Difference between revisions of "Team:US AFRL CarrollHS/Experiments"

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<li>Inoculate 2µL DNA into 50µL BL21 cells</li>
 
<li>Inoculate 2µL DNA into 50µL BL21 cells</li>
 
<li>incubate on ice for 30 minutes</li>
 
<li>incubate on ice for 30 minutes</li>
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<li class="b">heat shock at 42℃ for 10 seconds</li>
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<ul>
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<li class="b">incubate on ice for 5m</li>
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<li>heat shock at 42℃ for 10 seconds</li>
 +
<li>incubate on ice for 5m</li>
 +
</ul>
 
<li>Inoculate 250µL LB into vial</li>
 
<li>Inoculate 250µL LB into vial</li>
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<li class="b">incubate at 37℃ with shaking for 1.5 hours</li>
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<ul>
 +
<li>incubate at 37℃ with shaking for 1.5 hours</li>
 +
</ul>
 
<li>Plate 1x/9x</li>
 
<li>Plate 1x/9x</li>
 
</ol>
 
</ol>

Revision as of 23:00, 24 October 2017


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
  3. incubate on ice for 30 minutes
    • heat shock at 42℃ for 10 seconds
    • incubate on ice for 5m
  4. Inoculate 250µL LB into vial
    • incubate at 37℃ with shaking for 1.5 hours
  5. Plate 1x/9x

Methodology