <I>E. coli</I>. As ETEC is the most common cause of traveler's stomach, we can help to detect and using this modular system, potentially eradicate the pathogens from the water and the body. </p> | + | <p>The goal of the sensing step is to engineer a non-pathogenic <I>E. coli</I> to detect the pathogenic <I>E. coli</I> communication signals. The response section involves engineering the same <I>E. coli</I> to glow green upon the detection of harmful bacteria. In order to package the sense and respond <I>E. coli</I>, cellulose binding domains will be engineered to be expressed on the CsgA portions of curli specific proteins on <I>E. coli</I>.</p> |
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| + | <p>We chose this project after brainstorming many problems that United States Air Force personnel face on a daily basis. Traveling for months at a time, those deployed become susceptible to many pathogens in the water supply, especially harmful strains of <I>E. coli</I>. ETEC is the most common cause of traveler's stomach. We can help detect ETEC by using this modular system, potentially eradicating the pathogens from the water and the body.</p> |
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| − | <br> | + | <img src="https://static.igem.org/mediawiki/2017/thumb/e/ef/TDPie.png/800px-TDPie.png" style="height: auto; width: 60%; margin-right: 20%; margin-left: 20%;"> |
| − | <br> | + | <p class="caption" style="width=70%; margin-left: 15%; margin-right: 15%;" >Figure 1 Graph showing the percent of personnel who experience diarrhea during deployment and number of episodes experienced</p> |
| − | <br> | + | <img src="https://static.igem.org/mediawiki/2017/thumb/5/58/TDImpact2.png/800px-TDImpact2.png" style="height: auto; width: 60%; margin-right: 20%; margin-left: 20%;"> |
| − | <br><br> | + | <p class="caption" style="width=70%; margin-left: 15%; margin-right: 15%;" >Figure 2 This chart shows the results of a survey of deployed personnel who experienced diarrhea during deployment concerning its impact on performance</p> |
| − | <br> | + | <img src="https://static.igem.org/mediawiki/2017/3/30/TDCause2.png" style="height: auto; width: 60%; margin-right: 20%; margin-left: 20%;"> |
| | + | <p class="caption" style="width=70%; margin-left: 15%; margin-right: 15%;" >Figure 3 Chart showing the causes of diarrheal illness; the outer ring displays causes for deployed military and the inner for civilian travelers</p> |
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| | {{US_AFRL_CarrollHS_Nav}} | | {{US_AFRL_CarrollHS_Nav}} |
