Difference between revisions of "Team:US AFRL CarrollHS/Composite Part"

Line 19: Line 19:
 
<h2>Part: PLsrA_sfGFP</h2>
 
<h2>Part: PLsrA_sfGFP</h2>
 
<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522002">BBa_K2522002</a></h3>
 
<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522002">BBa_K2522002</a></h3>
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<p>The plasmid is a sense and response to Auto Inducer-2, a
+
<p>The plasmid senses and responds to Auto Inducer-2, a
 
quorum sensing molecule, that is present among most ETEC strains.
 
quorum sensing molecule, that is present among most ETEC strains.
 
The constitutive promoter in PLsR creates LsrR, binding to AI-2 to turn
 
The constitutive promoter in PLsR creates LsrR, binding to AI-2 to turn
 
on PLsR promoter site. Once quorum sensing signals are detected,
 
on PLsR promoter site. Once quorum sensing signals are detected,
 
sfGFP, super folded green fluorescent protein, production begins.
 
sfGFP, super folded green fluorescent protein, production begins.
−
PLsR promoter site in modified plasmid turns on the ribosomal switch
+
PLsR promoter site in the modified plasmid turns on the ribosomal switch
 
and starts the production of sfGFP. sfGFP emits a visibly fluorescent
 
and starts the production of sfGFP. sfGFP emits a visibly fluorescent
 
green response and alerts the human of the presence of ETEC.
 
green response and alerts the human of the presence of ETEC.
Line 35: Line 35:
 
<h2>Part: Plas_sfGFP</h2>
 
<h2>Part: Plas_sfGFP</h2>
 
<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522003">BBa_K2522003</a></h3>
 
<h3><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2522003">BBa_K2522003</a></h3>
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<p>Engineered to detect communication signals between the pathogenic
+
<p>The plasmid is engineered to detect communication signals between the pathogenic
−
E. coli strains. The first plasmid created with PLas_sfGFP promoter
+
E. coli strains. The first plasmid created with PLas_sfGFP promoter is
 
activated by 3OC12 in order to produce Green Fluorescent Protein
 
activated by 3OC12 in order to produce Green Fluorescent Protein
 
(GFP) PLas promoter detects 30C12, a known quorum sensing
 
(GFP) PLas promoter detects 30C12, a known quorum sensing

Revision as of 00:19, 1 November 2017


Composite Parts

Part: PLsrA_sfGFP

BBa_K2522002

The plasmid senses and responds to Auto Inducer-2, a quorum sensing molecule, that is present among most ETEC strains. The constitutive promoter in PLsR creates LsrR, binding to AI-2 to turn on PLsR promoter site. Once quorum sensing signals are detected, sfGFP, super folded green fluorescent protein, production begins. PLsR promoter site in the modified plasmid turns on the ribosomal switch and starts the production of sfGFP. sfGFP emits a visibly fluorescent green response and alerts the human of the presence of ETEC. Our part was adapted from Part BBa_K117008 pLsrA-YFP.


Part: Plas_sfGFP

BBa_K2522003

The plasmid is engineered to detect communication signals between the pathogenic E. coli strains. The first plasmid created with PLas_sfGFP promoter is activated by 3OC12 in order to produce Green Fluorescent Protein (GFP) PLas promoter detects 30C12, a known quorum sensing molecule. The team used this plasmid as an initial experimentation using promoter sites and the observations of positive responses to quorum sensing molecules.