Difference between revisions of "Team:Glasgow/InterLab"

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Glasgow iGEM 2017

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Overview

Fluorescence is a commonly used method for detecting and quantitating gene expression. The InterLab aims to create a more uniform way of measuring fluorescence so that results from different labs can be compared. The results in this study will be collated with all other results for the InterLab study by iGEM HQ. From the results, it is seen that J23101 is the strongest promoter followed by J23106 and J23117 being the weakest, and B0034 is the stronger of the two ribosome binding sites with J364100 being the weaker. Additionally, we chose to investigate how commercial DH5α Esherichia coli derivatives and dimerisation of plasmids can affect gene expression, full methodology and results for this can be seen on the Measurement page.

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Aims

Aim 1
- Sub-aim 1
- Sub-aim 2

Aim 2
- Sub-aim 1
- Sub-aim 2

Materials and Methods

Condition set up

Sample preparation

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2
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Table 1: Optical density analysis of S. thermophilus growth

Results

Discussion

References

↑ Kiliç, A. O., Pavlova, S. I., Ma, W. G. & Tao, L. 1996. Analysis of Lactobacillus phages and bacteriocins in American dairy products and characterization of a phage isolated from yogurt. Appl Environ Microbiol, 62, 2111-6.

[1] Kiliç, A. O., Pavlova, S. I., Ma, W. G. & Tao, L. 1996. Analysis of Lactobacillus phages and bacteriocins in American dairy products and characterization of a phage isolated from yogurt. Appl Environ Microbiol, 62, 2111-6.

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Revision as of 20:43, 24 October 2017 (view source) Hannah-Tee (Talk \| contribs) ← Older edit		Revision as of 17:10, 25 October 2017 (view source) Hannah-Tee (Talk \| contribs) Newer edit →
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	==Overview==		==Overview==

−	Fluorescence is a commonly used method for detecting and quantitating gene expression. The InterLab aims to create a more uniform way of measuring fluorescence so that results from different labs can be compared. The results in this study will be collated with all other results for the InterLab study by iGEM HQ. From the results, it is seen that J23101 is the strongest promoter followed by J23106 and J23117 being the weakest, and B0034 is the stronger of the two ribosome binding sites with J364100 being the weaker. Additionally, ~~it appears that plasmid dimerisation can greatly affect the results when studying gene expression and should be screened for and avoided. Finally, there seems~~ to ~~also be a difference between fluorescence values measured in~~ <i>~~Escherichia~~ coli</i> ~~strains NEB5α~~ and ~~DH5α~~.	+	Fluorescence is a commonly used method for detecting and quantitating gene expression. The InterLab aims to create a more uniform way of measuring fluorescence so that results from different labs can be compared. The results in this study will be collated with all other results for the InterLab study by iGEM HQ. From the results, it is seen that J23101 is the strongest promoter followed by J23106 and J23117 being the weakest, and B0034 is the stronger of the two ribosome binding sites with J364100 being the weaker. Additionally, we chose to investigate how commercial DH5α <i> Esherichia coli </i> derivatives and dimerisation of plasmids can affect gene expression, full methodology and results for this can be seen on the [https://2017.igem.org/Team:Glasgow/Measurement Measurement] page.
		+