Difference between revisions of "Team:US AFRL CarrollHS"

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	<h1>Engineered Microbes to Sense and Respond to ETEC</h1>		<h1>Engineered Microbes to Sense and Respond to ETEC</h1>
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	Every year, Enterotoxigenic Escherichia coli (ETEC), the most common form of traveler’s diarrhea, affects thousands of deployed warfighters. The goal is to engineer non-pathogenic <i>E. coli</i> to sense ETEC, respond to its presence, and package it in a cellulose matrix to enable environmental detection of ETEC. We created two plasmids: ‘sense-respond’; and ‘packaging’. The sense-respond plasmid sensed Auto-Inducer 2 (AI-2), a quorum sensing molecule created by most ETEC strains, by expressing LsrR which switches on the Lsr promoter. Activation of the Lsr promoter expresses Super-Folder Green Fluorescent Protein (sfGFP), indicating the presence of ETEC. The packaging plasmid expresses a fusion protein consisting of curli fibers and cellulose binding domains. These modified surface proteins permit the bacteria to bind to cellulose, encapsulating the sense-response module. We envision this genetically engineered machine to be deployed in both the internal and external environment to detect ETEC.		Every year, Enterotoxigenic Escherichia coli (ETEC), the most common form of traveler’s diarrhea, affects thousands of deployed warfighters. The goal is to engineer non-pathogenic <i>E. coli</i> to sense ETEC, respond to its presence, and package it in a cellulose matrix to enable environmental detection of ETEC. We created two plasmids: ‘sense-respond’; and ‘packaging’. The sense-respond plasmid sensed Auto-Inducer 2 (AI-2), a quorum sensing molecule created by most ETEC strains, by expressing LsrR which switches on the Lsr promoter. Activation of the Lsr promoter expresses Super-Folder Green Fluorescent Protein (sfGFP), indicating the presence of ETEC. The packaging plasmid expresses a fusion protein consisting of curli fibers and cellulose binding domains. These modified surface proteins permit the bacteria to bind to cellulose, encapsulating the sense-response module. We envision this genetically engineered machine to be deployed in both the internal and external environment to detect ETEC.
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Revision as of 03:00, 26 September 2017

• 
• Team
  Team Mentors Collaborations
• Project
  Description Interlab Design Experiments Notebook Contributions Modeling Results Improve Attributions
• Parts
  Parts Basic Parts Composite Parts
• Safety
• Human Practices
  Silver HP Integrated and Gold HP Public Engagement
• Judging
  Medal Criteria Judging Form
• Jamboree
  Awards Our Time in Boston Memes

The Whole Student Team!

Practicing presenting and rocking shades

Dr. O'Malley, Annie, Jonah, Dr. Dickey, and Dr. Carter modelling new shirts!

Productive meeting to work on wiki and presentation

Enjoying ice cream with RH lab mentor Mike!

❮ ❯

Engineered Microbes to Sense and Respond to ETEC

Abstract:

Every year, Enterotoxigenic Escherichia coli (ETEC), the most common form of traveler’s diarrhea, affects thousands of deployed warfighters. The goal is to engineer non-pathogenic E. coli to sense ETEC, respond to its presence, and package it in a cellulose matrix to enable environmental detection of ETEC. We created two plasmids: ‘sense-respond’; and ‘packaging’. The sense-respond plasmid sensed Auto-Inducer 2 (AI-2), a quorum sensing molecule created by most ETEC strains, by expressing LsrR which switches on the Lsr promoter. Activation of the Lsr promoter expresses Super-Folder Green Fluorescent Protein (sfGFP), indicating the presence of ETEC. The packaging plasmid expresses a fusion protein consisting of curli fibers and cellulose binding domains. These modified surface proteins permit the bacteria to bind to cellulose, encapsulating the sense-response module. We envision this genetically engineered machine to be deployed in both the internal and external environment to detect ETEC.