Difference between revisions of "Team:Tongji China/Safety"

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             In our project, we use 2 kinds of organism, Drosophila melanogaster and Escherichia coli . E.coli is ranked in the organisms of Level 1 risk goup. And because D.melanogaster is not included in the iGEM White List, we contacted Safety committee, Gender Diversity committee and Human Practices committee positively and got permissions.<br>
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             In our project, we use 2 kinds of organism, Drosophila melanogaster and Escherichia coli . E.coli is ranked in the organisms of Level 1 risk goup. And because D.melanogaster is not included in the iGEM White List, we contacted Safety committee, Gender Diversity committee and Human Practices committee positively and got permissions.<br><br>
  
 
Even though the E.coli and we work with is not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaClO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.
 
Even though the E.coli and we work with is not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaClO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.
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We use glass tube with cotton plug to keep the fruit flies in the lab and different samples are placed in different incubators with safety lock. In the process of culturing fruit flies, the fruit flies can not escape the incubator.<br><br>
 
We use glass tube with cotton plug to keep the fruit flies in the lab and different samples are placed in different incubators with safety lock. In the process of culturing fruit flies, the fruit flies can not escape the incubator.<br><br>
  
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The lab is divided into several areas by function and separated by double-doors, for example student-resting area, inoculation area, experimental area and so on. The inoculation area is located at the innermost and its ventilation is carried out only by center ventilation system. Air in center ventilation system will be disposed of expertly so that the fruit flies escaping from incubator will be collected. <br><br>
 
The lab is divided into several areas by function and separated by double-doors, for example student-resting area, inoculation area, experimental area and so on. The inoculation area is located at the innermost and its ventilation is carried out only by center ventilation system. Air in center ventilation system will be disposed of expertly so that the fruit flies escaping from incubator will be collected. <br><br>
  
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Vinegar traps and sticky traps are placed in all the areas in order to prevent any escape of the flies in experiment. They mimic the smell of rotting fruits and attract fruit flies, flies will be stuck or drowned in the traps. <br><br>
 
Vinegar traps and sticky traps are placed in all the areas in order to prevent any escape of the flies in experiment. They mimic the smell of rotting fruits and attract fruit flies, flies will be stuck or drowned in the traps. <br><br>
  
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Revision as of 17:07, 28 October 2017


Tongji iGEM - InterLab
TongJi iGEM
TongJi iGEM
Safety is prior to all other stuffs
expand_more

1. Laboratory information

All the members of Tongji-China have always attached great importance to bio-safety. We think we should introduce our adviser Lei Xue, a professor of genetics at the school of life sciences at Tongji University. His research areas include the molecular and genetic mechanism of tumorigenesis and apoptosis with drosophila model. Professor Xue and other researchers in the lab supervise us and give us a lot of guidance in fruit fly experiment.

4th 2. Organism

In our project, we use 2 kinds of organism, Drosophila melanogaster and Escherichia coli . E.coli is ranked in the organisms of Level 1 risk goup. And because D.melanogaster is not included in the iGEM White List, we contacted Safety committee, Gender Diversity committee and Human Practices committee positively and got permissions.

Even though the E.coli and we work with is not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaClO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.

We attach great importance to the safety of Drosophila melanogaster experiments and learnt experimental operation earnestly in Xue's Lab. We have complete measures to prevent flies from escaping. After asking Xue's permission, we take photos to show how we keep the flies contained.

NO DESCRIPTION

We use glass tube with cotton plug to keep the fruit flies in the lab and different samples are placed in different incubators with safety lock. In the process of culturing fruit flies, the fruit flies can not escape the incubator.

NO DESCRIPTION NO DESCRIPTION NO DESCRIPTION