Team:NCTU Formosa/Collaborations
The new iGEM team- TCFSH
Since April 14th, NCTU_Formosa started to introduce experiments which is related to iGEM to the science-gifted class of Taichung First Senior High School(TCFSH). Gradually, the background knowledge of synthetic biology was adequate, then the cogitation of combination to engineering and biology was shared, the core of the gene regulation, the design of biobricks were taught. Also we introduce previous projects from NCTU_Formosa, hope to give them the prospect of the competition.
In the last semester, the instructor of NCTU_Formosa, Pro. Wen-Liang Chen, invited the gifted students from TCFSH who decided to learn about biology to our lab. NCTU_Formosa then gave them a series of training, the SOP of
our wet lab protocol, which includes:
These are basic experimental skills for people who studies biology and so as every iGEMers. Especially for the digestion part, while it is one of the most important part for iGEMers, we also taught them about the concept of biobrick assembly, the design of EXSP, different kinds of parts of biobrick… and so on.
And in this summer, we were glad to know that some TCFSH students decided to participate iGEM, new iGEMers were born. There are more things that a new team need to know, more knowledge to learn. NCTU_Formosa members taught
them more about :
We hope they could get more information in a short time and see the power of bioformatics, it’s also a concept of our project. Some of our members spent days with these high school students discussing about their ideas, and helped them to design experiments to realize their ideas. Later, they finally came out with their own biobrick sequences.
Through practicing and discussion, they completed many experiments on their own, including purification of plasmids, digestion with restriction enzyme, and conducting gel electrophoresis. They even came up with more outstanding thoughts about the project they’ve schematized this year. We are looking forward to the great shine of the team in Boston this year and get valuable experiences
The bidirectional cooperation with TCFSH_Taiwan
The team members of TCFSH_Taiwan helped us to do the double check on the correctness of our composite parts. They took our sample to run the Taq PCR and also ensured the band of each composite parts were correct via gel electrophoresis.
The team member of TCFSH_Taiwan, BO CHING, WU , was loading the Taq PCR products of our five composite part biobricks.
The result of gel electrophoresis is shown in the figure.
After comparison to the marker, they got the band size and did the double confirmation for our composite parts.
