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Revision as of 21:29, 24 October 2017


Experiment

Transformations

BI21 Cells:

  1. Thaw 50 µL vial of BL21 cells on ice
  2. Inoculate 2µL DNA into 50µL BL21 cells
  3. incubate on ice for 30 minutes
    4. heat shock at 42℃ for 10 seconds incubate on ice for 5m
  4. Inoculate 250µL LB into vial
    5. incubate at 37℃ with shaking for 1.5 hours
  5. Plate 1x/9x


Methodology