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| − | <div class="column full_size judges-will-not-evaluate">
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| − | <h3>★ ALERT! </h3>
| + | background-image: url("https://static.igem.org/mediawiki/2017/9/9c/US_AFRL_CarrollHS_BasicPart1.jpg");} |
| − | <p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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| − | <p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p> | + | |
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| | + | <h1>Basic Parts</h1> |
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| | + | <h2>csgA-dCBD </h2> |
| | + | <h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522000">csgA-dCBD (Curli fibers attachable to cellulose) BBa_K2522000</a></h3> |
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| − | | + | <p>BBa_K2522000 is a plasmid containing a genetic sequence that expresses CsgA and dCBD continuously. The final product was developed in several steps. A part from the iGEM Distribution Kit, BBa_K1321340, was used. This part is the genetic code for a double cellulose binding domain, which functions to attach an organism to cellulose. The second piece of genetic code used was the <i>csgA</i> gene. </p> |
| − | <h1>Basic Parts</h1> | + | <p><i>csg</i> (curli specific gene) followed by a letter of the alphabet refers to the genes involved in expressing curli fibers. Curli fibers are hair-like strands of proteins generated by certain strains of <i>E. coli</i> on the outer membrane of the bacteria. These proteins encompass the bacteria in a fibrous mesh. <i>csgA</i> in particular indicates the individual building blocks of the fibers.</p> |
| − | | + | <p>To construct the part, the genetic code for <i>csgA</i> was removed from the genomic DNA of <i>E. coli</i> Nissle and the dCBD was taken from the Distribution Kit. The genes were combined in a plasmid with the stop codon removed from the code for <i>csgA</i>. Using this process, the <i>csgA</i> flows directly into the dCBD, so that every time a CsgA unit is produced, a dCBD unit is attached to the <i>csgA</i>. Because of this, the mesh of curli fibers that encompasses the microbe can bind to cellulose so that the microbe is encapsulated in cellulose. |
| − | <p> | + | |
| − | A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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| | </p> | | </p> |
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| − | <p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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| − | <h3>Best Basic Part Special Prize</h3>
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| − | <p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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| − | <b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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| − | <h4>Note</h4>
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| − | <p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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