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+ | <!-- example of slideshow of 1, ie. only a picture. If you wish to make slideshow again, remember to comment | ||
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+ | <img src="https://static.igem.org/mediawiki/2017/a/af/T--NTNU_Trondheim--Collaboration.jpg"> | ||
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+ | </div> | ||
− | < | + | <div class="content"> |
− | + | <div class="break_header"> | |
− | </ | + | <img src="https://static.igem.org/mediawiki/2017/4/49/T--NTNU_Trondheim--splitter_header.svg"> |
+ | </div> | ||
− | < | + | <div class="paragraph_no_img"> |
− | <p> | + | <h1>Collaboration</h1> |
− | + | <p> | |
− | </p> | + | We had a meet-up in Trondheim with the iGEM team from the University of Oslo (UiOslo). During the |
+ | meet-up we had a "Mini Jamboree" and held presentations for each other. Professor and previous iGEM | ||
+ | judge Eivind Almaas was present to give feedback, and the teams gave feedback and comments to the | ||
+ | presentation and project for each other. After the Mini Jamboree we had a lovely lunch together | ||
+ | (see pictures) and sightseeing in our laboratory, at the university campus, and in Trondheim city. | ||
+ | </p> | ||
+ | </div> | ||
− | <div class=" | + | <div class="image" style="width: 50%;margin-top: 5em"> |
+ | <!-- The width of the page the picture will occupy. The height is calculated from it to honor the source dimensions. Should NOT exceed 70%--> | ||
+ | <!-- margin-top and margin-bottom should be set to reach a suitable amount of whitespace, depending on the image location. 0 by default --> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/a/a5/T--NTNU_Trondheim--Mini_jamboree_with_UiO.jpg"> | ||
+ | <p>Presentation rehearsal at "mini-jamboree" with the UiOslo iGEM team.</p> | ||
+ | </div> | ||
− | < | + | <div class="image" style="width: 50%;margin-top: 5em"> |
− | <p> | + | <!-- The width of the page the picture will occupy. The height is calculated from it to honor the source dimensions. Should NOT exceed 70%--> |
− | + | <!-- margin-top and margin-bottom should be set to reach a suitable amount of whitespace, depending on the image location. 0 by default --> | |
− | </p> | + | <img src="https://static.igem.org/mediawiki/2017/6/6b/T--NTNU_Trondheim--Collaboration_lunch.jpg"> |
+ | <p>Lunch with the UiOslo iGEM team.<br><br><br><br></p> | ||
+ | </div> | ||
− | |||
− | |||
− | |||
− | </div> | + | <div class="break_2"> |
+ | <img src="https://static.igem.org/mediawiki/2017/5/52/T--NTNU_Trondheim--splitter_2.svg"> | ||
+ | </div> | ||
+ | <div class="paragraph_no_img"> | ||
+ | <h1>Improved characterization</h1> | ||
+ | <p> | ||
+ | Afterwards, we decided together that the UiOslo team could help our team by improving the | ||
+ | characterization of our biobrick MP6 mutator. They used <i>Escherichia coli</i> TOP10, which is a different | ||
+ | strain from the <i>E. coli</i> DH5α that we have been using. By using a different strain, we were able to obtain | ||
+ | more data on the mutagenesis activity of the plasmid and check if the plasmid worked in another strain. | ||
+ | <br><br> | ||
+ | We sent our biobrick to Oslo, together with the guidelines on how to perform the experiment. First, | ||
+ | the MP6 mutator plasmid was transformed into <i>E. coli</i> TOP10 together with the pLA230 control plasmid. | ||
+ | After 16 hours incubation at 37 C, the transformed bacteria culture was split into two, and glucose | ||
+ | (20 mM) was added in one culture flask while arabinose (20 mM) was added in the other one. The cultures | ||
+ | were incubated at 37 C for 24 hours. The next day, 100 μL of the different cultures were plated on plates | ||
+ | with LA, glucose and ampicillin (culture dilutions 10-0-10-3) and plates with only LA and glucose | ||
+ | (culture concentrations 10-5-10-8). The plates were grown overnight. The resulting plates are shown | ||
+ | in the picture below, and all results are also shown at Team UiOslo Norway's wiki at the | ||
+ | <a href="https://2017.igem.org/Team:UiOslo_Norway/Results">results page</a>. | ||
+ | </p> | ||
+ | </div> | ||
+ | <div class="image" style="width: 50%;margin-top: 5em"> | ||
+ | <!-- The width of the page the picture will occupy. The height is calculated from it to honor the source dimensions. Should NOT exceed 70%--> | ||
+ | <!-- margin-top and margin-bottom should be set to reach a suitable amount of whitespace, depending on the image location. 0 by default --> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/b/b4/T--NTNU_Trondheim--Collaboration_plates.jpg"> | ||
+ | <p>Plates from the UiOslo iGEM teams characterization of our biobrick.</p> | ||
+ | </div> | ||
− | <div class=" | + | <div class="paragraph_no_img"> |
− | <p> | + | <p> |
− | + | There were significant differences between the number of colonies formed in plates with cells and medium | |
− | </p> | + | that were preincubated with 20 mM arabinose and plates with cells and medium that were preincubated with |
+ | 20 mM glucose. | ||
+ | All plates containing Arabinose (MP6 inducer) had a higher number of colonies than the plates containing | ||
+ | Glucose (MP6 repressor). | ||
+ | <br><br> | ||
+ | MP6 induces mutations in the control plasmid pLA230, which originally contains a non-functional | ||
+ | Ampicillin resistance gene due to one point mutation. If the point mutation is reversed, this leads | ||
+ | to the cells gaining Ampicillin resistance. When grown on agra plates with Ampicillin, only cells that | ||
+ | got this point mutation reversed will be able to grow. The number of colonies on the plates, is therefore | ||
+ | an indirect measure of the mutation activity of MP6. | ||
+ | <br><br> | ||
+ | The results from the UiOslo iGEM team therefore confirmed that the biobrick works as expectated: | ||
+ | it has a high mutational activity if induced by arabinose, and a lower mutatinal activity if repressed | ||
+ | by glucose. Their results also confirmed that our biobrick can be used in E. coli Top10, which is a | ||
+ | different strain than we used. The numbers of colonies in the UiOslo teams experiments were of a higher | ||
+ | range than in our experiment, making it more difficult to count. This might be due to small procedural | ||
+ | differences, or it may indicate that the protocol for the test needs to be adjusted when using a different | ||
+ | strain than E. coli DH5α. | ||
+ | </p> | ||
+ | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
+ | <div class="break_1"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/6d/T--NTNU_Trondheim--splitter_1.svg"> | ||
+ | </div> | ||
+ | <div class="paragraph_no_img"> | ||
+ | <h1>Thank you!</h1> | ||
+ | <p> | ||
+ | We want to thank team UiOslo for the collaboration and a wonderful time at the "Mini Jamboree". | ||
+ | We are looking forward to meet again at the Giant Jamboree in Boston! | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="image" style="width: 50%;margin-top: 5em"> | ||
+ | <!-- The width of the page the picture will occupy. The height is calculated from it to honor the source dimensions. Should NOT exceed 70%--> | ||
+ | <!-- margin-top and margin-bottom should be set to reach a suitable amount of whitespace, depending on the image location. 0 by default --> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/a/a3/T--NTNU_Trondheim--Collaboration_office.jpg"> | ||
+ | <p>Team NTNU and team UiOslo at "mini-jamboree".<br><br><br><br></p> | ||
+ | </div> | ||
+ | |||
+ | <div class="footer"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/7/7c/T--NTNU_Trondheim--splitter_footer.svg"> | ||
+ | <div class="footer_content"> | ||
+ | <div class="sponsors"> | ||
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+ | </a> | ||
+ | <a href="http://www.ntnu.edu/ibt" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/8/83/T--NTNU_Trondheim--Instituttlogo_english_white.png"> | ||
+ | </a> | ||
+ | <a href="http://aquagen.no/en/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/2/27/T--NTNU_Trondheim--AquaGen_forslag1_transparent.png"> | ||
+ | </a> | ||
+ | <a href="http://www.biokjemisk.no/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/f/f4/T--NTNU_Trondheim--NBS_forslag1_transparent.png"> | ||
+ | </a> | ||
+ | <a href="http://www.imr.no/en" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/e/e4/T--NTNU_Trondheim--Havforskningsinstituttet_white.png"> | ||
+ | </a> | ||
+ | <a href="http://www.vectronbiosolutions.com/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/8/86/T--NTNU_Trondheim--Vectron_forslag1_transparent.png"> | ||
+ | </a> | ||
+ | <a href="http://digitallifenorway.org/gb/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/d/da/T--NTNU_Trondheim--SDL_LOGO_ENG_confirmed_version2.png"> | ||
+ | </a> | ||
+ | <a href="https://www.skretting.com/en-us/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/4/4d/T--NTNU_Trondheim--Skretting_logo_black.png"> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="sosial_media"> | ||
+ | <a href="mailto:igem-team@ntnu.no" > | ||
+ | <img src="https://static.igem.org/mediawiki/2017/5/5a/T--NTNU_Trondheim--mail.png"> | ||
+ | </a> | ||
+ | <a href="http://www.facebook.com/igemntnu2017/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/8/8b/T--NTNU_Trondheim--facebook.png"> | ||
+ | </a> | ||
+ | <a href="http://twitter.com/ntnuigem?lang=en/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/8/8b/T--NTNU_Trondheim--twitter.png"> | ||
+ | </a> | ||
+ | <a href="http://www.instagram.com/ntnu_igem/" target="_blank"> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/64/T--NTNU_Trondheim--instagram.png"> | ||
+ | </a> | ||
+ | <p> | ||
+ | Email: igem-team@ntnu.no | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </body> | ||
</html> | </html> |
Revision as of 18:52, 1 November 2017
Collaboration
We had a meet-up in Trondheim with the iGEM team from the University of Oslo (UiOslo). During the meet-up we had a "Mini Jamboree" and held presentations for each other. Professor and previous iGEM judge Eivind Almaas was present to give feedback, and the teams gave feedback and comments to the presentation and project for each other. After the Mini Jamboree we had a lovely lunch together (see pictures) and sightseeing in our laboratory, at the university campus, and in Trondheim city.
Presentation rehearsal at "mini-jamboree" with the UiOslo iGEM team.
Lunch with the UiOslo iGEM team.
Improved characterization
Afterwards, we decided together that the UiOslo team could help our team by improving the
characterization of our biobrick MP6 mutator. They used Escherichia coli TOP10, which is a different
strain from the E. coli DH5α that we have been using. By using a different strain, we were able to obtain
more data on the mutagenesis activity of the plasmid and check if the plasmid worked in another strain.
We sent our biobrick to Oslo, together with the guidelines on how to perform the experiment. First,
the MP6 mutator plasmid was transformed into E. coli TOP10 together with the pLA230 control plasmid.
After 16 hours incubation at 37 C, the transformed bacteria culture was split into two, and glucose
(20 mM) was added in one culture flask while arabinose (20 mM) was added in the other one. The cultures
were incubated at 37 C for 24 hours. The next day, 100 μL of the different cultures were plated on plates
with LA, glucose and ampicillin (culture dilutions 10-0-10-3) and plates with only LA and glucose
(culture concentrations 10-5-10-8). The plates were grown overnight. The resulting plates are shown
in the picture below, and all results are also shown at Team UiOslo Norway's wiki at the
results page.
Plates from the UiOslo iGEM teams characterization of our biobrick.
There were significant differences between the number of colonies formed in plates with cells and medium
that were preincubated with 20 mM arabinose and plates with cells and medium that were preincubated with
20 mM glucose.
All plates containing Arabinose (MP6 inducer) had a higher number of colonies than the plates containing
Glucose (MP6 repressor).
MP6 induces mutations in the control plasmid pLA230, which originally contains a non-functional
Ampicillin resistance gene due to one point mutation. If the point mutation is reversed, this leads
to the cells gaining Ampicillin resistance. When grown on agra plates with Ampicillin, only cells that
got this point mutation reversed will be able to grow. The number of colonies on the plates, is therefore
an indirect measure of the mutation activity of MP6.
The results from the UiOslo iGEM team therefore confirmed that the biobrick works as expectated:
it has a high mutational activity if induced by arabinose, and a lower mutatinal activity if repressed
by glucose. Their results also confirmed that our biobrick can be used in E. coli Top10, which is a
different strain than we used. The numbers of colonies in the UiOslo teams experiments were of a higher
range than in our experiment, making it more difficult to count. This might be due to small procedural
differences, or it may indicate that the protocol for the test needs to be adjusted when using a different
strain than E. coli DH5α.
Thank you!
We want to thank team UiOslo for the collaboration and a wonderful time at the "Mini Jamboree". We are looking forward to meet again at the Giant Jamboree in Boston!
Team NTNU and team UiOslo at "mini-jamboree".