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− | <h2></h2> | + | <h2>Week 9: July 24-28</h2> |
| <h3>Monday (7/24/17)</h3> | | <h3>Monday (7/24/17)</h3> |
− | <p></p> | + | <p>The day was a bit more relaxed for the Monday morning. The weekly lab |
| + | meeting was scheduled and the students were able to learn a lot about other |
| + | researchers and their current studies. In addition, they learned how to present to |
| + | an audience and create simple but informational slides. Afterwards, they enjoyed |
| + | a team lunch together with mentors to discuss the Michigan meet up. The |
| | | |
− | <h3></h3>
| + | ecstatic students exchanged travel ideas and a powerpoint presentation for the "mini- |
− | <p></p>
| + | jamboree". Afterwards, the young researchers returned to the lab to pick colonies from Friday |
| | | |
− | <h3></h3>
| + | to grow overnight in LB-Chl. They then left for the afternoon to get on wi-fi and worked on the |
− | <p></p>
| + | wiki and human outreach goals.</p> |
| | | |
− | <h3></h3> | + | <h3>Tuesday (7/25/17)</h3> |
− | <p></p> | + | <p>The adventures continue the next morning as the team went back into the lab to |
| + | look at the overnight cultures. Tina and Hayley were extremely pleased to find |
| + | their cultures had grown perfectly and were ready to do the mini-prep and nano |
| + | drop. However, Dallas and Jason did not get favorable results. They were very |
| + | disappointed to find out that their cultures had not grown and would need to |
| + | restart and pick other colonies.</p> |
| | | |
− | <h3></h3> | + | <h3>Thursday (7/27/17)</h3> |
− | <p></p> | + | <p>Another meeting was held to discuss the presentation and viability of the |
| + | Michigan State Trip. Students from both labs went over their combined |
| + | presentation and rehearsed thoroughly. Later in the lab. Hayley and Tina prepared |
| + | cultures of GFPa1 and sfGFP from the plates. Dallas transformed the iGem |
| + | cultures but sadly failed. Dr. Goodson recommended that they regrow the plates |
| + | and proceed again tomorrow. Later in the day, the young researchers finalized their plan for |
| + | "flat Stanley" and the outreach survey they hope to do in the future.</p> |
| | | |
− | <h3></h3> | + | <h3>Friday (7/28/17)</h3> |
− | <p></p> | + | <p>The morning began bright and early with |
| + | a tour of a C-17 cargo plane on base. It |
| + | was a great bonding experience for |
| + | teams from both labs as they took |
| + | pictures and learned about the C-17 |
| + | aircraft. The RX group mentally prepared to present |
| + | their project later in the afternoon to some high-ups |
| + | and members of their lab. The RH Lab planned to |
| + | attend and give them support. The presentation |
| + | went very well and the RX lab members received a |
| + | stellar ovation. Later in the afternoon, the RH lab |
| + | would be preparing a mini-prep for the Well 3, 18K |
| + | competent cells provided by iGem. The students |
| + | had just found out yesterday that the cultures finally |
| + | worked and that they could start proceeding to the |
| + | next step, which was extremely exciting. As experts |
| + | in the lab now, the group was able to follow the |
| + | protocol with ease and completed the protocol |
| + | accurately and quickly.</p> |
| + | </div> |
| | | |
− | <h3></h3> | + | <div class="text2"> |
− | <p></p> | + | <h2>Week 10: July 31-August 4</h2> |
| + | <h3>Monday (7/31/17)</h3> |
| + | <p>The morning started with the students performing a digest over the mini prep |
| + | from several days pervious. Two digests were preformed using different sets of |
| + | cut enzymes. Digest 1 was using the SpnI-HF and BmrI, while Digest 2 used the |
| + | cut enzymes PacI and BmrI. The Digest was then confirmed using a gel, which |
| + | showed the accurate number of base pairs.</p> |
| + | |
| + | <h3>Tuesday (8/1/17)</h3> |
| + | <p>The students set up another run of their GFP Project. The students used a |
| + | Kinetics reading of the Plate Reader. The kinetics were taken over 24 hours, every |
| + | 20 minutes. The students then worked on the wiki, trying to create a template.</p> |
| + | |
| + | <h3>Wednesday (8/2/17)</h3> |
| + | <p>Today was an exciting day for the students as they got to perform their survey |
| + | at their high school. The iGEM students volunteered to help the high school on |
| + | schedule pick up day. As the other students were coming to grab their schedule |
| + | we would offer for them to fill out a pick survey on computers we had set up. |
| + | Over 200 students varying in with some teachers filled the survey. This was a |
| + | |
| + | huge success for the students.</p> |
| + | |
| + | <h3>Thursday (8/3/17)</h3> |
| + | <p>Today the students got back to work on the sense plasmid. The students |
| + | performed a PCR, because finally their primers came in. The PCR product was |
| + | ran on the gel and the students got the results they were hoping for. Next, they |
| + | performed a MiniElute Reaction Cleanup Kit and then conducted a Nanodrop. |
| + | The Nanodrop were high concentrations of DNA. To end the day, the students |
| + | |
| + | performed another digest using the PCR they had just purified.</p> |
| + | |
| + | <h3>Friday (8/4/17)</h3> |
| + | <p>The big day to present to the Chief Scientist of the lab had finally come. Early in |
| + | the day, the students had a dry presentation with their teachers and mentors. |
| + | They received criticism and praise for their work and presentation that they would |
| + | present later. They left in high spirits to return to the lab and begin the |
| + | purification, ligation, and transformation of the plasmid for the “sense” |
| + | component of the project. The purification was then nanodroped and the students were |
| + | pleased with the results. The students then performed a ligation on the plasmid backbone |
| + | from the iGEM part and the sfGFP. The ligation seemed to go as planned and the students |
| + | hoped that the two sticky ends had connected. After completing the transformation, they got |
| + | ready to present to the Chief Scientist who got the funding for the iGem team. The |
| + | presentation went extremely well and the Chief Scientist was very impressed with their work |
| + | in the summer and the progress made. The students were finally able to let out a sigh of |
| + | relief as they finished their presentation successfully. Afterwards, they came back to the lab |
| + | to perform the transformation on the plasmid. The students followed the protocol exactly |
| + | and were able to achieve favorable results.</p> |
| + | </div> |
| + | |
| + | <div class="text"> |
| + | <h2>Week 11: August 7-11</h2> |
| + | <h3>Monday (8/7/17)</h3> |
| + | <p>Unfortunately the students were disheartened to learn that none of the plates |
| + | grew colonies. The students were at first shocked, but immediately started |
| + | tracing back to the different protocols and plasmids from the past few days. |
| + | Mentor Mike ultimately recommended them to run another gel on the PCR |
| + | product and the students diligently began the gel protocol. After a few hours, the |
| + | gel inspection seemed fine and the bands displayed exactly what the students expected. |
| + | Therefore Mentor Mike recommended that they re-do the ligation from Monday. The ligation |
| + | went very smoothly and the transformation will be done tomorrow.</p> |
| + | |
| + | <h3>Tuesday (8/8/17)</h3> |
| + | <p>Today the LabPats were finally able to transform the plasmid from the ligation |
| + | yesterday. They followed the standard protocol and transformed the cells into E. |
| + | coli DH5a again. After that, the cells were ready to be plated like previously. This |
| + | time the students hope that the cells will grow colonies and indicate the |
| + | completion of their "sense" component. The students left the lab in high spirits and |
| + | headed out for wifi and collaboration space to discuss more wiki work and future work.</p> |
| + | |
| + | <h3>Wednesday (8/9/17)</h3> |
| + | <p>The students eagerly went into the lab to check the plates from the |
| + | transformation. To the students dismay all the plates contained growth even the |
| + | blanks. The concerned students went to ask Mentor Mike, who help the student |
| + | decide to perform a PCR in order to run the product on a gel. They ran the gel |
| + | and got the desired band lengths for everything. They also noticed that some of |
| + | the colonies were already glowing green. With already putting in a long day of work the |
| + | students made overnights for the next day.</p> |
| + | |
| + | <h3>Thursday (8/10/17)</h3> |
| + | <p>Bright and early the students got their overnights out of the incubator. They then |
| + | made glycerol stocks and performed a mini prep, so the bacteria could be sent |
| + | for sequencing. In the afternoon, the students got to view a set of poster |
| + | presentations. This was especially helpful for the students, and gave them many |
| + | ideas on how to set up their poster.</p> |
| + | |
| + | <h3>Friday (8/11/17)</h3> |
| + | <p>Today was a sad day in the lab, as the summer was ending and the students |
| + | had to return to school. The team spent the morning taking their mentors to |
| + | breakfast, as a thank you for all they had done for us. The rest of the day was |
| + | spent at the lab. The students cleaned up their work benches, and made sure |
| + | everything was in order before they left the lab for the last time. The students said |
| + | |
| + | their good byes to their coworkers, whom they had enjoyed many lunch breaks with.</p> |
| + | </div> |
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− | <h3></h3>
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− | <p></p>
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