Difference between revisions of "Team:US AFRL CarrollHS/Composite Part"

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<h2>Insert Header</h2>
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<h2>Part: PLsrA_sfGFP</h2>
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<p>The plasmid is a sense and response to Auto Inducer-2, a
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quorum sensing molecule, that is present among most ETEC strains.
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The constitutive promoter in PLsR creates LsrR, binding to AI-2 to turn
 +
on PLsR promoter site. Once quorum sensing signals are detected,
 +
sfGFP, super folded green fluorescent protein, production begins.
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PLsR promoter site in modified plasmid turns on the ribosomal switch
 +
and starts the production of sfGFP. sfGFP emits a visibly fluorescent
 +
green response and alerts the human of the presence of ETEC.
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Our part was adapted from Part BBa_K117008 pLsrA-YFP.</p>
 
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<h2>Insert Header</h2>
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<h2>Part: Plas_sfGFP</h2>
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<p>Insert Text</p>
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<p>Engineered to detect communication signals between the pathogenic
 +
E. coli strains. The first plasmid created with PLas_sfGFP promoter
 +
activated by 3OC12 in order to produce Green Fluorescent Protein
 +
(GFP) PLas promoter detects 30C12, a known quorum sensing
 +
molecule. The team used this plasmid as an initial experimentation
 +
using promoter sites and the observations of positive responses to
 +
quorum sensing molecules.</p>
  
 
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</div>

Revision as of 20:44, 31 October 2017


Composite Parts

Part: PLsrA_sfGFP

The plasmid is a sense and response to Auto Inducer-2, a quorum sensing molecule, that is present among most ETEC strains. The constitutive promoter in PLsR creates LsrR, binding to AI-2 to turn on PLsR promoter site. Once quorum sensing signals are detected, sfGFP, super folded green fluorescent protein, production begins. PLsR promoter site in modified plasmid turns on the ribosomal switch and starts the production of sfGFP. sfGFP emits a visibly fluorescent green response and alerts the human of the presence of ETEC. Our part was adapted from Part BBa_K117008 pLsrA-YFP.


Part: Plas_sfGFP

Engineered to detect communication signals between the pathogenic E. coli strains. The first plasmid created with PLas_sfGFP promoter activated by 3OC12 in order to produce Green Fluorescent Protein (GFP) PLas promoter detects 30C12, a known quorum sensing molecule. The team used this plasmid as an initial experimentation using promoter sites and the observations of positive responses to quorum sensing molecules.