Difference between revisions of "Team:Tongji China/Results"
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<b>Experiment 1: Use real-time PCR to determine whether the expression of TH is higher at 29°C</b><br><br> | <b>Experiment 1: Use real-time PCR to determine whether the expression of TH is higher at 29°C</b><br><br> | ||
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<img src="https://static.igem.org/mediawiki/2017/0/08/2017tongji_wiki_image_result1.png" class="imagelayout chartopacity" alt="TH expression"> | <img src="https://static.igem.org/mediawiki/2017/0/08/2017tongji_wiki_image_result1.png" class="imagelayout chartopacity" alt="TH expression"> | ||
<div style="height:16px"></div> | <div style="height:16px"></div> | ||
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<b>Experiment 2: Detect male-male courtship when raising the temperature</b><br><br> | <b>Experiment 2: Detect male-male courtship when raising the temperature</b><br><br> | ||
Mating index (the relative time that the fruit fly use for mating): | Mating index (the relative time that the fruit fly use for mating): | ||
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<img src="https://static.igem.org/mediawiki/2017/e/e4/2017tongji_wiki_image_result2.png" class="imagelayout chartopacity" alt="Mating Index"> | <img src="https://static.igem.org/mediawiki/2017/e/e4/2017tongji_wiki_image_result2.png" class="imagelayout chartopacity" alt="Mating Index"> | ||
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<b>Experiment 3: Test the modified Drosophila’ gender preferences</b><br><br> | <b>Experiment 3: Test the modified Drosophila’ gender preferences</b><br><br> | ||
Comparison of time that the fruit flies use for mating male or female flies: | Comparison of time that the fruit flies use for mating male or female flies: | ||
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<img src="https://static.igem.org/mediawiki/2017/4/46/2017tongji_wiki_image_result3.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | <img src="https://static.igem.org/mediawiki/2017/4/46/2017tongji_wiki_image_result3.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | ||
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The time that the fruit fly use for mating in five minutes:<br> | The time that the fruit fly use for mating in five minutes:<br> | ||
There was <b>no</b> courtship in all groups. | There was <b>no</b> courtship in all groups. | ||
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<b>Experiment 5: Does the co-culture of wild-type and modified fruit flies influence their courtship with wild-type females?</b><br><br> | <b>Experiment 5: Does the co-culture of wild-type and modified fruit flies influence their courtship with wild-type females?</b><br><br> | ||
Comparison of time that the fruit flies use for mating male or female flies: | Comparison of time that the fruit flies use for mating male or female flies: | ||
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<img src="https://static.igem.org/mediawiki/2017/7/76/2017tongji_wiki_image_result4.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | <img src="https://static.igem.org/mediawiki/2017/7/76/2017tongji_wiki_image_result4.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | ||
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<b>Experiment 6: Is the viability of modified fruit flies affected?</b><br><br> | <b>Experiment 6: Is the viability of modified fruit flies affected?</b><br><br> | ||
The number of live flies in the absence of food and water: | The number of live flies in the absence of food and water: | ||
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<img src="https://static.igem.org/mediawiki/2017/e/e7/2017tongji_wiki_image_result5.png" class="imagelayout chartopacity" alt="survival without food or water"> | <img src="https://static.igem.org/mediawiki/2017/e/e7/2017tongji_wiki_image_result5.png" class="imagelayout chartopacity" alt="survival without food or water"> | ||
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The number of live flies in high temperatures (37°C): | The number of live flies in high temperatures (37°C): | ||
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<img src="https://static.igem.org/mediawiki/2017/c/ce/2017tongji_wiki_image_result6.png" class="imagelayout chartopacity" alt="survival in high temperature"> | <img src="https://static.igem.org/mediawiki/2017/c/ce/2017tongji_wiki_image_result6.png" class="imagelayout chartopacity" alt="survival in high temperature"> | ||
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The seismo-tube experiment: | The seismo-tube experiment: | ||
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<img src="https://static.igem.org/mediawiki/2017/5/55/2017tongji_wiki_image_result7.png" class="imagelayout chartopacity" alt="survival without food or water"> | <img src="https://static.igem.org/mediawiki/2017/5/55/2017tongji_wiki_image_result7.png" class="imagelayout chartopacity" alt="survival without food or water"> | ||
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<b>Experiment 7: The reproductive curve of the modified fruit fly.</b><br><br> | <b>Experiment 7: The reproductive curve of the modified fruit fly.</b><br><br> | ||
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<img src="https://static.igem.org/mediawiki/2017/c/c4/2017tongji_wiki_image_result8.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | <img src="https://static.igem.org/mediawiki/2017/c/c4/2017tongji_wiki_image_result8.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | ||
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<b>Experiment 8: Does the proportion of modified flies have an impact on mating rates of population?</b><br><br> | <b>Experiment 8: Does the proportion of modified flies have an impact on mating rates of population?</b><br><br> | ||
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<img src="https://static.igem.org/mediawiki/2017/4/4f/2017tongji_wiki_image_result9.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | <img src="https://static.igem.org/mediawiki/2017/4/4f/2017tongji_wiki_image_result9.png" class="imagelayout chartopacity" alt="male-male and male-female mating"> | ||
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Revision as of 19:51, 29 October 2017
Tongji iGEM
TongJi iGEM
Results
The outcomes of our experiments.
expand_more
expand_more
Plasmid Construction
We constructed three plasmid successfully: pUAST-pleP-Gal4, pUAST-pleP-Gal80ts and pUAST-UAS-TH.
This is our digest verification map:
This is our digest verification map:
pUAST-pleP-Gal4
pUAST-pleP-Gal80ts
pUAST-UAS-TH
Behavior Tests
Experiment 1: Use real-time PCR to determine whether the expression of TH is higher at 29°C
Figure 1 - The relative expression of TH in modified fruit flies increased significantly.
Experiment 2: Detect male-male courtship when raising the temperature
Mating index (the relative time that the fruit fly use for mating):
Figure 2 - The mating index of the treated group rises significantly in at 29°C.
[time=5minutes, n=5, P<0.01]
Experiment 3: Test the modified Drosophila’ gender preferences
Comparison of time that the fruit flies use for mating male or female flies:
Figure 3 - The modified fruit flies had no preference for males and females when mating.
[time=10minutes, n=8, P>0.5]
Experiment 4: Test whether the modified fruit flies increase their appeal for males
The time that the fruit fly use for mating in five minutes:
There was no courtship in all groups.
Experiment 5: Does the co-culture of wild-type and modified fruit flies influence their courtship with wild-type females?
Comparison of time that the fruit flies use for mating male or female flies:
Figure 4 - The co-culture of wild-type and modified fruit flies did not affect the courtship of wild-type flies.
[time=5minutes, n=5, P>0.5]
Experiment 6: Is the viability of modified fruit flies affected?
The number of live flies in the absence of food and water:
Figure 5 - The survival curve of fruit flies in the absence of food and water
The number of live flies in high temperatures (37°C):
Figure 6 - The survival curve of fruit flies under high temperature
[T=37°C]
The seismo-tube experiment:
Figure 7 - The modified fruit flies crawl higher in the seismo-tube experiment
Experiment 7: The reproductive curve of the modified fruit fly.
Figure 8 - The reproduction curve of the modified fruit fly
[n=5]
Experiment 8: Does the proportion of modified flies have an impact on mating rates of population?
Figure 9 - The impact that proportion of modified flies have on mating rates of population
[n=5]
Figure 1 - The relative expression of TH in modified fruit flies increased significantly.
Experiment 2: Detect male-male courtship when raising the temperature
Mating index (the relative time that the fruit fly use for mating):
Figure 2 - The mating index of the treated group rises significantly in at 29°C.[time=5minutes, n=5, P<0.01]
Experiment 3: Test the modified Drosophila’ gender preferences
Comparison of time that the fruit flies use for mating male or female flies:
Figure 3 - The modified fruit flies had no preference for males and females when mating.[time=10minutes, n=8, P>0.5]
Experiment 4: Test whether the modified fruit flies increase their appeal for males
The time that the fruit fly use for mating in five minutes:
There was no courtship in all groups.
[time=5minutes, n=5]
Experiment 5: Does the co-culture of wild-type and modified fruit flies influence their courtship with wild-type females?
Comparison of time that the fruit flies use for mating male or female flies:
Figure 4 - The co-culture of wild-type and modified fruit flies did not affect the courtship of wild-type flies.[time=5minutes, n=5, P>0.5]
Experiment 6: Is the viability of modified fruit flies affected?
The number of live flies in the absence of food and water:
Figure 5 - The survival curve of fruit flies in the absence of food and water
Figure 6 - The survival curve of fruit flies under high temperature[T=37°C]
Figure 7 - The modified fruit flies crawl higher in the seismo-tube experiment
Experiment 7: The reproductive curve of the modified fruit fly.
Figure 8 - The reproduction curve of the modified fruit fly[n=5]
Experiment 8: Does the proportion of modified flies have an impact on mating rates of population?
Figure 9 - The impact that proportion of modified flies have on mating rates of population[n=5]
