Difference between revisions of "Team:NCTU Formosa/Collaborations"

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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Collaborations</h1>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
 
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<h3>Silver Medal Criterion #2</h3>
 
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Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
 
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<h4> Which other teams can we work with? </h4>
 
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
 
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In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
 
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Here are some suggestions for projects you could work on with other teams:
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<ul>
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<body>
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulating another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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</ul>
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            <h1>The new iGEM team- TCFSH</h1>
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            <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Since April 14th, NCTU_Formosa started to introduce experiments which is related to iGEM to the science-gifted class of Taichung First Senior High School(TCFSH). Gradually, the background knowledge of synthetic biology was
 +
                adequate, then the cogitation of combination to engineering and biology was shared, the core of the gene regulation, the design of biobricks were taught. Also we introduce previous projects from NCTU_Formosa, hope to give them the prospect
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                of the competition.
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            </p>
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            <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In the last semester, the instructor of NCTU_Formosa, Pro. Wen-Liang Chen, invited the gifted students from TCFSH who decided to learn about biology to our lab. NCTU_Formosa then gave them a series of training, the SOP of
 +
                our wet lab protocol, which includes:
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                <span><ul>
 +
                <li>Cultivation of E.Coli</li>
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                <li>Miniprep</li>
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                <li>Digestion</li>
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                <li>Ligation</li>
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                <li>Transformation</li>
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                <li>PCR</li>
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                <li>Gel Electrophoresis</li>
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            </ul></span>
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            </p>
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            <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;These are basic experimental skills for people who studies biology and so as every iGEMers. Especially for the digestion part, while it is one of the most important part for iGEMers, we also taught them about the concept
 +
                of biobrick assembly, the design of EXSP, different kinds of parts of biobrick… and so on.
 +
            </p>
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            <p>
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;And in this summer, we were glad to know that some TCFSH students decided to participate iGEM, new iGEMers were born. There are more things that a new team need to know, more knowledge to learn. NCTU_Formosa members taught
 +
                them more about :
 +
                <span><ul>
 +
                <li>Useful databases, such as NCBI, Uniport</li>
 +
                <li>Useful databases, such as NCBI, Uniport</li>
 +
                <li>Useful tools for protein, such as BLAST, COBALT, CD search</li>
 +
                <li>Biology experiment design and debug</li>
 +
                <li>Writing Biobricks for synthesis (in sequences)</li>
 +
            </ul></span>
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            </p>
 +
 +
            <p>
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;We hope they could get more information in a short time and see the power of bioformatics, it’s also a concept of our project. Some of our members spent days with these high school students discussing about their ideas, and
 +
                helped them to design experiments to realize their ideas. Later, they finally came out with their own biobrick sequences.
 +
            </p>
 +
 +
            <p>
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Through practicing and discussion, they completed many experiments on their own, including purification of plasmids, digestion with restriction enzyme, and conducting gel electrophoresis. They even came up with more outstanding
 +
                thoughts about the project they’ve schematized this year. We are looking forward to the great shine of the team in Boston this year and get valuable experiences
 +
            </p>
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        </div>
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        <div>
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            <hi>The bidirectional cooperation with TCFSH_Taiwan </hi>
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            <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The team members of TCFSH_Taiwan helped us to do the double check on the correctness of our composite parts. They took our sample to run the Taq PCR and also ensured the band of each composite parts were correct via gel electrophoresis.
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            </p>
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            <img class="collab_photo" src="#" width="60vw">
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            <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The team member of TCFSH_Taiwan, BO CHING, WU , was loading the Taq PCR products of our five composite part biobricks.
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            </p>
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            <img class="collab_photo" src="#" width="60vw">
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            <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The result of gel electrophoresis is shown in the figure.
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            </p>
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            <img class="collab_photo" src="#" width="60vw">
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            <p>
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;After comparison to the marker, they got the band size and did the double confirmation for our composite parts.
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            </p>
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Revision as of 15:46, 26 October 2017

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JUDGING FORM
NCTU_Formosa: Collaborations

The new iGEM team- TCFSH

     Since April 14th, NCTU_Formosa started to introduce experiments which is related to iGEM to the science-gifted class of Taichung First Senior High School(TCFSH). Gradually, the background knowledge of synthetic biology was adequate, then the cogitation of combination to engineering and biology was shared, the core of the gene regulation, the design of biobricks were taught. Also we introduce previous projects from NCTU_Formosa, hope to give them the prospect of the competition.

     In the last semester, the instructor of NCTU_Formosa, Pro. Wen-Liang Chen, invited the gifted students from TCFSH who decided to learn about biology to our lab. NCTU_Formosa then gave them a series of training, the SOP of our wet lab protocol, which includes:

  • Cultivation of E.Coli
  • Miniprep
  • Digestion
  • Ligation
  • Transformation
  • PCR
  • Gel Electrophoresis

     These are basic experimental skills for people who studies biology and so as every iGEMers. Especially for the digestion part, while it is one of the most important part for iGEMers, we also taught them about the concept of biobrick assembly, the design of EXSP, different kinds of parts of biobrick… and so on.

     And in this summer, we were glad to know that some TCFSH students decided to participate iGEM, new iGEMers were born. There are more things that a new team need to know, more knowledge to learn. NCTU_Formosa members taught them more about :

  • Useful databases, such as NCBI, Uniport
  • Useful databases, such as NCBI, Uniport
  • Useful tools for protein, such as BLAST, COBALT, CD search
  • Biology experiment design and debug
  • Writing Biobricks for synthesis (in sequences)

     We hope they could get more information in a short time and see the power of bioformatics, it’s also a concept of our project. Some of our members spent days with these high school students discussing about their ideas, and helped them to design experiments to realize their ideas. Later, they finally came out with their own biobrick sequences.

     Through practicing and discussion, they completed many experiments on their own, including purification of plasmids, digestion with restriction enzyme, and conducting gel electrophoresis. They even came up with more outstanding thoughts about the project they’ve schematized this year. We are looking forward to the great shine of the team in Boston this year and get valuable experiences

The bidirectional cooperation with TCFSH_Taiwan

     The team members of TCFSH_Taiwan helped us to do the double check on the correctness of our composite parts. They took our sample to run the Taq PCR and also ensured the band of each composite parts were correct via gel electrophoresis.

     The team member of TCFSH_Taiwan, BO CHING, WU , was loading the Taq PCR products of our five composite part biobricks.

     The result of gel electrophoresis is shown in the figure.

     After comparison to the marker, they got the band size and did the double confirmation for our composite parts.

Untitled Document