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| <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li> | | <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstrate</a></li> |
| <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li> | | <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Applied_Design">Applied design</a></li> |
| + | <li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li> |
| + | |
| <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li> | | <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li> |
| | | |
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| </li> | | </li> |
| | | |
− | <li><a href="https://2017.igem.org/Team:CCU_Taiwan/InterLab">InterLab</a></li> | + | <li><a href="https://2017.igem.org/Team:CCU_Taiwan/Medals">Medals</a></li> |
| | | |
| </ul> | | </ul> |
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| | | |
| <div class="div_nav"> | | <div class="div_nav"> |
− | <nav class="toc_nav" id="toc_show"> | + | <nav style="top: 16vh !important;" class="toc_nav" id="toc_show"> |
| <ul> | | <ul> |
| <li> | | <li> |
− | <a href="#Fluorescein">Fluorescein curve</a> | + | <a href="#Week1">Week 01</a> |
− | <ul> | + | |
− | <li><a href="#Fluorescein-Plate-reader">Plate reader</a></li>
| + | |
− | <li><a href="#Fluorescein-Material">Material</a></li>
| + | |
− | <li><a href="#Fluorescein-Method">Method</a></li>
| + | |
− | <li><a href="#Fluorescein-Data-result">Data result</a></li>
| + | |
− | </ul>
| + | |
| </li> | | </li> |
− | <li> | + | |
− | <a href="#OD600">OD600 Reference point</a> | + | <li> |
− | <ul>
| + | <a href="#Week2">Week 02</a> |
− | <li><a href="#OD600-Plate-reader">Plate reader</a></li>
| + | |
− | <li><a href="#OD600-Material">Material</a></li>
| + | |
− | <li><a href="#OD600-Method">Method</a></li>
| + | |
− | <li><a href="#OD600-Data-result">Data result</a></li>
| + | |
− | </ul>
| + | |
| </li> | | </li> |
− | | + | <li> |
− | <li> | + | <a href="#Week3">Week 03</a> |
− | <a href="#Cell">Cell measure</a> | + | |
− | <ul>
| + | </li> |
− | <li><a href="#Cell-Material">Material</a></li>
| + | <li> |
− | <li><a href="#Cell-Method">Method</a></li>
| + | <a href="#Week4">Week 04</a> |
− | <li><a href="#Cell-Data-result">Data result</a></li>
| + | |
− | </ul>
| + | </li> |
| + | <li> |
| + | <a href="#Week5">Week 05</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week6">Week 06</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week7">Week 07</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week8">Week 08</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week9">Week 09</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week10">Week 10</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week11">Week 11</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week12">Week 12</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week13">Week 13</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week14">Week 14</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week15">Week 15</a> |
| + | |
| + | </li> |
| + | <li> |
| + | <a href="#Week16">Week 16</a> |
| + | |
| </li> | | </li> |
− |
| |
| | | |
| </ul> | | </ul> |
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| <article class="contents_nav"> | | <article class="contents_nav"> |
| <section> | | <section> |
− | | + | <div id="Week1"> |
| + | <div class="aaa"></div> |
| <h3>Week 01 (2017/07/02~2017/07/08)</h3> | | <h3>Week 01 (2017/07/02~2017/07/08)</h3> |
| <ol><li>Lactate detection system</li></ol> | | <ol><li>Lactate detection system</li></ol> |
− | <p> Gene design of lactate detector</p> | + | <p> Gene design of lactate detector</p> |
| <ol><li>CSP detection system</li></ol> | | <ol><li>CSP detection system</li></ol> |
− | <p> Gene design of CSP detector</p> | + | <p> Gene design of CSP detector</p> |
| + | </div> |
| | | |
| + | <div id="Week2"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 02 (2017/07/09~2017/07/15)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Gene design of lactate detector</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Gene design of CSP detector<br/> |
| + | Request 2 backbone plasmids of <i>Bacillus subtilis</i> from iGEM |
| + | </p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;border-color:#ccc;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#ccc;color:#333;background-color:#fff;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;border-color:#ccc;color:#333;background-color:#f0f0f0;} |
| + | .tg .tg-yw4l{vertical-align:top} |
| + | .tg .tg-b7b8{background-color:#f9f9f9;vertical-align:top} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-yw4l">Part requesting #1</th> |
| + | <th class="tg-yw4l">BBa_K823022</th> |
| + | <th class="tg-yw4l">pSBBs4S</th> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-b7b8">Part requesting #2</td> |
| + | <td class="tg-b7b8">BBa_K823023</td> |
| + | <td class="tg-b7b8">pSBBs1C</td> |
| + | </tr> |
| + | </table> |
| + | <p> Order chemical materials for preparing competent cell of <i>Bacillus subtilis</i></p> |
| + | </div> |
| + | |
| + | <div id="Week3"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 03 (2017/07/16~2017/07/22)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Gene design of lactate detector</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Gene design of CSP detector</p> |
| + | </div> |
| + | |
| + | <div id="Week4"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 04 (2017/07/23~2017/07/29)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Send orders to IDT for DNA synthesis</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Gene design of CSP detector</p> |
| + | </div> |
| + | |
| + | |
| + | <div id="Week5"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 05 (2017/07/30~2017/08/05)</h3> |
| + | <ol><li>Participate the 5th iGEM Asia-Pacific Conference at NCTU</li></ol> |
| + | |
| + | </div> |
| + | |
| + | |
| + | <div id="Week6"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 06 (2017/08/06~2017/08/12)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Prepare competent cell of <i>Bacillus subtilis</i><br/> |
| + | - Amplify two backbone plasmids of <i>Bacillus subtilis</i> |
| + | </p> |
| + | <ol><li>InterLab Study</li></ol> |
| + | <p> Transform eight kinds of plasmids into <i>E. coli DH5α</i></p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg .tg-yw4l{vertical-align:top} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">Positive Control</th> |
| + | <th class="tg-031e">BBa_I20270</th> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">Negative Control</td> |
| + | <td class="tg-031e">BBa_R0040</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 1</td> |
| + | <td class="tg-yw4l">BBa_J364000</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 2</td> |
| + | <td class="tg-yw4l">BBa_J364001</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 3</td> |
| + | <td class="tg-yw4l">BBa_J364002</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 4</td> |
| + | <td class="tg-yw4l">BBa_J364003</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 5</td> |
| + | <td class="tg-yw4l">BBa_J364004</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-yw4l">Test Device 6</td> |
| + | <td class="tg-yw4l">BBa_J364005</td> |
| + | </tr> |
| + | </table> |
| + | <p> Plate culture eight kinds of <i>E. coli DH5α</i></p> |
| + | </div> |
| + | |
| + | |
| + | <div id="Week7"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 07 (2017/08/13~2017/08/19)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Amplify the IDT plasmid of lactate detection system</p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">IDT plasmid # 1</th> |
| + | <th class="tg-031e">LldR gene on IDT vector</th> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">IDT plasmid # 2</td> |
| + | <td class="tg-031e">GFP gene on IDT vector</td> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">IDT plasmid # 3</td> |
| + | <td class="tg-031e">MazEF gene on IDT vector</td> |
| + | </tr> |
| + | </table> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Amplify the IDT plasmid of CSP detection system</p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">IDT plasmid # 1</th> |
| + | <th class="tg-031e">ComE gene on IDT vector</th> |
| + | </tr> |
| + | </table> |
| + | </div> |
| + | |
| + | <div id="Week8"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 08 (2017/08/20~2017/08/26)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Restriction map analysis of IDT Plasmid</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Restriction map analysis of <i>B. subtilis</i> backbone plasmid</p> |
| + | </div> |
| + | |
| + | |
| + | <div id="Week9"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 09 (2017/08/27~2017/09/02)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Function test of kill switch (mazEF)</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Transformation test of pSBBs1C and pSBBs4S</p> |
| + | </div> |
| + | |
| + | <div id="Week10"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 10 (2017/09/03~2017/09/09)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Amplify the backbone plasmid pSB1A2</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Transformation test of pSBBs1C and pSBBs4S<br/> |
| + | Amplify the IDT plasmid of CSP detection system |
| + | </p> |
| + | <style type="text/css"> |
| + | .tg {border-collapse:collapse;border-spacing:0;} |
| + | .tg td{font-family:Arial, sans-serif;font-size:14px;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | .tg th{font-family:Arial, sans-serif;font-size:14px;font-weight:normal;padding:10px 5px;border-style:solid;border-width:1px;overflow:hidden;word-break:normal;} |
| + | </style> |
| + | <table class="tg"> |
| + | <tr> |
| + | <th class="tg-031e">IDT plasmid # 2</th> |
| + | <th class="tg-031e">ComD gene on IDT plasmid</th> |
| + | </tr> |
| + | <tr> |
| + | <td class="tg-031e">IDT plasmid # 3</td> |
| + | <td class="tg-031e">GFP gene on IDT plasmid</td> |
| + | </tr> |
| + | </table> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> |
| + | Small scale culture with test paper<br/> |
| + | Freeze dry the test paper |
| + | </p> |
| + | </div> |
| + | |
| + | |
| + | <div id="Week11"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 11 (2017/09/10~2017/09/16)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Restriction map analysis of pSB1A2</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> Restriction map analysis of IDT plasmid</p> |
| + | <ol><li>InterLab study</li></ol> |
| + | <p> |
| + | Construct OD600 reference point<br/> |
| + | Construct fluorescence standard curve |
| + | </p> |
| + | </div> |
| + | |
| + | <div id="Week12"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 12 (2017/09/17~2017/09/23)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | pSB1A2_GFP_LldR assembly<br/> |
| + | pSB1C3_mazEF assembly |
| + | </p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> pSBBS1C_comE assembly</p> |
| + | <ol><li>InterLab study</li></ol> |
| + | <p> OD600 and fluorescence measurement of cell</p> |
| + | </div> |
| + | |
| + | <div id="Week13"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 13 (2017/09/24~2017/09/30)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | Restriction map analysis of recombination DNA</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> pSBBs1C_comD assembly</p> |
| + | <ol><li>InterLab study</li></ol> |
| + | <p> |
| + | E-mail InterLab data to iGEM<br/> |
| + | Fill out three online form of InterLab |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> |
| + | Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper<br/> |
| + | Freeze dry the test paper |
| + | </p> |
| + | </div> |
| + | |
| + | <div id="Week14"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 14 (2017/10/01~2017/10/07)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | Function test of lactate detector<br/> |
| + | Function test of kill switch |
| + | </p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | pSBBs1C_comE_comD assembly<br/> |
| + | pSBBs4S_GFP assembly |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> |
| + | Culture the bacteria with 600 mL LB in Erlenmeyer flask which contains test paper<br/> |
| + | Freeze dry the liquid culture |
| + | </p> |
| + | </div> |
| + | |
| + | <div id="Week15"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 15 (2017/10/08~2017/10/14)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> |
| + | Double plasmid transformation test<br/> |
| + | Function test of lactate detector<br/> |
| + | Function test of kill switch |
| + | </p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Check the size of assembly plasmid<br/> |
| + | Transform the recombination plasmid to <i>B. subtilis</i> |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> Revive the freeze-dried bacteria</p> |
| + | </div> |
| + | |
| + | |
| + | <div id="Week16"> |
| + | <div class="aaa"></div> |
| + | <h3>Week 16 (2017/10/15~2017/10/21)</h3> |
| + | <ol><li>Lactate detection system</li></ol> |
| + | <p> Bacteria which contain two plasmid</p> |
| + | <ol><li>CSP detection system</li></ol> |
| + | <p> |
| + | Prepare competent cell of <i>B. subtilis</i><br/> |
| + | Transform the recombination plasmid to <i>B. subtilis</i> |
| + | </p> |
| + | <ol><li>Test paper experiment</li></ol> |
| + | <p> Freeze dry the liquid culture, and then revive it</p> |
| + | </div> |
| </section> | | </section> |
| | | |
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| <!-- Contact --> | | <!-- Contact --> |
− | <section class="contact"> | + | |
− |
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− | <ul class="icons">
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− | <a href="emailto:ccu.igem.2017@gmail.com">
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− | </section>
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| | | |
| <!-- Copyright --> | | <!-- Copyright --> |
| <div class="copyright"> | | <div class="copyright"> |
− | <ul class="menu"> | + | |
− | <li>© 2017 CCU Taiwan iGEM</li><li>Design: <a href="http://html5up.net">HTML5 UP</a></li>
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− | </ul>
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| </div> | | </div> |
− | | + | <img src="https://static.igem.org/mediawiki/2017/f/fb/Ccu.jpg" width="400" height="120" style="margin-top: 5em; |
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| </div> | | </div> |
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| </div> | | </div> |
| + | |
| + | <!-- Scripts --> |
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