Difference between revisions of "Team:US AFRL CarrollHS/Experiments"

Line 88: Line 88:
 
<li>Thaw 50 µL vial of BL21 cells on ice</li>
 
<li>Thaw 50 µL vial of BL21 cells on ice</li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells<li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells<li>
−
<l>incubate on ice for 30 minutes</li>
+
<li>incubate on ice for 30 minutes</li>
 
<li.b>heat shock at 42℃ for 10 seconds</li>
 
<li.b>heat shock at 42℃ for 10 seconds</li>
 
<li.b>incubate on ice for 5m</li>
 
<li.b>incubate on ice for 5m</li>

Revision as of 21:57, 24 October 2017


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
  4. incubate on ice for 30 minutes
    5. heat shock at 42℃ for 10 seconds incubate on ice for 5m
  5. Inoculate 250µL LB into vial
    6. incubate at 37℃ with shaking for 1.5 hours
  6. Plate 1x/9x

Methodology